Ecophysiology of seed dormancy and germination in four Lonicera (Caprifoliaceae) species native to Korea
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15–25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50–80% of the length of the seeds’ development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L.insularis and L. harae germinated within 4 weeks after sowing at 15 °C and 20 °C, respectively. Contrarily, L.praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 °C, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 °C, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 °C). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 °C) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 °C, 20 °C, 15 °C, and 20 °C, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.
Abstract
Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15–25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50–80% of the length of the seeds’ development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L.insularis and L. harae germinated within 4 weeks after sowing at 15 °C and 20 °C, respectively. Contrarily, L.praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 °C, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 °C, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 °C). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 °C) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 °C, 20 °C, 15 °C, and 20 °C, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.
참고문헌 (29)
- Adams CA, "Trait stasis versus adaptation in disjunct relict species : evolutionary changes in seed dormancy-breaking and germination requirements in a subclade of Aristolochia subgenus Siphisia(Piperales)", Seed Sci Res, 15, pp.161~173 (2005)
- Baskin CC, "Seeds: ecology, biogeography, and, evolution of dormancy and germination" (1998), Academic
- Baskin CC, "When breaking seed dormancy is a problem try a movealong experiment", Nat Plants J, 4, pp.17~21 (2003)
- Baskin CC, "Germination seeds of wildflowers, an ecological perspective", HortTechnology, 14, pp.467~473 (2004)
- Baskin CC, "Two types of morphophysiological dormancy in seeds of two genera(Osmorhiza and Erythronium)with an Arcto-Tertiary distribution pattern", Am J Bot, 82, pp.293~298 (1995)
- Baskin JM, "Germination ecophysiology of the woodland herb Osmorhiza longistylis(Umbelliferae)", Am J Bot, 71, pp.687~692 (1984)
- Baskin JM, "Nondeep complex morphophysiological dormancy in seeds of Osmorhiza claytonii(Apiaceae)", Am J Bot, 78, pp.588~593 (1991)
- Baskin JM, "A classification system for seed dormancy", Seed Sci Res, 14, pp.1~16 (2004)
- Baskin JM, "Physical dormancy in seeds of Dodonaea viscosa(Sapindales, Sapindaceae)from Hawaii", Seed Sci Res, 14, pp.81~90 (2004)
- De Souza TV, "Water absorption and dormancy-breaking requirements of physically dormant seeds of Schizolobium parahyba(Fabaceae–Caesalpinioideae)", Seed Sci Res, 22, pp.169~176 (2012)
- Doussi MA, "Ecophysiology of seed germination in Mediterranean geophytes. 1. Muscari spp", Seed Sci Res, 12, pp.193~201 (2002)
- Finch-Savage WE, "Seed dormancy and the control of germination", New Phytol, 171, pp.501~523 (2006)
- Geneve RL, "Impact of temperature on seed dormancy", HortScience, 38, pp.336~340 (2003)
- Hidayati SN, "Dormancy-breaking and germination requirements for seeds of Diervilla lonicera(Caprifoliaceae), a species with underdeveloped linear embryos", Can J Bot, 78, pp.1199~1205 (2000)
- Hidayati SN, "Dormancy-breaking and germination requirements of seeds of four Lonicera species(Caprifoliaceae)with underdeveloped spatulate embryos", Seed Sci Res, 10, pp.459~469 (2000)
- 정금선, "엽록체 DNA를 이용한 섬괴불나무(Lonicera insularis Nakai)의 종내변이 및 지리학적 연구", 식물분류학회지, 44(3), pp.202~207 (2014), 한국식물분류학회
- 김선미, "괴불나무잎(Lonicera maackii)의 성분", 생약학회지, 47(2), pp.117~121 (2016), 한국생약학회
- "Korea National Arboretum" (2019)[URL]
- Lee TB, "Coloured flora of Korea" (2003), Hyangmunsa
- Martin AC, "The comparative internal morphology of seeds", Am Midl Naturalist, 36, pp.513~660 (1946)
- Naugžemys D, "RAPD based study of genetic variation and relationships among Lonicera germplasm accessions", Biologija, 53, pp.34~39 (2007)
- Nikolaeva MG, "germination", pp.51~74 (1977), North-Holland Publishing
- Phartyal SS, "Temperature requirements differ for the two stages of seed dormancy break in Aegopodium podagraria(Apiaceae), a species with deep complex morphophysiological dormancy", Am J Bot, 96, pp.1086~1095 (2009)
- Jin Ah Ryuk, "Discrimination of Lonicera japonica and Lonicera confusa using chemical analysis and genetic marker", 대한본초학회지, 27(6), pp.15~21 (2012), 대한본초학회
- Santiago A, "Species-specific environmental requirements to break seed dormancy : implications for selection of regeneration niches in three Lonicera(Caprifoliaceae)species", Bot, 91, pp.225~233 (2012)
- Theis N, "Phylogenetics of the Caprifolieae and Lonicera(Dipsacales)based on nuclear and chloroplast DNA sequences", Syst Bot, 33, pp.776~783 (2008)
- Walck JL, "Seed germination ecophysiology of the Asian species Osmorhiza aristata(Apiaceae) : comparison with its North American congeners and implications for evolution of types of dormancy", Am J Bot, 89, pp.829~835 (2002)
- Walck JL, "Seed germination and seedling development ecology in world-wide populations of a circumboreal Tertiary relict", AoB plants, 2012, pp.pls007~ (2012)
- Yuan Y, "Genetic variation and metabolic pathway intricacy govern the active compound content and quality of the Chinese medicinal plant Lonicera japonica thunb", BMC Genomics, 13, pp.195~ (2012)