Isolation of Novel Taxa Using Complex Media with Influent Sewage Water
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, we evaluated complex media with influent sewage water (ISW) to isolate novel taxa of bacteria. It was possible to cultivate 13 genera using the complex media with ISW. Additionally, more diverse genera were identified at 37°C than at 25°C, using the complex media with ISW. Total 12 strains of 179 bacterial isolates were shared less than 97% 16S rRNA gene sequence similarities with any known species. These isolates could be assigned to genera Tessaracoccus, Paracoccus, or Candidimonas (or Paralcaligenes).
Abstract
In this study, we evaluated complex media with influent sewage water (ISW) to isolate novel taxa of bacteria. It was possible to cultivate 13 genera using the complex media with ISW. Additionally, more diverse genera were identified at 37°C than at 25°C, using the complex media with ISW. Total 12 strains of 179 bacterial isolates were shared less than 97% 16S rRNA gene sequence similarities with any known species. These isolates could be assigned to genera Tessaracoccus, Paracoccus, or Candidimonas (or Paralcaligenes).
참고문헌 (15)
- Chun J, "Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences", Antonie van Leeuwenhoek, 78, pp.123~127 (2000)
- Chung WY, "A study on the coliforms removal methods at wastewater treatment plants", Master, pp.2003
- Hall TA, "Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95", 98, Nucleic Acids Symp. Ser, pp.1999, 41[DOI]
- 홍정희, "광합성세균 배양액이 음식물쓰레기에 미치는 영향", 한국폐기물자원순환학회지, 22(2), pp.113~119 (2005), 한국폐기물자원순환학회
- Jiang X, "Bacterial diversity of active sludge in wastewater treatment plant", Earth Sci. Fron, 15, pp.163~168 (2008)
- 김도형, "난배양성 토양세균의 배양법 평가 및 신 분류군의 순수분리", 미생물학회지, 44(4), pp.352~357 (2008), 한국미생물학회
- 김미선, "미생물에 의한 수소생산: Dark Anaerobic Fermentation and Photo-biological Process", KSBB Journal, 20(6), pp.393~400 (2005), 한국생물공학회
- Kim OS, "Introducing EzTaxon: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species", Int. J. Syst. Evol. Microbiol, 62, pp.716~721 (2012)
- Lee S, "Identification and characterization of Methylobacterium dankookense sp. nov. isolated from drinking water", Mas-ter, pp.2010
- 이시원, "환경 유형에 따른 바이오에어로졸 중 배양성 세균 동정 및 계통분석", 한국미생물·생명공학회지, 43(2), pp.142~149 (2015), 한국미생물·생명공학회[DOI]
- Lee SA, "Taxonomic study of denitrifying bacteria isolated from Daejeon sewage treatment plan", Master, pp.2003
- Sánchez O, "Assessing bacterial diversity in a seawater-processing wastewater treatment plant by 454-pyrosequencing of the 16S rRNA and amoA genes", Microb. Biotechnol, 6, pp.435~442 (2013)
- Tamaki H, "Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques", Appl. Environ. Microbiol, 71, pp.2162~2169 (2005)
- Tamura K, "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol. Biol. Evol, 28, pp.2731~2739 (2011)
- Wang X, "Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China", Appl. Environ. Microbiol, 78, pp.7042~7047 (2012)