Analysis of Gut Microbiota Composition in Obese and Normal Weight Algerian Women by 16S rRNA Gene Amplicon Sequencing
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The prevalence of obesity is increasing throughout the world, imposing a heavy burden on individuals, society and the economy. The gut microbiota (GM) is recognized as an important contributing factor on the development of obesity. Several studies around the world have reported differences in GM composition between obese and lean individuals. However, the GM composition of either obese (OB) or normal weight (NW) Algerian individuals are lacking. Therefore, we conducted the first study in Algeria to characterize and compare the GM composition in OB and NW adult women. Fecal microbiota of 10 Algerian women, 5 OB, with an average Body Mass Index (BMI) of 41.22 ± 5.05, and 5 NW (average BMI of 20.69 ± 0.75), from the region of Tlemcen in Algeria, were analyzed by 16S rRNA gene amplicon sequencing on NovaSeq 6000 Illumina platform. Alpha and Beta diversity analysis did not reveal significant differences between the two groups. However, the GM composition of our cohort was significantly different from that of Europeans, Americans and Asians, showing predominance of the phyla Firmicutes and Actinobacteria, whereas the phylum Bacteroidota was underrepresented. Obese individuals had a significant increase in the genera Enterococcus, Blautia, Romboutsia, Catenisphaera and Clostridium sensu stricto 1, and a reduced abundance of the genera Bifidobacterium, Ligilactobacillus, Catenibacterium, Collinsella and Akkermansia. This pilot study revealed a distinctive GM composition in Algerian women, highlighting the need for further larger cohort studies to establish the composition of a typical GM composition in the Algerian population.
Abstract
The prevalence of obesity is increasing throughout the world, imposing a heavy burden on individuals, society and the economy. The gut microbiota (GM) is recognized as an important contributing factor on the development of obesity. Several studies around the world have reported differences in GM composition between obese and lean individuals. However, the GM composition of either obese (OB) or normal weight (NW) Algerian individuals are lacking. Therefore, we conducted the first study in Algeria to characterize and compare the GM composition in OB and NW adult women. Fecal microbiota of 10 Algerian women, 5 OB, with an average Body Mass Index (BMI) of 41.22 ± 5.05, and 5 NW (average BMI of 20.69 ± 0.75), from the region of Tlemcen in Algeria, were analyzed by 16S rRNA gene amplicon sequencing on NovaSeq 6000 Illumina platform. Alpha and Beta diversity analysis did not reveal significant differences between the two groups. However, the GM composition of our cohort was significantly different from that of Europeans, Americans and Asians, showing predominance of the phyla Firmicutes and Actinobacteria, whereas the phylum Bacteroidota was underrepresented. Obese individuals had a significant increase in the genera Enterococcus, Blautia, Romboutsia, Catenisphaera and Clostridium sensu stricto 1, and a reduced abundance of the genera Bifidobacterium, Ligilactobacillus, Catenibacterium, Collinsella and Akkermansia. This pilot study revealed a distinctive GM composition in Algerian women, highlighting the need for further larger cohort studies to establish the composition of a typical GM composition in the Algerian population.
참고문헌 (85)
- "Obesity and Overweight" (2021)[URL]
- Ortega MA, "Type 2 diabetes mellitus associated with obesity (Diabesity). The central role of gut microbiota and its translational applications", Nutrients, 12, pp.2749~ (2020)
- Hruby A, "The epidemiology of obesity : A big picture", Pharmacoeconomics, 33, pp.673~689 (2015)
- Ley RE, "Microbial ecology : Human gut microbes associated with obesity", Nature, 444, pp.1022~1023 (2006)
- Turnbaugh PJ, "A core gut microbiome in obese and lean twins", Nature, 457, pp.480~484 (2009)
- Jandhyala SM, "Role of the normal gut microbiota", World J. Gastroenterol, 21, pp.8787~8803 (2015)
- Lee CJ, "Gut microbiome and its role in obesity and insulin resistance", Ann. N. Y. Acad. Sci, 1461, pp.37~52 (2020)
- Syromyatnikov M, "Characteristics of the gut bacterial composition in people of different nationalities and religions", Microorganisms, 10, pp.1866~ (2022)
- Gupta VK, "Geography, ethnicity or subsistence-specific variations in human microbiome composition and diversity", Front. Microbiol, 8, pp.1162~ (2017)
- Le Chatelier E, "Richness of human gut microbiome correlates with metabolic markers", Nature, 500, pp.541~546 (2013)
- Yasir M, "Comparison of the gut microbiota of people in France and Saudi Arabia", Nutr. Diabetes, 5, pp.e153~ (2015)
- Andoh A, "Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population", J. Clin. Biochem. Nutr, 59, pp.65~70 (2016)
- Palmas V, "Gut microbiota markers associated with obesity and overweight in Italian adults", Sci. Rep, 11, pp.5532~ (2021)
- Pesoa SA, "Comparison of Argentinean microbiota with other geographical populations reveals different taxonomic and functional signatures associated with obesity", Sci. Rep, 11, pp.7762~ (2021)
- Turnbaugh PJ, "An obesity-associated gut microbiome with increased capacity for energy harvest", Nature, 444, pp.1027~1031 (2006)
- Turnbaugh PJ, "The core gut microbiome, energy balance and obesity", J. Physiol, 587, pp.4153~4158 (2009)
- Dominianni C, "Sex, body mass index, and dietary fiber intake influence the human gut microbiome", PLoS One, 10, pp.e0124599~ (2015)
- "Algeria, the burden of malnutrition at a glance" (2021)[URL]
- William S, "Bacterial DNA isolation CTAB protocol", Sigma, 50, pp.6876~ (2012)
- Klindworth A, "Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies", Nucleic Acids Res, 41, pp.e1~ (2013)
- Caporaso JG, "Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample", Proc. Natl. Acad. Sci. USA, 108, pp.4516~4522 (2011)
- Magoč T, "FLASH : Fast length adjustment of short reads to improve genome assemblies", Bioinformatics, 27, pp.2957~2963 (2011)
- Bokulich NA, "Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing", Nat. Methods, 10, pp.57~59 (2012)
- Quast C, "The SILVA ribosomal RNA gene database project : improved data processing and web-based tools", Nucleic Acids Res, 41(D596), pp.D590~ (2013)
- Edgar RC, "UCHIME improves sensitivity and speed of chimera detection", Bioinformatics, 27, pp.2194~2200 (2011)
- Callahan BJ, "DADA2 : High-resolution sample inference from Illumina amplicon data", Nat. Methods, 13, pp.581~583 (2016)
- Bolyen E, "Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2", Nat. Biotechnol, 37, pp.852~857 (2019)
- Wall L, "Programming perl" (2000), O’Reilly Media
- "R: A Language and Environment for Statistical Computing" (2021)[URL]
- Douglas GM, "PICRUSt2 for prediction of metagenome functions", Nat. Biotechnol, 38, pp.685~688 (2020)
- Segata N, "Metagenomic biomarker discovery and explanation", Genome Biol, 12, pp.R60~ (2011)
- Gruneck L, "Gut microbiota of obese and diabetic Thai subjects and interplay with dietary habits and blood profiles", PeerJ, 8, pp.e9622~ (2020)
- Duan M, "Characteristics of gut microbiota in people with obesity", PLoS One, 16, pp.e0255446~ (2021)
- Lin Y, "Combing fecal microbial community data to identify consistent obesity-specific microbial signatures and shared metabolic pathways", iScience, 26, pp.106476~ (2023)
- Davis SC, "Gut microbiome diversity influenced more by the Westernized dietary regime than the body mass index as assessed using effect size statistic", Microbiologyopen, 6, pp.e00476~ (2017)
- Gao X, "Body mass index differences in the gut microbiota are gender specific", Front. Microbiol, 9, pp.1250~ (2018)
- Aljazairy EA, "Influence of adiposity on the gut microbiota composition of arab women : A case-control study", Biology, 11, pp.1586~ (2022)
- Yin XQ, "The association between fecal short-chain fatty acids, gut microbiota, and visceral fat in monozygotic twin pairs", Diabetes Metab. Syndr. Obes, 15, pp.359~368 (2022)
- Rinninella E, "What is the healthy gut microbiota composition? A changing ecosystem across age, environment, diet, and diseases", Microorganisms, 7, pp.14~ (2019)
- Borgo F, "Body mass index and sex affect diverse microbial niches within the gut", Front. Microbiol, 9, pp.213~ (2018)
- Crovesy L, "Profile of the gut microbiota of adults with obesity : a systematic review", Eur. J. Clin. Nutr, 74, pp.1251~1262 (2020)
- Pinart M, "Gut microbiome composition in obese and nonobese persons : A systematic review and meta-analysis", Nutrients, 14, pp.12~ (2022)
- Qin J, "A human gut microbial gene catalog established by metagenomic sequencing", Nature, 464, pp.59~65 (2010)
- Yang B, "Diversity of gut microbiota and bifidobacterial community of Chinese subjects of different ages and from different regions", Microorganisms, 8, pp.1108~ (2020)
- Angelakis E, "Gut microbiome and dietary patterns in different Saudi populations and monkeys", Sci. Rep, 6, pp.32191~ (2016)
- Angelakis E, "Comparison of the gut microbiota of obese individuals from different geographic origins", New Microbes New Infect, 27, pp.40~47 (2019)
- Jain A, "Similarities and differences in gut microbiome composition correlate with dietary patterns of Indian and Chinese adults", AMB Express, 8, pp.104~ (2018)
- Tyakht AV, "Human gut microbiota community structures in urban and rural populations in Russia", Nat. Commun, 4, pp.2469~ (2013)
- Gomes AC, "The human gut microbiota : Metabolism and perspective in obesity", Gut Microbes, 9, pp.308~325 (2018)
- Magne F, "The firmicutes", bacteroidetes ratio: A relevant marker of gut dysbiosis in obese patients?, 2020, pp.12, 1474~
- Collado MC, "Distinct composition of gut microbiota during pregnancy in overweight and normal-weight women", Am. J. Clin. Nutr, 88, pp.894~899 (2008)
- Schwiertz A, "Microbiota and SCFA in lean and overweight healthy subjects", Obesity, 18, pp.190~195 (2010)
- Ismail NA, "Frequency of Firmicutes and Bacteroidetes in gut microbiota in obese and normal weight Egyptian children and adults", Arch. Med. Sci, 7, pp.501~507 (2011)
- Duncan SH, "Human colonic microbiota associated with diet, obesity and weight loss", Int. J. Obes, 32, pp.1720~1724 (2008)
- Mai V, "Associations between dietary habits and body mass index with gut microbiota composition and fecal water genotoxicity : An observational study in African American and Caucasian American volunteers", Nutr. J, 8, pp.49~ (2009)
- Zupancic ML, "Analysis of the gut microbiota in the old order Amish and its relation to the metabolic syndrome", PLoS One, 7, pp.e43052~ (2012)
- Merra G, "Influence of mediterranean diet on human gut microbiota", Nutrients, 13, pp.7~ (2021)
- Garcia-Mantrana I, "Shifts on gut microbiota associated to mediterranean diet adherence and specific dietary intakes on general adult population", Front. Microbiol, 9, pp.890~ (2018)
- Tomova A, "The effects of vegetarian and vegan diets on gut microbiota", Front. Nutr, 6, pp.47~ (2019)
- Binda C, "Actinobacteria : A relevant minority for the maintenance of gut homeostasis", Dig. Liver Dis, 50, pp.421~428 (2018)
- Rivière A, "Bifidobacteria and butyrate-producing colon bacteria : Importance and strategies for their stimulation in the human gut", Front. Microbiol, 7, pp.979~ (2016)
- Venegas DP, "Short chain fatty acids(SCFAs)mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases", Front. Immunol, 10, pp.277~ (2019)
- Koliada A, "Association between body mass index and Firmicutes", Bacteroidetes ratio in an adult Ukrainian population, 2017, pp.17, 120~
- Chávez-Carbajal A, "Gut microbiota and predicted metabolic pathways in a sample of Mexican women affected by obesity and obesity plus metabolic syndrome", Int. J. Mol. Sci, 20, pp.438~ (2019)
- Sarmiento MRA, "Obesity, xenobiotic intake and antimicrobial-resistance genes in the human gastrointestinal tract : A comparative study of eutrophic, overweight and obese individuals", Genes, 10, pp.349~ (2019)
- Mahjoub Khachroub A, "First characterization of the intestinal microbiota in healthy Tunisian adults using 16S rRNA gene sequencing", FEMS Microbiol. Lett, 370, pp.fnad059~ (2023)
- Zeng Q, "Discrepant gut microbiota markers for the classification of obesity-related metabolic abnormalities", Sci. Rep, 9, pp.13424~ (2019)
- Therdtatha P, "Gut microbiome of Indonesian adults associated with obesity and type 2 diabetes : A cross-sectional study in an Asian city, Yogyakarta", Microorganisms, 9, pp.897~ (2021)
- Gong J, "Gut microbiota characteristics of people with obesity by metaanalysis of existing datasets", Nutrients, 14, pp.2993~ (2022)
- Chang CS, "An overview of microbiome based strategies on anti-obesity", Kaohsiung J. Med. Sci, 35, pp.7~16 (2019)
- Walker AW, "Dominant and diet-responsive groups of bacteria within the human colonic microbiota", ISME J, 5, pp.220~230 (2011)
- Gomez-Arango LF, "Low dietary fiber intake increases Collinsella abundance in the gut microbiota of overweight and obese pregnant women", Gut Microbes, 9, pp.189~201 (2018)
- Karlsson CLJ, "The microbiota of the gut in preschool children with normal and excessive body weight", Obesity, 20, pp.2257~2261 (2012)
- Everard A, "Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity", Proc. Natl. Acad. Sci. USA, 110, pp.9066~9071 (2013)
- Zhou Q, "Gut bacteria Akkermansia is associated with reduced risk of obesity : evidence from the American Gut Project", Nutr. Metab, 17, pp.1~9 (2020)
- Aggarwal V, "Disease-associated dysbiosis and potential therapeutic role of Akkermansia muciniphila, a mucus degrading bacterium of gut microbiome", Folia Microbiologica, 67, pp.811~824 (2022)
- Shankar V, "Differences in gut metabolites and microbial composition and functions between Egyptian and U.S. children are consistent with their diets", mSystems, 2, pp.10~1128 (2017)
- Zheng, J, "A taxonomic note on the genus Lactobacillus : Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae", Int. J. Syst. Evol. Microbiol, 70, pp.2782~2858 (2020)
- Armougom F, "Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and Methanogens in anorexic patients", PLoS One, 4, pp.1~8 (2009)
- Million M, "Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii", Int. J. Obes, 36, pp.817~825 (2012)
- Fernández-Navarro T, "Different intestinal microbial profile in Overweight and obese subjects consuming a diet with low content of fiber and antioxidants", Nutrients, 9, pp.551~ (2017)
- Chen X, "Alteration of the gut microbiota associated with childhood obesity by 16S rRNA gene sequencing", PeerJ, 8, pp.e8317~ (2020)
- Ahmad MA, "Association of the gut microbiota with clinical variables in obese and lean Emirati subjects", Front. Microbiol, 14, pp.1182460~ (2023)
- Hill EB, "The microbiome, epigenome, and diet in adults with obesity during behavioral weight loss", Nutrients, 15, pp.3588~ (2023)
- Yoshida N, "Bacteroides vulgatus and Bacteroides dorei reduce gut microbial Lipopolysaccharide production and inhibit atherosclerosis", Circulation, 138, pp.2486~2498 (2018)
