Selected D7 Antibody Inhibit Cancer Cell Growth
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We developed to screen for antibodies that promote cell growth in serum-free conditions using a human antibody library. The antibody library was engineered to be anchored near the cell surface and delivered to cells using a lentivirus to introduce the antibody genes. When treated cells cultured under serum-free conditions, viable cells were obtained and antibodies were selected by PCR. Although selected D7 antibody did not induce cell growth and proliferation under serum-free conditions as initially expected, we unexpectedly discovered that D7 antibody significantly inhibits MCF cancer cells in 10% FBS condition. Overall, our findings suggest that D7 antibody, discovered through this novel method, is a promising candidate for future cancer therapies.
Abstract
We developed to screen for antibodies that promote cell growth in serum-free conditions using a human antibody library. The antibody library was engineered to be anchored near the cell surface and delivered to cells using a lentivirus to introduce the antibody genes. When treated cells cultured under serum-free conditions, viable cells were obtained and antibodies were selected by PCR. Although selected D7 antibody did not induce cell growth and proliferation under serum-free conditions as initially expected, we unexpectedly discovered that D7 antibody significantly inhibits MCF cancer cells in 10% FBS condition. Overall, our findings suggest that D7 antibody, discovered through this novel method, is a promising candidate for future cancer therapies.
참고문헌 (20)
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