Corynebacterium lactofermentum에서 Bacillus subtilis의 Mannanase 유전자 발현
Expression of a Bacillus subtilis Mannanase Gene in Corynebacterium lactofementum
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A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli - Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract.The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.
Abstract
A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli - Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract.The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.
참고문헌 (13)
- Adham, S. A, "Improved vectors for transcriptional", translational signal screening in corynebacteria using the melC operon from Streptomyces glaucescens as reporter, 2003, pp.180, 53~59
- Bischoff, K. M, "Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361", Proc. Biochem, 42, pp.1150~1154
- Deb, J. K, "Intergeneric protoplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidophilum ATCC 21476", FEMS Microbiol. Lett., 59, pp.287~292
- Lee, K. -N, "Construction and transformation of an endogenous plasmid pBL1-free Brevibacterium lactofermentum", Kor. J. Appl. Microbiol. Biotechnol, 23, pp.164~169 (1995)
- Mladen, V, "Phytate utilization by genetically engineered lysine-producing Corynebacterium glutamicum", J. Biotechnol., 134, pp.211~217 (2008)
- 오영필, "Mannanase를 생산하는 Bacillus sp. WL-3 균주의 분리와 효소 새산성", 한국미생물·생명공학회지, 30(3), pp.247~252 (2002), 한국미생물·생명공학회
- Seibold, G, "Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: growth and lysine production", J. Biotechnol, 127, pp.381~391 (2006)
- Smith, M. D, "Protoplast transformation in coryneform bacteria and introduction of an α-amylase gene from Bacillus amyloliquefaciens into Brevibacterium lactofermentum", Appl. Environ. Microbiol., 51, pp.634~639 (1986)
- Tateno, T, "Production of L-Lysine from starch by Corynebacterium glutamicum displaying α-amylase on its cell surface", Appl. Microbiol. Biotechnol, 74, pp.1213~1220 (2007)
- Tateno, T, "Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis α-amylase using cspB promoter and signal sequence", Appl. Microbiol. Biotechnol, 77, pp.533~541 (2007)
- Watanabe, K, "Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences", Microbiol, 155, pp.741~750 (2009)
- YOON, KI-HONG, "Cloning and Strong Expression of a Bacillus subtilis WL-3 Mannanase Gene in B. subtilis", Journal of Microbiology and Biotechnology, 17(10), pp.1688~1694 (2007), 한국미생물·생명공학회
- Ki-Hong Yoon, "Characterization of the Bacillus subtilis WL-3 Mannanase from a Recombinant Escherichia coli", The Journal of Microbiology, 46(3), pp.344~349 (2008), 한국미생물학회