환경 유형에 따른 바이오에어로졸 중 배양성 세균 동정 및 계통분석
Identification and Phylogenetic Analysis of Culturable Bacteria in the Bioareosol from Several Environments
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초록
바이오에어로졸은 0.02−100 μm 입자로, 자연 및 인위적 환경 또는 사람의 활동에 의해 발생된다. 바이오에어로졸은 바이러스, 세균, 곰팡이, 원생생물, 곰팡이 포자, 미생물 독소, 꽃가루, 동식물 기원의 물질, 타액 또는 글루칸 등으로 구성되며, 인간과 동물에게 호흡기 등의 질환을 유발한다. 본연구에서는 농업, 축산, 하수처리장, 해변 및 청청지역의 바이오에어로졸 시료에서 분리한 배양성 미생물을 동정 및 계통분석을 수행한 결과, 우점 및 종의 구성 등 환경 유형 별 차이가 분석되었다. 한편 모든 시료에서 31 분리주가 Bacillus cereus 로 동정되었고, 실내 양계장에서 Acinetobacter baumannii가 분리되었다. 또한 농업, 축산 및 해변에서 분리한 미생물 중Domibacillus속, Chryceobacterium속, Nocardioides속 및 Comamonadaceae과에 속하는 새로운 종 또는 속이 분리되었다.
Abstract
Bioaerosols are comprised of particles 0.02−100 μm in size that originate in natural and artificial environments, and as a result of human activities. They consist of microorganisms including viruses, bacteria, fungi, and protozoa; fungal spores; microbial toxins; pollen; plant or animal material; expectorated liquid from humans; and glucans (peptidoglycan and β-glucan). Bioaerosols can cause respiratory and other diseases in humans and animals. In this study, bioaerosol samples acquired from agricultural sources, livestock, a sewage treatment plant, a beach, and a pristine area were analyzed to identify and phylogenetically characterize culturable microorganisms. The isolated bacteria exhibited regional differences, with different species dominating. However, Bacillus cereus was isolated in all samples, with a total of 31 strains isolated from all areas, and Acinetobacter baumannii was isolated from an indoor poultry farm. In addition, bacteria determined to be of novel genus or species of the genera Domibacillus, Chryceobacterium, Nocardioides and family Comamonadaceae were isolated from the agricultural, livestock and beach environments.
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