화살나무(Euonymus alatus)로 부터 α-glucosidase 저해 물질의 분리 및 동정
Isolation and Characterization of α-glucosidase Inhibitors from Euonymus alatus
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초록
화살나무 가지로부터 activity based fractionation에 의해α-glucosidase 저해 활성 물질 compound 1−4을 분리하였고, 1H NMR, 13C NMR, 1H−1H COSY와 HMBC 등의 spectral data에 의해 구조를 결정하였다. Compound 1−4는 모두flavonol 물질로 α-glucosidase에 대해 IC50 값이 각각 25.3, 17.1, 47.3과 35.1 μM로 positive control로 사용한 acarbose 보다 강한 활성을 나타내었다. 화살나무의 혈당 저하 기능의유효성분으로 처음 동정된 Compound 1−4는 α-glucosidase 에만 특이적 활성을 갖는 물질로 당뇨병 치료제로의 개발 가능성이 높은 물질로 사료된다.
Abstract
α-glucosidase inhibitory compounds (1−4) in a 50% methanol extract of Euonymus alatus were isolated by activity-based fractionations and the structures determined on the basis of chemical and spectral characterization techniques such as 1H and 13C nuclear magnetic resonance spectroscopy, 1H− 1H correlation spectroscopy (COSY), and heteronuclear multiple bond correlation (HMBC). The compounds 1−4 belong to flavonols and exhibited potent inhibitory activities against α-glucosidase, with IC50 values of 25.3, 17.1, 47.3, and 35.1 μM, respectively. All the isolated compounds were more potent than the positive control acarbose. This is the first report describing the potential hypoglycemic effect of Euonymus alatus through α-glucosidase inhibition and identification of its active components.
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