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Zerumbone 처리 헬리코박터 파이로리균의 전사체 분석 비교

Comparative Transcriptome Analysis of Zerumbone-Treated Helicobacter pylori

김사현|우현준|Yang Ji Yeong
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2022.06|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제50권 제2호|pp. 301-309|DOI 10.48022/mbl.2202.02009

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키워드

Helicobacter pylorinext-generation sequencingRNA-sequencingzerumbone
주제분류생물학

초록

본 연구에서는 제럼본에 처리에 의해 유도된 H. pylori 유전자의 전사적 변화를 분석하였다. NGS를 사용하여 RNA 발현 변화를 분석한 다음 그 결과를 검증하기 위해 RT-PCR 을 수행하였다. NGS 분석 결과, 1,632개의 유전자 중 총23개가 제럼본 처리에 의해 유의하게 발현이 변화된 특이발현 유전자로 분석되었다. DNA 복제와 전사, 병원성 인자 및T4SS 성분과 관련된 유전자 중 10개는 현저하게 하향 조절되었고 5개는 상향 조절되었다. RT-PCR을 이용하여 유전자 의 발현 수준을 재확인하였고 그 결과, 14개 유전자에서 NGS 와 동일하게 발현 양상이 변화하였다. RT-PCR은 제럼본 처리에 의해 10개의 유전자(dnaE, dnaQ, rpoA, rpoD, secA, flgE, flhA, virB5, virB8, virB9)의 발현 감소와 4개의 유전자(flaA, flaB, virB4, virD4)의 발현 증가를 보였다. 이러한본 연구의 결과는 제럼본이 다양한 H. pylori의 병원성과 관련된 인자들을 조절함으로써 H. pylori 감염의 잠재적인 치료제가 될 수 있음을 시사한다.

Abstract

Helicobacter pylori (H. pylori) establishes infection in the human gastric mucosa for a long time and causes severe gastric diseases such as peptic ulcer and gastric cancer. When H. pylori is exposed to the antibacterial agents or inhibitors, the expression of pathogenic associated genes could be altered. In this study, we analyzed the transcriptional changes of H. pylori genes induced by zerumbone treatment. RNA expression changes were analyzed using next-generation sequencing (NGS), and then reverse transcription-polymerase chain reaction (RT-PCR) was performed to verify the results. As a result of NGS analysis, a total of 23 out of 1,632 genes were differentially expressed by zerumbone treatment. RT-PCR confirmed that zerumbone treatment regulated the expression level of 14 genes. Among the genes associated with DNA replication, transcription, virulence factors and T4SS components, 10 genes (dnaE, dnaQ, rpoA, rpoD, secA, flgE, flhA, virB5, virB8 and virB9) were significantly down-regulated and 4 genes (flaA, flaB, virB4 and virD4) were up-regulated. The results of our current study imply that zerumbone might be a potential therapeutic agent for H. pylori infection by regulating factors related to various H. pylori pathogenicity.

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Comparative Transcriptome Analysis of Caryophyllene- Treated Helicobacter pylori김사현, 김종배, 양지영, 우현준, Kim Hyun Woo, Kwon Hye Jin한국미생물·생명공학회 · 한국미생물·생명공학회지2021 · 제49권 제3호Zerumbone 처리에 따른 Helicobacter pylori의 단백질 비교분석김사현대한임상검사과학회 · 대한임상검사과학회지2018 · 제50권 제3호Plumbagin Inhibits Expression of Virulence Factors and Growth of Helicobacter pylori문철, 박민, 김사현, 김종배, 엄용빈, 우현준, 이민호한국미생물·생명공학회 · 한국미생물·생명공학회지2016 · 제44권 제2호오수유 메탄올 추출물이 Helicobacter pylori 병원성 관련유전자 발현에 미치는 영향김종배, 양지영, 이평재대한임상검사과학회 · 대한임상검사과학회지2019 · 제51권 제3호Expression of MicroRNA in Host Cells Infected with Helicobacter pylori김진호, 박장호, 이선미, 이진혁, 정석후, 정준원, 정진용거트앤리버 소화기연관학회협의회 · Gut and Liver2017 · 제11권 제3호Helicobacter pylori의 한약 치료에 대한 연구박상은, 이승연, 홍상훈대한한방내과학회 · 대한한방내과학회지2012 · 제33권 제1호

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