한국형 프로바이오틱스에 의한 쓴메밀 내 rutin의 생물전환
Bioconversion of Rutin in Tartary Buckwheat by the Korean Indigenous Probiotics
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초록
In this study, bioconversion of rutin to quercetin was confirmed by the fermentation of Korean indigenous probiotics and tartary buckwheat. Based on whole genome sequencing of 17 probiotics species, α-rhamnosidase, related to bioconversion of isoquercetin (quercetin 3-β-D glucoside) from rutin, is identified in the genome of CBT BG7, LC5, LR5, LP3, LA1, and LGA1. β-Glucosidase, related to bioconversion of isoquercetin to quercetin, is identified in the genome of all 17 species. Among the 17 probiotics species, 6 probiotics including CBT BG7, LR5, LP3, LA1, LGA1 and ST3 performed the bioconversion of rutin to quercetin up to 21.5 ± 0.3% at 7 days after fermentation. The fermentation of each probiotics together with enzyme complex Cellulase KN® was conducted to reduce the time of bioconversion. As a result, CBT LA1 which showed the highest yield of bioconversion of 21.5 ± 0.3% when the enzyme complex was not added showed high bioconversion yield of 84.6 ± 0.5% with adding the enzyme complex at 1 day after fermentation. In particular, CBT ST3 (96.2 ± 0.4%), SL6 (90.1 ± 1.4%) and LP3 (90.0 ± 0.4%) showed high yield of bioconversion more than 90%. In addition, such probiotics including high levels in quercetin indicated the inhibitory effects of NO production in LPS-induced RAW264.7 cells. In this study, we confirmed that the fermentation of Korean indigenous probiotics and enzyme complex together with roasted tartary buckwheat increased the content of quercetin and reduced the time of bioconversion of rutin to quercetin which is a bioactive compound related to anti-inflammatory, antioxidants, anti-obesity, and anti-diabetes.
Abstract
In this study, bioconversion of rutin to quercetin was confirmed by the fermentation of Korean indigenous probiotics and tartary buckwheat. Based on whole genome sequencing of 17 probiotics species, α-rhamnosidase, related to bioconversion of isoquercetin (quercetin 3-β-D glucoside) from rutin, is identified in the genome of CBT BG7, LC5, LR5, LP3, LA1, and LGA1. β-Glucosidase, related to bioconversion of isoquercetin to quercetin, is identified in the genome of all 17 species. Among the 17 probiotics species, 6 probiotics including CBT BG7, LR5, LP3, LA1, LGA1 and ST3 performed the bioconversion of rutin to quercetin up to 21.5 ± 0.3% at 7 days after fermentation. The fermentation of each probiotics together with enzyme complex Cellulase KN® was conducted to reduce the time of bioconversion. As a result, CBT LA1 which showed the highest yield of bioconversion of 21.5 ± 0.3% when the enzyme complex was not added showed high bioconversion yield of 84.6 ± 0.5% with adding the enzyme complex at 1 day after fermentation. In particular, CBT ST3 (96.2 ± 0.4%), SL6 (90.1 ± 1.4%) and LP3 (90.0 ± 0.4%) showed high yield of bioconversion more than 90%. In addition, such probiotics including high levels in quercetin indicated the inhibitory effects of NO production in LPS-induced RAW264.7 cells. In this study, we confirmed that the fermentation of Korean indigenous probiotics and enzyme complex together with roasted tartary buckwheat increased the content of quercetin and reduced the time of bioconversion of rutin to quercetin which is a bioactive compound related to anti-inflammatory, antioxidants, anti-obesity, and anti-diabetes.
참고문헌 (32)
- 김수정, "메밀의 국내외 생산동향과 전망", 한국국제농업개발학회지, 29(3), pp.226~233 (2017), 한국국제농업개발학회[DOI]
- 김수정, "쓴메밀 유전자원의 종자특성과 유용성분 변이에 관한 자원 정보 구축", 한국자원식물학회지, 33(5), pp.446~459 (2020), 한국자원식물학회
- Agricultural guide Buckwheat, "Kim SJ", Rural Development Administration, 07~90 (2018)
- Development of new cultivar and technology of cultivation and utilization in tartary buckwheat as a new agricultural material, "Park CH", Ministry of Agriculture, 12~197 (2006)
- Ikeda K, "Buckwheat composition, chemistry, and processing", Adv. Food Nutr. Res, 44, pp.395~434 (2002)
- 강현우, "메밀 추출물의 항산화 및 항염증 효능", Culinary Science & Hospitality Research, 20(6), pp.190~199 (2014), (사)한국조리학회[DOI]
- 함승시, "메밀싹 추출물의 항산화 효과 및 유전독성억제 효과", 한국식품영양과학회지, 36(8), pp.955~959 (2007), 한국식품영양과학회
- Lee CC, "Fagopyrum tataricum (buckwheat) improved high-glucose-induced insulin resistance in mouse hepatocytes and diabetes in fructose-rich diet induced mice", Exp. Diabetes Res, 2012, pp.375673~ (2012)
- Kim DW, "Germinated buckwheat extract decreases blood pressure and nitrotyrosine immunoreactivity in aortic endothelial cells in spontaneously hypertensive rats", Phytother. Res, 23, pp.993~998 (2009)
- 장광진, "타타리메밀싹 발효추출물의 영양성분 및 생리활성", 한국자원식물학회지, 23(2), pp.131~137 (2010), 한국자원식물학회
- 윤보라, "쓴메밀 및 단메밀 에탄올 추출물의 항산화 및 지방세포 분화억제 효과", Food Science and Preservation, 19(1), pp.123~130 (2012), 한국식품저장유통학회
- Nam HK, "Quercetin production from rutin by a thermostable β-rutinosidase from Pyrococcus furiosus", Biotechnol. Lett, 34, pp.483~489 (2012)
- Kim JA, "Study on the anti-obesity effects of germinatedbuckwheat", Ms, pp.2006
- Fukushima T, "Anti-inflammatory effect of buckwheat sprouts in lipopolysaccharide-activated human colon cancer cells and mice", Biosci. Biotechol. Biochem, 72, pp.3148~3157 (2008)
- Holasova M, "Buckwheat-the source of antioxidant activity in functional foods", Food Res. Int, 35, pp.207~211 (2002)
- 김수정, "일반메밀과 쓴메밀 종실 추출물의 RAW 264.7 대식세포에서 LPS에 의해 유도되는 iNOS 및 염증성 사이토카인 발현 저해를 통한 항염증 효과 비교", 한국식품과학회지, 51(6), pp.565~575 (2019), 한국식품과학회[DOI]
- Erlund I, "Review of the flavonoids quercetin, hesperetin, and naringenin. Dietary sources, bioactivities, bioavailability and epidemiology", Nutr. Res, 24, pp.851~874 (2004)
- Zhang ZL, "Bioactive compounds in functional buckwheat food", Food Res. Int, 49, pp.389~395 (2012)
- 김지현, "Kaempferol, quercetin 및 그 배당체들의 apoptosis 조절을 통한 신경세포 보호 효과", 한국산학기술학회논문지, 20(2), pp.286~293 (2019), 한국산학기술학회[DOI]
- Heim KE, "Flavonoid antioxidants:Chemistry, metabolism and structureactivity relationships", J. Nutr. Biochem, 13, pp.572~584 (2002)
- Ademosun AO, "Antioxidative properties and effect of quercetin and its glycosylated form (Rutin) on acetylcholinesterase and butyrylcholinesterase activities", J. Evid. Based Complementary Altern. Med, 21, pp.NP11~NP17 (2015)
- 양진우, "Conversion of Rutin to Quercetin by Acid Treatment in Relation to Biological Activities", Preventive Nutrition and Food Science, 24(3), pp.313~320 (2019), 한국식품영양과학회[DOI]
- Kim GN, "Protective mechanism of quercetin and rutin on 2,2’-azobis(2-amidinopropane) dihydrochloride or Cu2+-induced oxidative stress in HepG2 cells", Toxicol. in Vitro, 25, pp.138~144 (2011)
- Salehi B, "Therapeutic potential of quercetin : New insights and perspectives for human health", ACS Omega, 5, pp.11849~11872 (2020)
- Zuo Y, "Highly efficient bioconversion of flavonoid glycosides from citrus processing wastes in solvent-buffer systems", Green Chem, 22, pp.3196~3207 (2020)
- Dewi RT, "Bioconversion of quercetin glucosides from Dendrophthoe pentandra leaf using Aspergillus acueletus LS04-3", AIP Conference Proceedings, 2175, pp.020048~ (2019)
- Huynh NT, "Bioconversion of kaempferol and quercetin glucosides from plant sources using Rhizopus spp", Fermentation, 4, pp.1~9 (2018)
- Amaretti A, "Hydrolysis of the rutinose-conjugates flavonoids rutin and hesperidin by the gut microbiota and bifidobacteria", Nutrients, 7, pp.2788~2800 (2015)
- 최지원, "비피도박테리움 CBT BG7, BR3, BL3의 진세노사이드 전환능", 한국미생물·생명공학회지, 50(3), pp.395~403 (2022), 한국미생물·생명공학회[DOI]
- 김수정, "가공 및 추출 방법에 따른 일반메밀차와 쓴메밀차의 루틴 함량 및 항산화 활성 비교", 한국식품과학회지, 54(2), pp.185~195 (2022), 한국식품과학회
- Park CM, "Biotransformation of flavonoids by newly isolated and characterized Lactobacillus pentosus NGI01 strain from Kimchi", Microorganisms, 9, pp.1075~ (2021)
- Mueller M, "Rhamnosidase activity of selected probiotics and their ability to hydrolyse flavonoid rhamnoglucosides", Bioprocess Biosyst. Eng, 41, pp.221~228 (2018)