Phenazine-1-carboxamide, an Extrolite Produced by Pseudomonas aeruginosa Strain (CGK-KS-1) Isolated from Ladakh and India, and its Evaluation Against Various Xanthomonas spp.
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In the enduring investigation of the bioactive microbes, Pseudomonas aeruginosa strain (referred to as CGK-KS-1 (ICTB-315)), isolated from Chumathang hot spring, Ladakh, and India, was identified to possess a major bioactive fraction with antimicrobial and anti-biofilm properties. This bioactive metabolite was purified through bioactivity-guided fractionation. The chemical structure of this major compound was elucidated as phenazine-1-carboxamide (PCN) based on 1H and 13C NMR, FT-IR, EI-HR-MS and 2D NMR spectroscopic techniques. In the current study, PCN exhibited antimicrobial activity with MIC values ranging between 1.9−3.9 μg/ml against various test human pathogens and Xanthomonas spp. PCN showed the antibiofilm property with the IC50 values ranging from 17.04 to 60.7 μM against different test pathogens. The in silico docking studies showed PCN strongly interacted with various proteins of different Xanthomonas spp. with high binding energies. We report herein for the first time the anti-biofilm property and the docking studies of PCN. The extrolite from P. aeruginosa strain CGK-KS-1 showed promising bioactivities and may be considered as a potential candidate for application in various biocontrol strategies.
Abstract
In the enduring investigation of the bioactive microbes, Pseudomonas aeruginosa strain (referred to as CGK-KS-1 (ICTB-315)), isolated from Chumathang hot spring, Ladakh, and India, was identified to possess a major bioactive fraction with antimicrobial and anti-biofilm properties. This bioactive metabolite was purified through bioactivity-guided fractionation. The chemical structure of this major compound was elucidated as phenazine-1-carboxamide (PCN) based on 1H and 13C NMR, FT-IR, EI-HR-MS and 2D NMR spectroscopic techniques. In the current study, PCN exhibited antimicrobial activity with MIC values ranging between 1.9−3.9 μg/ml against various test human pathogens and Xanthomonas spp. PCN showed the antibiofilm property with the IC50 values ranging from 17.04 to 60.7 μM against different test pathogens. The in silico docking studies showed PCN strongly interacted with various proteins of different Xanthomonas spp. with high binding energies. We report herein for the first time the anti-biofilm property and the docking studies of PCN. The extrolite from P. aeruginosa strain CGK-KS-1 showed promising bioactivities and may be considered as a potential candidate for application in various biocontrol strategies.
참고문헌 (32)
- Kumar CG, "Biodirected synthesis of Miconazole-conjugated bacterial silver nanoparticles and their application as antifungal agents and drug delivery vehicles", Colloids Surf. B Biointerfaces, 125, pp.110~119 (2015)
- Maida I, "Phenotypic and genomic characterization of the Antarctic bacterium Gillisia sp. CAL575, a producer of antimicrobial compounds", Extremophiles, 18, pp.35~49 (2014)
- Tedesco P, "Antimicrobial activity of monorhamnolipids produced by bacterial strains isolated from the Ross Sea (Antarctica)", Mar. Drugs, 14, pp.83~ (2016)
- K. K. Pal, "Biological Control of Plant Pathogens", The Plant Health Instructor (2006), Scientific Societies[DOI]
- Tomihama T, "Biofilm formation and resistance to bactericides of Pseudomonas syringae pv. theae", J. Gen. Plant Pathol, 73, pp.193~196 (2007)
- Purcell AH, "Fastidious xylem-limited bacterial plant pathogens", Annu. Rev. Phytopathol, 34, pp.131~151 (1996)
- Crossman L, "Biofilm formation and dispersal in Xanthomonas campestris", Microbes Infect, 6, pp.623~629 (2004)
- Kang Y, "Ralstonia solanacearum requires type 4 pili to adhere to multiple surfaces and for natural transformation and virulence", Mol. Microbiol, 46, pp.427~437 (2002)
- Costerton JW, "Biofilm in implant infections: its production and regulation", Int. J. Altif. Organs, 28, pp.1062~1068 (2005)
- Rizvi J, "Ladakh: Crossroads of High Asia" (1996), Oxford University Press
- Joshi PK, "Biodiversity characterization in Nubra Valley, Ladakh with special reference to plant resource conservation and bioprospecting", Biodivers. Conserv, 15, pp.4253~4270 (2006)
- Ghosh A, "Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil", Int. J. Syst. Evol. Microbiol, 57, pp.238~242 (2007)
- Kumar, C. Ganesh, "Decolorization and Biotransformation of Triphenylmethane Dye, Methyl Violet, by Aspergillus sp. Isolated from Ladakh, India", Journal of Microbiology and Biotechnology, 21(3), pp.267~273 (2011), 한국미생물·생명공학회
- Ahmed Kamal, "Metabolic Profiling and Biological Activities of Bioactive Compounds Produced by Pseudomonas sp. Strain ICTB-745 Isolated from Ladakh, India", Journal of Microbiology and Biotechnology, 22(1), pp.69~79 (2012), 한국미생물·생명공학회
- Shanmugaiah V, "Purification, crystal structure and antimicrobial activity of phenazine-1-carboxamide produced by a growth-promoting biocontrol bacterium, Pseudomonas aeruginosa MML2212", J. Appl. Microbiol, 108, pp.703~711 (2010)
- Cardozo VF, "Antibacterial activity of extracellular compounds produced by a Pseudomonas strain against methicillin-resistant Staphylococcus aureus (MRSA) strains", Ann. Clin. Microbiol. Antimicrob, 12, pp.12~ (2013)
- Tupe SG, "Possible mechanism of antifungal phenazine-1-carboxamide from Pseudomonas sp. against dimorphic fungi Benjaminiella poitrasii and human pathogen Candida albicans", J. Appl. Microbiol, 118, pp.39~48 (2015)
- Amsterdam D, "Antibiotics in Laboratory Medicine", pp.52~111 (1966), Williams and Wilkins
- Wayne PA, "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 10th Ed", CLSI document M-07-A10, ~ (2015)
- Christensen GD, "Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices", J. Clin. Microbiol, 22, pp.996~1006 (1985)
- Morris GM, "Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function", J. Comput. Chem, 19, pp.1639~1662 (1998)
- Römer A, "1H NMR spectra of substituted phenazines", Org. Magn. Reson, 19, pp.66~68 (1982)
- Chin-A-Woeng TFC, "Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici", Mol. Plant Microbe. Interact, 11, pp.1069~1077 (1998)
- Hall-Stoodley L, "Bacterial biofilms:from natural environments to infectious diseases", Nat. Rev. Microbiol, 2, pp.95~108 (2004)
- Høiby N, "Antibiotic resistance of bacterial biofilms", Int. J. Antimicrob. Agents, 35, pp.322~332 (2010)
- Zhu PL, "The rsmA-like gene rsmAXoo of Xanthomonas oryzae pv. oryzae regulates bacterial virulence and production of diffusible signal factor", Mol. Plant Pathol, 12, pp.227~237 (2011)
- Zhang Y, "The HDGYP domain protein RpfG of Xanthomonas oryzae pv. oryzicola regulates synthesis of extracellular polysaccharides that contribute to biofilm formation and virulence on rice", PLoS One, 8, pp.e59428~ (2013)
- Dunger G, "Xanthomonas citri subsp. citri type IV pilus is required for twitching motility, biofilm development, and adherence", Mol. Plant Microbe Interact, 27, pp.1132~1147 (2014)
- Rigano LA, "Biofilm formation, epiphytic fitness, and canker development in Xanthomonas axonopodis pv. citri", Mol. Plant Microbe Interact, 20, pp.1222~1230 (2007)
- Li J, "The wxacO gene of Xanthomonas citri ssp. citri encodes a protein with a role in lipopolysaccharide biosynthesis, biofilm formation, stress tolerance and virulence", Mol. Plant Pathol, 12, pp.381~396 (2011)
- Castiblanco LF, "New insights on molecular regulation of biofilm formation in plant-associated bacteria", J. Integr. Plant Biol, 58, pp.362~372 (2016)
- Laursen JB, "Phenazine natural products: biosynthesis, synthetic analogues, and biological activity", Chem. Rev, 104, pp.1663~1686 (2004)