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Characterization of the Thermophilic Bacterium Geobacillus sp. Strain GWE1 Isolated from a Sterilization Oven

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초록

A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between 60-80ºC (optimum 70ºC), in a pH range of 3.0-8.0 (optimum pH70ºC 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso-15:0 (13.3%), 16:1(ω7) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA–DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.

Abstract

A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between 60-80ºC (optimum 70ºC), in a pH range of 3.0-8.0 (optimum pH70ºC 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso-15:0 (13.3%), 16:1(ω7) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA–DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.

참고문헌 (32)

  1. Banat I, "Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov", Int. J. Syst. Evol. Microbiol, 54, pp.2197~2201 (2004)
  2. Bergey DH, "Bergey's Manual of Determinative Bacteriology" (1994), Lippincott Williams & Wilkins
  3. Chen XG, "Thermoactive extracellular proteases of Geobacillus caldoproteolyticus, sp. nov., from sewage sludge", Extremophiles, 8, pp.489~498 (2004)
  4. Coorevits A, "Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly ‘thermoglucosidasius’); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov", Int. J. Syst. Evol. Microbiol, 62, pp.1470~1485 (2012)
  5. Cowan ST, "Identification of Medical Bacteria. Appx. D", pp.146~165 (1965), University Press
  6. De Ley J, "The quantitative measurement of DNA hybridization from renaturation rates", Eur. J. Biochem, 12, pp.133~142 (1970)
  7. Derekova A, "Biosynthesis of a thermostable gellan lyase by newly isolated and characterized strain of Geobacillus stearothermophilus 98", Extremophiles, 10, pp.321~326 (2006)
  8. Felsenstein J., "Confidence limits on phylogenies: An approach using the bootstrap", Evolution, 39, pp.783~791 (1985)
  9. Fortina MG, "Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov", Int. J. Syst. Evol. Microbiol, 51, pp.2063~2071 (2001)
  10. Higgins DG, "Clustal:a package for performing muliple sequence alignments on a microcomputer", Gene, 73, pp.237~244 (1988)
  11. Huss VAR, "Studies on the spectrophotometric determination of DNA hybridization from renaturation rates", Syst. Appl. Microbiol, 4, pp.184~192 (1983)
  12. Johnson JL, "Genetic characterization, pp, In Manual of methods for general bacteriology", pp.450~472 (1981), ASM
  13. Lanyi B., "Classical and rapid identification methods for medically important bacteria", Methods Microbiol, 19, pp.1~67 (1987)
  14. Li H, "Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1", Protein Expr. 10Purif, 42, pp.153~159 (2005)
  15. Marchant R, "What are high temperature bacteria doing in cold environments?", Trends Microbiol, 10, pp.120~121 (2002)
  16. Marchant R, "The frequency and characteristics of highly thermophilic bacteria in cool soil environments", Environ. Microbiol, 4, pp.595~602 (2002)
  17. Metin K, "Characterization of esterase activity in Geobacillus sp. HBB-4", J. Basic Microbiol, 46, pp.400~409 (2006)
  18. Mesbah M, "Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography", Int. J. Syst. Bact, 39, pp.159~167 (1989)
  19. Nazina TN, "Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans", Int. J. Syst. Evol. Microbiol, 51, pp.33~446 (2001)
  20. Nordström KM, "Effect of growth temperature on fatty acid composition of ten Thermus strains", Appl. Environ. Microbiol, 58, pp.1656~1660 (1992)
  21. Reysenbacha AL, "Microbiology of ancient and modern hydrothermal systems", Trends Microbiol, 9, pp.79~86 (2001)
  22. Rudi K, "Strain classification of oxyphotobacteria in clone cultures on the basis of 16S rRNA sequences from variable regions V6, V7 and V8", Appl. Environ. Microbiol, 63, pp.2593~2599 (1997)
  23. Saitou N, "The neighbor-joining method: A new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
  24. Tai SK, "Isolation and characterization of a cellulolytic Geobacillus thermoleovorans T4 strain from sugar refinery wastewater", Extremophiles, 8, pp.345~349 (2006)
  25. Solimam, Nadia A, "Coproduction of Thermostable Amylase and β-Galactosidase Enzymes by Geobacillus stearothermophilus SAB-40: Application of Plackett-Burman Design", Journal of Microbiology and Biotechnology, 18(4), pp.695~703 (2008), 한국미생물·생명공학회
  26. Tamura K, "Prospects for inferring very large phylogenies by using the neighbor-joining method", PNAS (USA), 101, pp.11030~11035 (2004)
  27. Tamura K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0", Mol. Biol. Evol, 24, pp.1596~1599 (2007)
  28. Tanner MA, "Complex microbial communities inhabiting sulfide-rich black mud from marine coastal environments", Biotechnology et alia, 8, pp.1~16 (2000)
  29. Thompson JC, "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position, specific gap penalties, and weight matrix choice", Nucleic Acids Res, 22, pp.4673~4680 (1994)
  30. Watanabe K, "Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting", J. Microb. Method, 44, pp.253~262 (2001)
  31. Zhang Y, "A moderately thermostable alkaline phosphatase from Geobacillus thermodenitrificans T2: cloning, expression and biochemical characterization", Appl. Biochem. Biotechnol, 151, pp.81~92 (2008)
  32. Zhang Y, "Cloning, expression, and characterization of a thermostable PAP2L2, a new member of the type-2 phosphatidic acid phosphatase family from Geobacillus toebii T-85", Biosci. Biotechnol. Biochem, 72, pp.3134~3141 (2008)

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