Characteristics of Morphological and Physiological Changes during the Autolysis Process of Saccharomyces cerevisiae FX-2
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In this paper, the autolysis process of Saccharomyces cerevisiae FX-2 (S. cerevisiae FX-2) via, a variety of endogenous enzyme, was investigated systematically by analyzing changes in physicochemical parameters in autolysate, surface morphology and the internal structure of the yeast cells. As an explicit conclusion, the arisen autolysis depended on the pH and the optimal pH was found to be 5.5. Based on the experimental data and the characteristics of mycelia morphology, a hypothesis is put forward that simple proteins in yeast vacuolar are firstly degraded for utilization, and then more membrane- bound proteins are hydrolyzed to release hydrolytic enzymes, which arouse an enzymatic reaction to induce the collapse of the cell wall into the cytoplasm.
Abstract
In this paper, the autolysis process of Saccharomyces cerevisiae FX-2 (S. cerevisiae FX-2) via, a variety of endogenous enzyme, was investigated systematically by analyzing changes in physicochemical parameters in autolysate, surface morphology and the internal structure of the yeast cells. As an explicit conclusion, the arisen autolysis depended on the pH and the optimal pH was found to be 5.5. Based on the experimental data and the characteristics of mycelia morphology, a hypothesis is put forward that simple proteins in yeast vacuolar are firstly degraded for utilization, and then more membrane- bound proteins are hydrolyzed to release hydrolytic enzymes, which arouse an enzymatic reaction to induce the collapse of the cell wall into the cytoplasm.
참고문헌 (27)
- Cui C, "Review o n the difference between HVP and EVP", China Condiment, 329, pp.9~13 (2006)
- Dziezak JD, "Yeast and yeast derivatives: applications", Food Technol, 41, pp.122~125 (1987)
- Jacobs MB, "Micro-Kjeldahl method for biological", J. Am. Pharm. Assoc, 40, pp.151~153 (2006)
- Johnson JC., "Yeast for Food and Other Purposes", pp.158~177 (1977), Noyes Data Corporation
- Halasz A, "Use of yeast biomass in food production", pp.172~195 (1991), CRC Press
- Hernawan T, "Chemical and cytological changes during the autolysis of yeasts", J. Ind. Microbiol, 14, pp.440~450 (1995)
- Hawk PB, "Practical Physiological Chemistry" (1976), McGraw-Hill Book Co., Inc
- Huai XJ, "The impact on digestive function of Litopenaeus vannamei yeast extract replace fish meal", Fisheries Science & Technology Information, 40, pp.240~244 (2013)
- Knorr D, "Enzymatic lysis of cell walls", Biotechnol. Bioeng, 21, pp.2011~2021 (1979)
- Li X, "Study onYeast autolysis", J. China Brewing, 15, pp.13~17 (2001)
- Liu XJ, "Research progress in Saccaramyces cerevisiae vacuolar proteinase", China Food Sci. Technol, 31, pp.377~379 (2010)
- Martínez-Rodríguez AJ, "Structural and ultrastructural changes in yeast cells during autolysis in a model wine system and in sparkling wines", Int. J. Food Microbiol, 75, pp.45~51 (2001)
- Milic TV, "Utilization of baker’s yeast (Saccharomyces cerevisiae) for the production of yeast extract: effects of different enzymatic treatments on solid, protein and carbohydrate recovery", J. Serb. Chem. Soc, 72, pp.451~457 (2007)
- Morya VK, "Production and characterization Te-Peptide by induced autolysis of Saccharomyces Cerevisiae", Appl. Biochem. Biotechnol, 172, pp.3390~3401 (2014)
- Margit DA, "Chemical and sensory characterization of hydrolyzed vegetable protein, a savory flavoring", J. Agr. Food Chem, 46, pp.481~489 (1998)
- Nagodawithana T, "Yeast-derived flavors and flavor enhancers and their probable mode of action", Food Technol, 46, pp.138~140 (1992)
- Oliveira AM, "Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3hydrolysis", Braz. Arch. Biol. Techn, 54, pp.1007~1018 (2011)
- Roman K, "Induction and acceleration of yeast lysis by addition of fresh yeast autolysis", Biotechnol. Lett, 13, pp.543~546 (1991)
- Rainina EI, "A cytochemical study of the localization of acid phosphatase insaccharomyces cerevisiae at different growth phases", Histochem. J, 11, pp.299~310 (1979)
- Sugimoto H, "Synergistic effect of ethanol and sodium chlo-ride on autolysis of baker’s yeast for preparing foodgrade yeast extracts", J. Food Sci, 39, pp.939~944 (1974)
- Slaughter JC, "Autocatalytic degradation of proteins in extracts of a brewing strain of Saccharomyces cerevisiae. The role of endoproteinases and exopeptidases", Appl. Microbiol. Biotechnol, 37, pp.638~642 (1992)
- Torresi S, "Effects of a β-glucanase enzymatic preparation on yeast lysis during aging of traditional sparkling wines", Food Res. Int, 55, pp.83~92 (2014)
- Van Den Hazel HB, "Review: biosynthesis and function of yeast vacuolar proteases", Yeast, 12, pp.1~16 (1996)
- Verduyn C, "Effect of high pressure homogenization and papain on the preparation of autolysed yeast extract", World J. Microb. Biot, 15, pp.57~63 (1999)
- Yu SC, "Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87", Cancer Lett, 265, pp.124~134 (2008)
- Zhao J, "Degradation of RNA during the autolysis of Saccharomyces cerevisiaeproduces predominantly ribonucleotides", J. Ind. Microbiol. Biot, 32, pp.415~423 (2005)
- Zu M, "Study on the enzymatic process of high protein cotent of baker’s yeast", Tianjing Agr. Sci, 19, pp.37~41 (2013)