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Fitness cost and competitive ability of transgenic herbicide-tolerant rice expressing a protoporphyrinogen oxidase gene

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초록

The expression of transgenic traits in genetically modified crops is sometimes associated with decreases in crop performance or fitness. These decreases in performance or fitness of transgenic plants in unfavourable conditions may provide valuable information about the ecological consequences of transgene escape. In a glasshouse trial, we tested the cost associated with resistance to herbicides by comparing the growth, yield, and competitive ability of transgenic rice with its parental non-transgenic line. This new line was developed for constitutive overexpression of protoporphyrinogen oxidase (PPO) to increase resistance to herbicides. We evaluated nine agronomic traits of transgenic and non-transgenic rice grown in a replacement series design over four densities. Competitive ability was also assessed between transgenic and non-transgenic plants by analyzing their relative yields based on biomass and seed weight data. Our results indicated that non-transgenic plants showed greater performance than did the transgenic plants when those genotypes were grown in mixtures. The non-transgenic rice plants exhibited superior competitive ability at certain combinations of planting densities and genotype proportions. These results suggest that PPO-herbicide resistance incurs some costs in plant performance and competitive ability.

Abstract

The expression of transgenic traits in genetically modified crops is sometimes associated with decreases in crop performance or fitness. These decreases in performance or fitness of transgenic plants in unfavourable conditions may provide valuable information about the ecological consequences of transgene escape. In a glasshouse trial, we tested the cost associated with resistance to herbicides by comparing the growth, yield, and competitive ability of transgenic rice with its parental non-transgenic line. This new line was developed for constitutive overexpression of protoporphyrinogen oxidase (PPO) to increase resistance to herbicides. We evaluated nine agronomic traits of transgenic and non-transgenic rice grown in a replacement series design over four densities. Competitive ability was also assessed between transgenic and non-transgenic plants by analyzing their relative yields based on biomass and seed weight data. Our results indicated that non-transgenic plants showed greater performance than did the transgenic plants when those genotypes were grown in mixtures. The non-transgenic rice plants exhibited superior competitive ability at certain combinations of planting densities and genotype proportions. These results suggest that PPO-herbicide resistance incurs some costs in plant performance and competitive ability.

참고문헌 (53)

  1. Bartsch DI, "Competitiveness of transgenic sugar beet resistant to beet necrotic yellow vein virus and potential impact on wild beet populations", Mol Ecol, 5, pp.199~205 (1996)
  2. Bartsch D, "Biosafety of hybrids between transgenic virus-resistant sugar beet and Swiss chard", Ecol Appl, 11, pp.142~147 (2001)
  3. Bergelson J, "Surveying patterns in the cost of resistance in plants", Am Nat, 148, pp.536~558 (1996)
  4. Bergelson J, "Costs of resistance : a test using transgenic Arabidopsis thaliana", P Roy Soc Lond B Bio, 263, pp.1659~1663 (1996)
  5. Brandle JE, "Agronomic performance of sulfonylurea-resistant transgenic flue-cured tobacco grown under field conditions", Crop Sci, 33, pp.847~852 (1993)
  6. Burke JM, "Fitness effects of transgenic disease resistance in sunflowers.", Science, 300, pp.1250~ (2003)
  7. Chapman MA, "Letting the gene out of bottle : the population genetics of genetically modified crops", New Phytol, 170, pp.429~443 (2006)
  8. Chen L-Y,, "Effects of insectresistance transgenes on fecundity in rice(Oryza sativa, Poaceae) : a test for underlying costs", Am J Bot, 93, pp.94~101 (2006)
  9. Cipollini D, "Constitutive expression of methyl jasmonate-inducible responses delays reproduction and constrains fitness responses to nutrients in Arabidopsis thaliana", Evol Ecol, 24, pp.59~68 (2010)
  10. Dale PJ, "Field performance of trans genic potato plants compared with controls regenerated from tuber discs and shoot cuttings", Theor Appl Genet, 84, pp.585~591 (1992)
  11. Damgaard C, "Competitive interactions and the effect of herbivory on Bt-Brassica napus, Brassica rapa and Lolium perenne", J Appl Ecol, 46, pp.1073~1079 (2009)
  12. in the field, "Pest Manag Sci", 2009, 753~760
  13. Ellstrand NC, "Current knowledge of gene flow in plants : implications for transgene flow", Philos T Roy Soc B, 358, pp.1163~1170 (2003)
  14. Ellstrand NC, "Gene flow and introgression from domesticated plants into their wild relatives", Annu Rev Ecol Evol Syst, 30, pp.539~563 (1999)
  15. Fowler N, "Competition and coexistence in a North Carolina grassland. III. Mixtures of component species", J Ecol, 70, pp.77~92 (1982)
  16. Fredshavn JR, "Growth behavior and competitive ability of transgenic crops", Field Crop Res, 45, pp.11~18 (1996)
  17. Fredshavn JR, "Competitiveness of transgenic oilseed rape", Transgenic Res, 4, pp.142~148 (1995)
  18. Gassmann AJ, "Consequence of herbivory for the fitness cost of herbicide resistance : photosynthetic variation in the context of plant–herbivore interactions", J Evol Biol, 18, pp.447~454 (2005)
  19. Gurr SJ, "Engineering plants with increased disease resistance: how are we going to express it?", Trends Biotechnol, 23, pp.283~290 (2005)
  20. Hails RS, "Genetically modified plants-the debate continues", Trends Ecol Evol, 15, pp.14~18 (2000)
  21. Hails RS, "Genes invading new populations : a risk assessment perspective", Trends Ecol Evol, 20, pp.245~252 (2005)
  22. Hails RS, "Burial and seed survival in Brassica napus subsp. oleifera and Sinapis arvensis including a comparison of transgenic and non-transgenic lines of the crop", P Roy Soc Lond B-Bio, 264, pp.1~7 (1997)
  23. Halfhill MD, "Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes", Mol Ecol, 14, pp.3177~3189 (2005)
  24. Hilder VA, "Phenotypic cost to plants of an extra gene", Transgenic Res, 1, pp.54~60 (1991)
  25. Hsieh T-H, "Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress", Plant Physiol, 130, pp.618~626 (2002)
  26. Hsieh T-H, "Heterology expression of the Arabidopsis C-repeat", dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato, 2002, pp.129, 1086~1094
  27. Inouye BD, "Response surface experimental designs for investigating interspecific competition", Ecology, 82, pp.2696~2706 (2001)
  28. Jackson MW, "Costs and benefits of cold tolerance in transgenic Arabidopsis thaliana", Mol Ecol, 13, pp.3609~3615 (2004)
  29. Global status of commercialized biotech, "GM crops", James C, 2010, ISAAA Briefs No. 42
  30. Jung HI, "Resistance levels and fitness of protoporphyrinogen oxidase(PROTOX)inhibitor-resistant transgenic rice in paddy fields", Field Crop Res, 115, pp.125~131 (2010)
  31. Kasuga M, "Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor", Nature Biotechnol, 17, pp.287~291 (1999)
  32. Kim S, "Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer", Euphytica, 164, pp.829~839 (2008)
  33. Lavigne C, "The cost of herbicide resistance in white-chicory : ecological implications for its commercial release", Theor Appl Genet, 91, pp.1301~1308 (1995)
  34. McHughen A, "Herbicide resistant transgenic flax field test : agronomic performance in normal and sulfonylurea-containing soils", Euphytica, 55, pp.49~56 (1991)
  35. Ortelli S, "Leaf rust resistance gene Lr9 and winter wheat yield reduction : I. Yield and yield components", Crop Sci, 36, pp.1590~1595 (1996)
  36. Owen MD, "Herbicide-resistant crops and weed resistance to herbicides", Pest Manag Sci, 61, pp.301~311 (2005)
  37. Pasonen H-L, "Field performance of chitinase transgenic silver birch(Betula pendula Roth) : growth and adaptive traits", Trees Struct Funct, 22, pp.413~421 (2008)
  38. Plowman AB, "The effect of light and temperature on competition between atrazine susceptible and resistant Brassica rapa", Ann Bot, 80, pp.583~590 (1997)
  39. Purrington CB, "Fitness consequences of genetically engineered herbicide and antibiotic resistance in Arabidopsis thaliana", Genetics, 145, pp.807~814 (1997)
  40. Ramachandran S, "Intraspecific competition of an insect-resistant transgenic canola in seed mixtures", Agron J, 92, pp.368~374 (2000)
  41. Regal PJ, "The adaptive potential of genetically engineered organisms in nature", Trends Ecol Evol, 3, pp.36~38 (1988)
  42. Romeis J, "Performance of transgenic spring wheat plants and effects on non-target organisms under glasshouse and semi-field conditions", J Appl Entomol, 131, pp.593~602 (2007)
  43. Sasu MA, "Indirect costs of a nontarget pathogen mitigate the direct benefits of a virus-resistant transgene in wild Cucurbita", P Natl Acad Sci USA, 106, pp.19067~19071 (2009)
  44. Snow AA, "Costs of transgenic herbicide resistance introgressed from Brassica napus into weedy B. rapa", Mol Ecol, 8, pp.605~615 (1999)
  45. Snow AA, "A Bt transgene reduces herbivory and enhances fecundity in wild sunflowers", Ecol Appl, 13, pp.279~286 (2003)
  46. Vacher C, "Impact of ecological factors on the initial invasion of Bt transgenes into wild populations of birdseed rape(Brassica rapa)", Theor Appl Genet, 109, pp.806~814 (2004)
  47. Warwick SI, "Gene flow between GM crops and related species in Canada. In: The First Decade of Herbicide Resistant Crops in Canada, In Topics in Canadian Weed Science", pp.101~113 (2007), Canadian Weed Science Society
  48. Warwick SI, "Relative fitness of herbicideresistant and-susceptible biotypes of weeds", Phytoprotection, 75, pp.37~49 (1994)
  49. Warwick SI, "Do escaped transgenes persist in nature? The case of an herbicide resistance transgene in a weedy Brassica rapa population", Mol Ecol, 17, pp.1387~1395 (2008)
  50. Warwick SI, "Gene flow, invasiveness, and ecological impact of genetically modified crops", Ann NY Acad Sci, 1168, pp.72~99 (2009)
  51. Xia H, "Yield benefit and underlying cost of insect-resistance transgenic rice : implication in breeding and deploying transgenic crops", Field Crop Res, 118, pp.215~220 (2010)
  52. Yang K, "Modifying Myxococcus xanthus protoporphyrinogen oxidase to plant codon usage and high level of oxyfluorfen resistance in transgenic rice", Pestic Biochem Phys, 86, pp.186~194 (2006)
  53. Yoshida S, "Laboratory Manual for Physiological Studies of Rice, 3rd ed" (1976), The International Rice Research Institute

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