고염에서 생장하는 젓갈 유래 Bacteria의 분리 및 고염에서의 생육 특성
Isolation of Bacteria from Jeotgal Using High-salt-content Media and Their Growths in High-salt Condition.
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젓갈의 숙성에 미치는 bacteria의 역할 규명을 목표로 고염에서 생장하며 단백질 분해활성을 나타내는 bacteria를 멸치젓과 새우젓으로부터 분리하여 이들의 고염에서의 생장을검토하였다. NaCl이 15% 첨가된 고체배지를 이용하여bacteria를 분리한 경우, 멸치젓으로부터는 Bacillus 및 근연속이, 새우젓으로부터는 Staphylococcus 속이 우점으로 분리되었고, 멸치젓으로부터 분리된 Virgibacillus halodenitrificans 와 새우젓으로부터 분리된 Halobacillus trueperi가 단백질분해활성을 나타내었다. NaCl이 8% 첨가된 고체배지에서단백질 분해활성을 나타낸 멸치젓 유래 bacteria는 Vb. halodenitrificans를 중심으로 한 Bacillus 근연 속으로 NaCl 농도 15%에서 생장하는 bacteria의 군집과 크게 다르지 않았다. 그러나 NaCl이 8% 첨가된 고체배지에서 단백질 분해활성을 나타낸 새우젓 유래 bacteria는 NaCl 농도 15%에서분리된 우점균 Staphylococcus, Salinicoccus, Salimicrobium 속이 아닌 Bacillus 속과 Planococcus, Salinivibrio 속으로확인되어 새우젓의 우점종은 단백질 분해와 큰 관련이 없는것으로 추정된다. 멸치젓의 우점종 Vb. halodenitrificans와새우젓의 우점종 Staphylococcus equorum은 NaCl이 25%첨가된 배지에서도 생장을 나타내어 젓갈 숙성과 높은 관련성을 가지고 있으며, 종균으로 이용될 높은 가능성을 가지고 있다.
Abstract
Proteolytic bacteria were isolated from Myeolchi-jeotgal and Saeu-jeotgal using high-salt-content media and their growths in the media containing 25% NaCl were monitored to draw the role of bacteria in the ripening of jeotgal. The most populous genus in Myeolchi-jeotgal detected on agar media with 15% NaCl was Bacillus and its relatives, while the most populous in Saeu-jeotgal was Staphylococcus. Among the isolates, Virgibacillus halodenitrificans from Myeolchi-jeotgal and Halobacillus trueperi from Saeu-jeotgal showed proteinase activities. The species from Myeolchi-jeotgal showed proteinase activity on the agar media with 8% NaCl were similar to those isolated from the media with 15% NaCl. The dominant of Myeolchi-jeotgal isolated at the 15% NaCl concentration may be involved in the proteolysis. The proteolytic species from Saeu-jeotgal on the agar media with 8% NaCl were the genera Bacillus, Salinicoccus, and Salimicrobium those were not the dominants at 15% NaCl condition. The dominant isolates from Saeu-jeotgal on agar media with 15% NaCl may not be involved in the proteolysis of Saeu-jeotgal. Vb. halodenitrificans and Staphylococcus equorum, the dominant species from Myeolchi-jeotgal and Saeu-jeotgal, showed growths at the nutrient broth containing 25% NaCl. They may play a significant role in the ripening of jeotgal and have a high possibility to be used as the starter.
참고문헌 (31)
- Ahn, Y.-S., "Production ofprotease by the extreme halophile, Halobacterium sp", J.Korean Agric. Chem. Soc, 33, pp.247~251 (1990)
- Cha, Y.-J., "Studies on the processing of low salt fermented sea foods (2.The taste compounds of low salt fermented sardine)", Bull.Korean Fish. Soc, 16, pp.140~146 (1983)
- Chun, J., "EzTaxon: a web-based tool for theidentification of prokaryotes based on 16S ribosomal RNAgene sequences", Int. J. Syst. Evol. Microbiol, 57, pp.2259~2261 (2007)
- Evers, D. J, "Ensiling salt-preservedshrimp waste with grass straw and molasses", Anim. FeedSci. Technol, 71, pp.241~249 (1998)
- Franca, L., "Salinicoccus salsiraiae sp. nov.: a new moderatelyhalophilic gram-positive bacterium isolated from saltedskate", Extremophiles, 10, pp.531~536 (2006)
- Fukami, K., "Characterization and distribution of Staphylococcussp. implicated for improvement of fish sauce odor", Fisheries Sci, 70, pp.916~923 (2004)
- Fukami, K., "Improvement of fish-sauce odor by treatment with bacteriaisolated from the fish-sauce mush (Moromi) made fromfrigate mackerel", J. Food Sci, 69, pp.45~49 (2004)
- Guan, L., "Analysis of thecultivable bacterial community in jeotgal, a Korean saltedand fermented seafood, and identification of its dominantbacteria", Food Microbiol, 28, pp.101~113 (2011)
- Hur,S.H, "Critical review on the microbiologicalstandardization of salt-fermented fish product", J. KoreanSoc. Food Sci. Nutr, 25, pp.885~891 (1996)
- 전재호, "멸치 젓갈로부터 γ-Aminobutyric Acid(GABA)를 생성하는 Lactobacillus 속의 분리 · 동정", 한국식품영양학회지, 17(1), pp.72~79 (2004), 한국식품영양학회
- 정유정, "젓갈(염장발효식품)에서 분리한 호염세균의 생리 및 성장특성", 미생물학회지, 40(4), pp.263~268 (2004), 한국미생물학회
- Kim, H.-J., "Inhibition of spoilage and pathogenic bacteria bylacticin NK24, a bacteriocin produced by Lactococcus lactisNK24 from fermented fish food", Korean J. Food Sci.Technol, 31, pp.1035~1043 (1999)
- 김선재, "젓갈로부터 분리된 젖산균 및 효모의 프로바이오틱 특성", 한국식품저장유통학회지, 12(2), pp.184~189 (2005), 한국식품저장유통학회
- Lane,D.J, "16S-23S rRNA sequencing", pp.115~175 (1991), Nucleic AcidTechniques in Bacterial Systematics
- Lee,K.H, "Microbiological and enzymological studieson the flavor components of sea food pickles", J. KoreanAgric. Chem. Soc, 11, pp.1~6 (1969)
- Lee, J.-G, "Studies on the variation ofmicroflora during the fermentation of anchovy, Engraulisjaponica", Bull. Korean Fish. Soc, 7, pp.105~114 (1974)
- Lee,C.-H, "Fish fermentation technology", Korean J.Appl. Microbiol. Bioeng, 17, pp.645~654 (1989)
- Lee,W.D, "Recent development of jeotgal (traditionalKorean fermented seafood) and its future", Food Ind. Nutr, 6, pp.23~27 (2001)
- 이나경, "젓갈 프로바이오틱 생균의 내산성 및 내담즙 특성", 한국미생물·생명공학회지, 31(3), pp.297~300 (2003), 한국미생물·생명공학회
- Lee, J.-S., "Virgibacillus koreensis sp nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov with emended descriptions", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 56, pp.251~257 (2006), SOC GENERAL MICROBIOLOGY
- Levenga, H., "Fatal hemorrhagic pneumoniacaused by infection due to Kytococcus sedentarius-apathogen of passenger", Ann. Hematol, 83, pp.447~449 (2004)
- Longshaw, C. M., "Kytococcus sedentarius, the organism associatedwith pitted keratolysis, produces two keratin-degradingenzymes", J. Appl. Microbiol, 93, pp.810~816 (2002)
- Place, R. B., "Staphylococcus equorum subsp. linens, subsp. nov., astarter culture component for surface ripened semi-hardcheeses", Syst. Appl. Microbiol, 26, pp.30~37 (2003)
- Sands, A, "Microflora of fermentedKorean seafoods", J. Food Sci, 39, pp.1002~1005 (1974)
- Satomi, M., "Tetragenococcus muriaticus sp. nov., a new moderatelyhalophilic lactic acid bacterium isolated from fermentedsquid liver sauce", Int. J. Syst. Bacteriol, 47, pp.832~836 (1997)
- Sims, D., "Complete genome sequence of Kytococcus sedentarius typestrain (541T)", Stand. Genomic Sci, 1, pp.12~20 (2009)
- Spring, S., "Halobacillus gen. nov., with descriptionsof Halobacillus litoralis sp. nov. and Halobacillus sp. nov.,and transfer of Sporosarcina halophila to Halobacillushalophilus comb", nov. Int. J. Syst. Bacteriol, 46, pp.492~496 (1996)
- Um, M.-A, "Isolation and identificationof Staphylococcus sp. from Korean fermented fish products", J. Microbiol. Biotechnol, 6, pp.340~346 (1996)
- Yoon, J.-H., "Lentibacillussalicampi gen. nov., sp. nov., a moderately halophilic bacteriumisolated from a salt field in Korea", Int. J. Syst. Evol.Microbiol, 52, pp.2043~2048 (2002)
- Yoon, J.-H, "Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov.", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 54, pp.2163~2167 (2004), SOC GENERAL MICROBIOLOGY
- Yoon, J.-H., "Salimicrobium flavidum sp. nov., isolated from a marine solar saltern", Int. J. Syst. Evol. Microbiol, 59, pp.2839~2842 (2009)