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PET 생분해에 관여하는 방선균 유래 PETase 유전자의 이종숙주 발현

Heterologous Expression of Streptomyces PETase Gene Involved in PET Biodegradation

김응수|Choi Si-Sun|Yang Soo-been|Yoo Yeon-Jin
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2022.12|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제50권 제4호|pp. 501-507|DOI 10.48022/mbl.2211.11002

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키워드

StreptomycesPETasePET degradationheterologous expression
주제분류생물학

초록

대표적인 플라스틱 소재인 PET (Polyethylene terephthalate) 는 높은 내구성, 경제성과 같은 유용한 물리화학적 특성으로병, 섬유, 용기 등 다양한 산업 분야에 사용되고 있다. 최근일회용품을 비롯한 플라스틱 사용량 증가로 인해, 이를 처리하기 위한 방법이 필요한 상황이다. 기존의 매립, 소각 등과같이 자연상태에 노출되는 방법과 달리 최근 미생물을 이용한 친환경적인 방법이 주목받고 있다. 본 연구에서는 PETase 유전자를 가지고 있는 토양 유래 방선균 Streptomyces. javensis Inha503를 선별하고, agar plate diffusion assay 를 통해 PU (Polyurethane) 가수분해 능력을 확인하였다. 해당 균주를 PET과 함께 한달 간 배양하였고, 주사전자현미경을 통해 PET 분해능력을 확인하였다. 또한, S. javensis Inha503 유전체 탐색에서 선별된 PETase 유전자를 PET 분해능이 없는 이종숙주 S. lividans와 S. coelicolor 균주에 도입하여 PET 분해능을 확인함으로써, 방선균 유래 PETase 유전자의 활성을 최초로 확인하였다.

Abstract

PET (Polyethylene terephthalate), a representative plastic material, has useful physicochemical properties such as high durability and economic feasibility, and is used in various industrial fields such as bottles, fibers, and containers. Due to the recent increase in plastic usage including disposable products, ecofriendly strategy using microorganisms have drawn attention differentiated from conventional landfill and incineration methods. In this study, a soil-derived Streptomyces javensis Inha503 containing a PETase gene was selected and the ability to hydrolyze PU (Polyurethane) was confirmed through agar plate diffusion assay. This strain was cultured with PET for a month, and PET decomposition ability was also confirmed through a scanning electron microscope. Moreover, cloning and heterologous expression of S. javensis Inha503 PETase gene exhibited PET activity in the PETase non-containing S. coelicolor, confirming for the first time the presence of functional PETase gene in Streptomyces species.

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