수소 생산과 세균 군집구조에 미치는 PVA-포괄고정화의 영향
Effect of PVA-Encapsulation on Hydrogen Production and Bacterial Community Structure
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In this study, the performances of PVA-encapsulation and non-encapsulation in a fed-batch bioreactor system were comparedfor biohydrogen production. Hydrogen production in the PVA-encapsulation bioreactor was not significantly different in comparisonto the non-encapsulation bioreactor. However, the hydrogen gas in the encapsulation bioreactor could be stably producedwhen it was exposed to environmental difficulties such as pH impact by the accumulation of organic acids as fermentative metabolicproducts. Bacterial communities by DGGE analysis were differently shifted between the PVA-encapsulation and nonencapsulationbioreactors from the initial sludge. The community of hydrogen producing bacteria was stable during the experimentalperiod in the PVA-encapsulation bioreactor compared to the non-encapsulation method. The absolute quantitation ofthe DNA copy number by a high-throughput droplet digital PCR system for six genera contributed to hydrogen productionshowing that the numbers of dominant bacteria existed at similar levels in the two bioreactors regardless of encapsulation. Inboth of two bioreactors, not only Clostridium and Enterobacter, which are known as anaerobic hydrogen producing bacteria,but also Firmicutes, Ruminococcus and Escherichia existed with 1 × 10⁵-1 × 10⁶ copy numbers of ml-samples exhibiting rapidgrowth during the initial operation period.
Abstract
In this study, the performances of PVA-encapsulation and non-encapsulation in a fed-batch bioreactor system were comparedfor biohydrogen production. Hydrogen production in the PVA-encapsulation bioreactor was not significantly different in comparisonto the non-encapsulation bioreactor. However, the hydrogen gas in the encapsulation bioreactor could be stably producedwhen it was exposed to environmental difficulties such as pH impact by the accumulation of organic acids as fermentative metabolicproducts. Bacterial communities by DGGE analysis were differently shifted between the PVA-encapsulation and nonencapsulationbioreactors from the initial sludge. The community of hydrogen producing bacteria was stable during the experimentalperiod in the PVA-encapsulation bioreactor compared to the non-encapsulation method. The absolute quantitation ofthe DNA copy number by a high-throughput droplet digital PCR system for six genera contributed to hydrogen productionshowing that the numbers of dominant bacteria existed at similar levels in the two bioreactors regardless of encapsulation. Inboth of two bioreactors, not only Clostridium and Enterobacter, which are known as anaerobic hydrogen producing bacteria,but also Firmicutes, Ruminococcus and Escherichia existed with 1 × 10⁵-1 × 10⁶ copy numbers of ml-samples exhibiting rapidgrowth during the initial operation period.
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