백두구 추출물의 항산화 및 항비만 효과
Anti-Oxidative and Anti-Obesity Effects of Amomum Cardamomum L. Extract
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In this study, the anti-oxidative and anti-obesity activities of Amomum cardamomum L. methanol extract (ACME) were evaluated using DPPH radical scavenging activity assay, pancreatic lipase enzyme inhibition assay, and the cell culture model system. ACME exhibited DPPH radical scavenging activities dose-dependently, with IC50 of DPPH radical scavenging activities of ACME being 25.15 μg/ml. Furthermore, ACME effectively suppressed pancreatic lipase enzyme activity dose-dependently. ACME also significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents, and triggered lipolysis activity on 3T3-L1 preadipocytes in a dose-dependent manner, without cytotoxicity. Their anti-obesity effect was modulated by the cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins α (C/EBPα), C/EBPβ and the peroxisome proliferator-activated receptor γ (PPARγ) gene and protein expressions. Taken together, these results provide an important new insight that A. cardamomum L. possesses anti-oxidative and anti-obesity activities such as pancreatic lipase inhibition, anti-adipogenic, and lipolysis effects. There is therefore potential for its use as a promising component in the field of nutraceuticals and the identification of the active compounds that confer the anti-oxidative and anti-obesity activities of ACME might be an appropriate next step.
Abstract
In this study, the anti-oxidative and anti-obesity activities of Amomum cardamomum L. methanol extract (ACME) were evaluated using DPPH radical scavenging activity assay, pancreatic lipase enzyme inhibition assay, and the cell culture model system. ACME exhibited DPPH radical scavenging activities dose-dependently, with IC50 of DPPH radical scavenging activities of ACME being 25.15 μg/ml. Furthermore, ACME effectively suppressed pancreatic lipase enzyme activity dose-dependently. ACME also significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents, and triggered lipolysis activity on 3T3-L1 preadipocytes in a dose-dependent manner, without cytotoxicity. Their anti-obesity effect was modulated by the cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins α (C/EBPα), C/EBPβ and the peroxisome proliferator-activated receptor γ (PPARγ) gene and protein expressions. Taken together, these results provide an important new insight that A. cardamomum L. possesses anti-oxidative and anti-obesity activities such as pancreatic lipase inhibition, anti-adipogenic, and lipolysis effects. There is therefore potential for its use as a promising component in the field of nutraceuticals and the identification of the active compounds that confer the anti-oxidative and anti-obesity activities of ACME might be an appropriate next step.
참고문헌 (30)
- Acharya A, "Chemopreventive properties of indole-3-carbinol, diindolylmethane and other constituents of cardamom against carcinogenesis", Recent Pat. Food Nutr. Agric, 2, pp.166~177 (2010)
- Attie AD, "Adipocyte metabolism and obesity", J. Lipid Res, 50, pp.395~399 (2009)
- 배초롱, "식이유도 비만 Mice에서 된장 및 무염된장의 항비만 효과", 한국식품영양과학회지, 42(7), pp.1036~1042 (2013), 한국식품영양과학회
- Cao Z, "Regulcated expresstion of three C", EBP isoforms during adipose conversion of 3T3-L1 cells, 1991, pp.5, 1538~1552
- Chen HC, "Inhibition of triglyceride synthesis as a treatment strategy for obesity: lessons from DGAT1-deficient mice", Arterioscler. Thromb. Vasc. Biol, 25, pp.482~486 (2005)
- Chen Z, "Krox20 stimulates adipogenesis via C", EBPbeta-dependent and –independent mechanisms, 2005, pp.1, 93~106
- Chua SC Jr, "Monogenic models of obesity", Behav. Genet, 27, pp.277~284 (1997)
- Després JP, "Abdominal obesity and metabolic syndrome", Nature, 444, pp.881~887 (2006)
- Flier JS, "Obesity and the hypothalamus:novel peptides for new pathways", Cell, 92, pp.437~440 (1998)
- Gesta S, "Developmental origin of fat: Tracking obesity to its source", Cell, 131, pp.242~256 (2007)
- Gregoire FM, "Understanding adipocyte differentiation", Physiol. Rev, 78, pp.783~809 (1998)
- Gupta R, "Lipase assays for conventional and molecular screening: an overview", Biotechnol. Appl. Biochem, 37, pp.63~71 (2003)
- Heck AM, "Orlistat, a new lipase inhibitor for the management of obesity", Pharmacotherapy, 20, pp.270~279 (2000)
- Hotamisligil GS, "Inflammation and metabolic disorders", Nature, 444, pp.860~867 (2006)
- Kedare SB, "Genesis and development of DPPH method of antioxidant assay", J. Food Sci. Technol, 48, pp.412~422 (2011)
- 김소혜, "비만성인의 영양소 섭취량 및 식사 다양성 평가", 대한지역사회영양학회지, 12(5), pp.583~591 (2007), 대한지역사회영양학회
- 김은정, "고지방 식이로 유도된 비만 백서에서 뽕잎 추출물의 항비만 효과", 동의생리병리학회지, 23(4), pp.831~836 (2009), 대한동의병리학회
- Lee DH, "Detection of antiinflammatory agents from natural products as inhibitors of cyclooxygenase I and II", Nat. Prod. Sci, 3, pp.19~28 (1997)
- Liu F, "An extract of Lagerstroemia speciosa L. has insulin-like uptake-stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1cells", J. Nutr, 131, pp.2242~2247 (2001)
- Amin F. Majdalawieh, "In Vitro Investigation of the Potential Immunomodulatory and Anti-Cancer Activities of Black Pepper (Piper nigrum) and Cardamom (Elettaria cardamomum)", Journal of Medicinal Food, 13(2), pp.371~381 (2010), 한국식품영양과학회
- Morrison RF, "Hormonal signaling and transcriptional control of adipocyte differentiation", J. Nutr, 130, pp.3116~3121 (2000)
- Ntambi JM, "Adipocyte differentiation and gene expression", J. Nutr, 130, pp.3122~3126 (2000)
- 박정애, "흑마늘 추출물에 의한 3T3-L1 지방전구세포의 분화 및 adipogenesis 억제에 관한 연구", 생명과학회지, 21(5), pp.720~728 (2011), 한국생명과학회
- Rosen ED, "Adipocyte differentiation from the inside out", Nat. Rev. Mol. Cell Biol, 7, pp.885~896 (2006)
- Suzuki R, "Anthocyanidins-enriched bilberry extracts inhibit 3T3-L1 adipocyte differentiation via the insulin pathway", Nutr. Metab. (Lond), 8, pp.8~14 (2011)
- Vogel C, "Insights into the regulation of protein abundance from proteomic and transcriptomic analyses", Nat. Rev. Genet, 13, pp.227~232 (2012)
- Wu Z, "Indeuction of peroxisome proliferator-activated receptor gamma during the conversion of 3T3 fibroblasts into adipocytes is mediated by C", EBP beta, C, Mol. Cell Biol, pp.1996, 16[DOI]
- Wu Z, "Conditional etopic expression of C", EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis, 1995, pp.9, 2350~2363
- Yeh WC, "Cascade regulation of terminal adipocyte differentiation by three members of the C", EBP family of leucine zipper proteins, 1995, pp.9, 168~181
- Zhang JW, "Role of CREB in transcriptional regulation of CCAAT", enhancer-binding protein beta gene during adipogenesis, 2004, pp.279, 4471~4478