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16S rDNA클론 Libraries를 이용한 치근단 농양 병소의 세균 동정

Identification of Bacteria from Periapical Abscess Using 16S rDNA Clone Libraries.

오상호|임성훈|국중기|김화숙|황호길|민정범|유소영|김미광|김평식
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2004.06|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제32권 제2호|pp. 195-198

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참고문헌

주제분류생물학

초록

Abstract

Molecular analysis was performed on the microflora found in the necrotic pulpal tissue collected from 5 infected root canals that were diagnosed as a periapical abscess. 16S rRNA coding gene (rDNA) library construction and sequencing were performed in order to identify the microflora, The 16S rDNA sequences from 278 clones were identified by a comparison with the database sequence in GenBank. Three phylum and 31 species, which were related to the oral microflora, were identified from the 3 samples (No. 87, 105, and 115). Dialister invisus (5.6%), Peptostreptococcus micron (18.3%), and Veillonella sp. (3.3%) were the organism present in all three samples. Lactobacillus fermentum (2.8%), Eubacterum sp./E. infirmum (6.7%), Shuttleworthia satelles (3.9%), Psudoramibacter alactolyticus (13.3%), Bulleidia moorei (2.8%), and Prevotella denticola (1.1%) were found in two samples. Two phylum and four species of environmental microflora were identified from 2 samples (No. 95 and 101). The reason for this might be contamination of the samples with dental water. These results showed that molecular analysis could reveal more diverse microflora that are associated with endodontic infections than that revealed by conventional cultural methods. In addition, these results may offer the basic data to epidemiological studies related with endodontic infection.

참고문헌 (53)

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관련 논문AI추천

중합효소연쇄반응법을 이용한 급성 치수 및 치근단 질환의 병원성 세균의 동정국중기, 김지훈, 박슬희, 유소영, 임상수, 임선아, 황호길대한치과보존학회 · Restorative Dentistry and Endodontics2003 · 제28권 제2호감염 근관에서 중합효소연쇄반응법을 이용한 Eubacterium 균종의 동정금기연, A.F.Fouad대한치과보존학회 · Restorative Dentistry and Endodontics2003 · 제28권 제3호급성 치수염 및 급성 치근단 농양의 치근관으로부터의 세균 분리 및 동정국중기, 김미광, 이연재, 황호길대한치과보존학회 · Restorative Dentistry and Endodontics2005 · 제30권 제5호16S rDNA 클론 library 제작 및 핵산염기서열 결정을 통한 교정용 미니임플랜트 주위 열구의 세균 동정국중기, 김광원, 유소영, 임성훈, 장영일대한치과교정학회 · 대한치과교정학회지2006 · 제36권 제4호근관감염세균의 분포에 관한 연구김승윤, 박상혁, 최기운, 최호영대한치과보존학회 · Restorative Dentistry and Endodontics2003 · 제28권 제4호원발성 치근단 치주염을 갖는 감염근관에서 증상유무에 따른 세균분포의 pyrosequencing 분석이윤, 금기연, 김은정, 박준홍, 배광식, 이태권, 임상민대한치과보존학회 · Restorative Dentistry and Endodontics2011 · 제36권 제6호

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