Molecular detection of Borrelia theileri in cattle in Korea
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초록
Bovine borreliosis, caused by Borrelia theileri which is transmitted via hard tick bites, isassociated with mild clinical symptoms, such as fever, lethargy, hemoglobinuria, anorexia, and anemia. Borrelia theileri infects various animals, such as cattle, deer, horses,goats, sheep, and wild ruminants, in Africa, Australia, and South America. Notably, nocase of B. theileri infection has been reported in Korean cattle to date. In this study, 101blood samples were collected from a Korean indigenous cattle breed, among which1.98% tested positive for B. theileri via nested PCR. The obtained sequences exhibitedhigh homology with B. theileri strains identified in other regions. Phylogenetic analysis of16S rRNA confirmed the B. theileri group affiliation; however, flagellin B sequences exhibited divergence, potentially due to regional evolutionary differences. This study provides the first molecular confirmation of B. theileri infection in Korean livestock. Furtherisolation and nucleotide sequence analyses are necessary to better understand thepresence of B. theileri strains in cows in Korea.
Abstract
Bovine borreliosis, caused by Borrelia theileri which is transmitted via hard tick bites, isassociated with mild clinical symptoms, such as fever, lethargy, hemoglobinuria, anorexia, and anemia. Borrelia theileri infects various animals, such as cattle, deer, horses,goats, sheep, and wild ruminants, in Africa, Australia, and South America. Notably, nocase of B. theileri infection has been reported in Korean cattle to date. In this study, 101blood samples were collected from a Korean indigenous cattle breed, among which1.98% tested positive for B. theileri via nested PCR. The obtained sequences exhibitedhigh homology with B. theileri strains identified in other regions. Phylogenetic analysis of16S rRNA confirmed the B. theileri group affiliation; however, flagellin B sequences exhibited divergence, potentially due to regional evolutionary differences. This study provides the first molecular confirmation of B. theileri infection in Korean livestock. Furtherisolation and nucleotide sequence analyses are necessary to better understand thepresence of B. theileri strains in cows in Korea.
참고문헌 (17)
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