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Effect of iron on the proteolytic activity of live Uronema marinum (Ciliata: Scoticociliatida) measured by fluorescence polarization

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Abstract

Effect of iron on the extracellular proteolytic activity of live Uronema marium was determined by fluorescence polarization (FP) method. Supplementation of 0.5 and 5.0 μM iron significantly increased caseinolytic activity of live U. marinum. In contrast, supplementation of 50 μM iron showed no significant differences in FP values compared to the control. The present result suggests that iron in cultured water or skin tissue of olive flounder may influence on the penetration and establishment of U. marinum, correlating with modulation of extracellular protease activity of the ciliates.

참고문헌 (14)

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관련 논문AI추천

Real Time Measurement of Protease Activity of Live Uronema marinum (Ciliata: Scuticociliatida) by Fluorescence Polarization Assay권세련, 김기홍, 이은혜, 이형호, 정준기, ChunSooKim한국수산과학회 · Fisheries and Aquatic Sciences2002 · 제5권 제4호Proteases in Cell Lysate of Uronema marinum(Ciliata: Scuticociliatida), an Opportunistic Pathogen of Cultured Olive Flounder(Paralichthys olivaceus)ChunSooKim, HyungHoLee, JaeBumCho, JoonKiChung, KiHongKim, KyoungJinAhn, SeRyunKwon한국수산과학회 · Fisheries and Aquatic Sciences2002 · 제5권 제3호Membrane Lipids of a Marine Ciliate Protozoan Uronema marinumHyungHoLee, JoonKiChung, JungSooSeo, KiHongKim한국수산과학회 · Fisheries and Aquatic Sciences2003 · 제6권 제3호Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence공현희, 김원태, 유학선, 정동일, 정해진, 하영란, 홍연철대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2006 · 제44권 제4호Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions김용석, 류재숙, 박순정, 송현욱, 최익화대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2006 · 제44권 제4호In vitro efficacy of formalin, hydrogen peroxide and copper sulfate on the scuticocilliate Uronema marinum atlow salinityBo Young Jee, Jin Woo Kim, Mi Ra Jo, Mi Seon Park수산생명의학회 · 한국어병학회지2002 · 제15권 제3호

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