한천분해 미생물 Vibrio sp. GNUM08123의 동정 및 agarase 생산의 발효적 특성
Identification and Characterization of Agar-degrading Vibrio sp. GNUM08123 Isolated from Marine Red Macroalgae
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초록
GNUM08123 균주는 아가레이즈 생산 균주로서 대한민국동해안 영일만 해안가에 서식하는 홍조류로부터 분리되었다. GNUM08123은 그램-음성, 호기성 박테리아로 베이지색소를내며, C16:0 (25.9%)와 summed feature 3 (C16:1ω7c/iso-C15:02- OH, 34.4%)가 주요한 세포지방산 성분이다. 16S rRNA 유전자 서열 분석에 의하면, GNUM08123는 Gammaproteobacteria에속하며, V. chagasii R-3712T와 97.7%의 가장 높은 유사성을 갖는다. GNUM08123의 DNA G+C 함량은46.9 mol%이며, 주요한 isoprenoid quinone은 ubiquinone- 8이다. DNA-DNA hybridization 결과 및 16S rRNA 유전자서열 유사성, 형태학적·생리화학적 특성 등에 기초하여, GNUM08123은 가장 근접한 표준균주인 V. chagasii R- 3712T와는 구분되는 Vibrio속의 신종으로 판단되며, Vibrio sp. GNUM08123으로 명명하였다. GNUM08123에 의한 아가레이즈 생산은 아가와 설탕에 의해서는 유도되지만 포도당과 맥아당에 의해서는 억제되는데, 이는 CCR에 의한 것으로 판단된다.
Abstract
An agar-degrading bacterium, designated as the GNUM08123 strain, was isolated from samples of red algae collected from the Yongil Bay near East Sea, Korea. The isolated GNUM08123 strain was gram-negative, aerobic, motile, and beige-pigmented, with C16:0 (25.9%) and summed feature 3 (comprising C16:1ω7c/iso- C15:02-OH, 34.4%) as its major cellular fatty acids. A similarity search based on the 16S rRNA gene sequence revealed that it belonged to class Gammaproteobacteria and shared 97.7% similarity with the type strain Vibrio chagasii R-3712T. The DNA G+C content of strain GNUM08123T was 46.9 mol%. The major isoprenoid quinone was ubiquinone-8. The results of DNA–DNA relatedness and 16S rRNA sequence similarity analyses, in addition to its phenotypic and chemotaxonomic characteristics, suggest that strain GNUM08123 is a novel species within genus Vibrio, designated as Vibrio sp. GNUM08123. Agarase production by strain GNUM08123 was induced by agar and sucrose, but was repressed probably owing to carbon catabolite repression by glucose and maltose.
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