Lipase-producing Filamentous Fungi from Non-dairy Creamer Industrial Waste
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Lipase-producing fungi have been isolated from environments containing lipids. The non-dairy creamer industrial waste has a high amount of lipids so it is a potential source for the isolation of lipase-producing fungi. However, the study of fungi that secrete lipase from this industrial waste has not been reported. The purpose of this study was to obtain lipase-producing filamentous fungi from non-dairy creamer industrial waste. Mineral salt and potato dextrose agar were used as media for the isolation process. The qualitative screening was conducted using phenol red agar medium and the quantitative screening using broth medium containing glucose and olive oil. Isolates producing the highest amounts of lipase were identified with molecular methods. We found that 5 out of 19 isolated filamentous fungi are lipase producers. Further analysis showed that isolate Ms.11 produced the highest amount of lipase compared to others. Based on ITS sequence Ms.11 was identified as Aspergillus aculeatus. The lipase activity in medium containing 1% glucose + 1% olive oil at pH 7.0 and 30℃ after 96 and 120 h of incubation was 5.13 ± 0.30 U/ml and 5.22 ± 0.59 U/ml, respectively. The optimum lipase activity was found at pH 7.0, 30℃ and using methanol or ethanol in the reaction tube. Lipase was more stable at 20−30℃ and maintained 85% of its activity. It was concluded that isolate Ms.11 is a potential source of lipase that catalyzes transesterification reactions. Further studies are required to optimize lipase production to make the strain suitable for industry purposes.
Abstract
Lipase-producing fungi have been isolated from environments containing lipids. The non-dairy creamer industrial waste has a high amount of lipids so it is a potential source for the isolation of lipase-producing fungi. However, the study of fungi that secrete lipase from this industrial waste has not been reported. The purpose of this study was to obtain lipase-producing filamentous fungi from non-dairy creamer industrial waste. Mineral salt and potato dextrose agar were used as media for the isolation process. The qualitative screening was conducted using phenol red agar medium and the quantitative screening using broth medium containing glucose and olive oil. Isolates producing the highest amounts of lipase were identified with molecular methods. We found that 5 out of 19 isolated filamentous fungi are lipase producers. Further analysis showed that isolate Ms.11 produced the highest amount of lipase compared to others. Based on ITS sequence Ms.11 was identified as Aspergillus aculeatus. The lipase activity in medium containing 1% glucose + 1% olive oil at pH 7.0 and 30℃ after 96 and 120 h of incubation was 5.13 ± 0.30 U/ml and 5.22 ± 0.59 U/ml, respectively. The optimum lipase activity was found at pH 7.0, 30℃ and using methanol or ethanol in the reaction tube. Lipase was more stable at 20−30℃ and maintained 85% of its activity. It was concluded that isolate Ms.11 is a potential source of lipase that catalyzes transesterification reactions. Further studies are required to optimize lipase production to make the strain suitable for industry purposes.
참고문헌 (51)
- Abdelmonaem M, "Classification of EC 3.1.1.3 bacterial true lipases using phylogenetic analysis", Afr. J. Biotechnol, 9, pp.8243~8247 (2010)
- Singh AK, "Overview of fungal lipase : A review", Appl. Biochem. Biotechnol, 166, pp.486~520 (2012)
- Ponnarasy G, "Light induced esterification of oleic acid catalyzed by Pseudomonas Cepacia Lipase", Int. J. Environ. Sci. Dev, 5, pp.344~346 (2014)
- Hassan MHj, "An Overview of biofuel as a renewable energy source : development and challenges", Procedia Eng, 56, pp.39~53 (2013)
- Gupta R, "Bacterial lipases : an overview of production, purification and biochemical properties", Appl. Microbiol. Biotechnol, 64, pp.763~781 (2004)
- Shreya, "Isolation and screening of lipolytic soil fungi", Int. J. Pharm. Biol. Sci, 8, pp.391~396 (2018)
- Berto P, "Production and purification of a novel extracellular lipase from Alternaria brassicicola", Biotechnol. Lett, 19, pp.533~536 (1997)
- Gopinath SCB, "Extracellular enzymatic activity profiles in fungi isolated from oil-rich environments", Mycoscience, 46, pp.119~126 (2005)
- Sadati R, "Isolation and screening of lipolytic fungi from coastal waters of the southern caspian sea(North of Iran)", Jundishapur J. Microbiol, 8, pp.1~7 (2015)
- Rihani A, "Isolation and identification of lipase-producing fungi from local olive oil manufacture in East of Algeria", Chem Chem. Eng. Biotechnol. Food Ind, 19, pp.13~22 (2018)
- Nwuche CO, "Isolation of lipase producing fungi from palm oil Mill effluent(POME)dump sites at Nsukka", Braz. Arch Biol. Technol, 54, pp.113~116 (2011)
- Sharma R, "Production, purification, characterization, and applications of lipases", Biotechnol. Adv, 19, pp.627~662 (2001)
- Rosida DF, "A comparative study of non-dairy cream based on the type of leguminosae protein source in terms of physico chemical properties and organoleptic", Agric. Agric. Sci. Procedia, 9, pp.431~439 (2016)
- Lee LP, "Lipase-Secreting Bacillus Species in an oil-contaminated habitat : promising strains to alleviate oil pollution", BioMed. Res. Int, 2015, pp.1~9 (2015)
- Gohel V, "Bioprospecting and antifungal potential of chitinolytic microorganisms", Afr. J. Biotechnol, 5, pp.54~72 (2006)
- Adham NZ, "Extracellular lipase of Aspergillus niger NRRL3; production, partial purification and properties", Indian J. Microbiol, 49, pp.77~83 (2009)
- Lima LGR, "Lipase production by Aspergillus niger C by submerged fermentation", Braz. Arch Biol Technol, 62, pp.1~14 (2019)
- Kwon DY, "A simple and rapid colorimetric method for determination of free fatty acids for lipase assay", J. Am. Oil Chem. Soc, 63, pp.89~92 (1986)
- Ilmi M, "Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62", Biocatal. Agric. Biotechnol, 9, pp.103~107 (2017)
- Arya A, "Understanding Enzymes: An Introductory Text" (2018), Drawing Pin Publishing
- Lowry OH, "Protein measurement with the Folin phenol reagent", J. Biol. Chem, 193, pp.265~276 (1951)
- Raja HA, "Fungal identification using molecular tools : A primer for the natural products research community", J. Nat. Prod, 80, pp.756~770 (2017)
- Mahmoud AGY, "Why nuclear ribosomal internal transcribed spacer (ITS) has been selected as the DNA barcode for fungi?", Adv. Genet. Eng, 04, pp.1~2 (2015)
- Begerow D, "Current state and perspectives of fungal DNA barcoding and rapid identification procedures", Appl. Microbiol. Biotechnol, 87, pp.99~108 (2010)
- Silva DM, "Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy", Braz. J. Microbiol, 42, pp.761~773 (2011)
- Molina G, "New and Future Developments in Microbial Biotechnology and Bioengineering", pp.155~169 (2016)
- Yin Z, "Enhancement of maize growth and alteration of the rhizosphere microbial community by phosphate-solubilizing fungus Aspergillus aculeatus P93", J. Agric. Biotechnol, 2, pp.1~10 (2017)
- de Oliveira RL, "Thermodynamic and kinetic studies on pectinase extracted from Aspergillus aculeatus : Free and immobilized enzyme entrapped in alginate beads", Int. J. Biol. Macromol, 115, pp.1088~1093 (2018)
- Surisa Suwannarangsee, "Production and Characterization of Multi-Polysaccharide Degrading Enzymes from Aspergillus aculeatus BCC199 for Saccharification of Agricultural Residues", Journal of Microbiology and Biotechnology, 24(10), pp.1427~1437 (2014), 한국미생물·생명공학회[DOI]
- Boonmee A, "Hydrolysis of various thai agricultural biomasses using the crude enzyme from Aspergillus aculeatus iizuka FR60 isolated from soil", Braz. J. Microbiol, 43, pp.456~466 (2012)
- Roy M, "Identification of lipase producing fungus isolated from dairy waste contaminated soil and optimization of culture conditions for lipase production by the isolated fungus", J. Microbiol. Biotechnol. Food Sci, 8, pp.698~704 (2018)
- Armas JC, "Mucor griseocyanus lipase : production, characterization and study of some catalytic properties of the Immobilized Enzyme", Food Technol. Biotechnol, 46, pp.195~201 (2008)
- Silva OBW, "Production and extraction of an extracellular lipase from the entomopathogenic fungus Metarhizium anisopliae", Process Biochem, 40, pp.321~326 (2005)
- El-Ghonemy DH, "Extracellular alkaline lipase from a novel fungus Curvularia sp. DHE 5:optimization of physicochemical parameters, partial purification and characterization", Food Technol. Biotechnol, 55, pp.206~217 (2017)
- Rajeswari T, "Biosynthesis of novel alkaline lipase production from Penicillum Chrysogenum suitable for detergent formulation", Res. J. Pharm. Biol. Chem. Sci, 2, pp.128~141 (2011)
- Rajan A, "A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657in submerged and solid-state fermentation using economically and industrially feasible substrate", Turk. J. Biol, 569(574) (2011)
- Salihu A, "Production and applications of microbial lipases : A review", Sci. Res. Essays, 7, pp.2667~2777 (2012)
- Ayinla ZA, "Studies on the optimization of lipase production by Rhizopus sp. ZAC3 isolated from the contaminated soil of a palm oil processing shed", J. Appl. Biol. Biotechnol, 5, pp.30~37 (2017)
- Wang X, "Growth strategy of microbes on mixed carbon sources", Nat. Commun, 10, pp.1279~ (2019)
- Chu D, "The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate", Sci. Rep, 6, pp.25191~ (2016)
- Robinson PK, "Enzymes : principles and biotechnological applications", Essays Biochem, 59, pp.1~41 (2015)
- Ibrahim CO, "Purification and some properties of a thermostable lipase from Humicola lanuginosa No. 3", Agric. Biol. Chem, 51, pp.37~45 (1987)
- Falony G, "Production of extracellular lipase from Aspergillus niger by solid-state fermentation", Food Technol. Biotechnol, 44, pp.235~240 (2006)
- Mahadik ND, "Production of acidic lipase by Aspergillus niger in solid state fermentation", Process Biochem, 38, pp.715~721 (2002)
- Kishore D, "Thermal, chemical and pH induced denaturation of a multimeric β-Galactosidase reveals multiple unfolding pathways", PLoS One, 7, pp.e50380~ (2012)
- Pera LM, "Catalytic properties of lipase extracts from Aspergillus niger", Food Technol. Biotechnol, 44, pp.247~252 (2006)
- Rodrigues RC, "Enzymatic synthesis of biodiesel from transesterification reactions of vegetable oils and short chain alcohols", J. Am. Oil Chem. Soc, 85, pp.925~930 (2008)
- Bernardes OL, "Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase", Appl. Biochem. Biotechnol, 7, pp.105~114 (2007)
- Lotti M, "Effects of methanol on lipases : Molecular, kinetic and process issues in the production of biodiesel", Biotechnol. J, 10, pp.22~30 (2015)
- Herkovits TT, "On the structural stability and solvent denaturation of proteins", J. Biol. Chem, 245, pp.2588~2598 (1970)
- Romero CM, "Purification of an organic solvent-tolerant lipase from Aspergillus niger MYA 135 and its application in ester synthesis", Biocatal. Agric. Biotechnol, 1, pp.25~31 (2012)