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국내산 봉독의 어류병원성 세균에 대한 항균활성

Antimicrobial activity of honeybee venom against fish pathogenic bacteria

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In this study, we examined antimicrobial activity of the bee venom isolated from honeybee (Apis mellifera L.) against fish pathogenic bacteria, wardsiella tarda, Vibrio ichthyoenteri and Streptococcus iniae of cultured olive flounder, Paralichthys olvaceus. The bee venom exhibited significant antimicrobial activity against the both Gram-negative bacteria, E. tarda and V. ichthyoenteri and Gram-positive bacteria, S. iniae. Minimum inhibitory concentration (MIC) and minimun bactericidal concentration (MBC) of the bee venom were 17.6 ㎍/㎖, 34.6 ㎍/㎖ against E. tarda., and 1.76 ㎍/㎖, 6.8 ㎍/㎖ against V. ichthyoenteri, respectively. MIC and MBC of the bee venom were 3.49 ㎍/㎖, 11 ㎍/㎖, respectively against S. iniae The postantibiotic effect (PAE) of the bee venom was 5 hr, 6 hr, and 7 hr against E. tarda, V. ichthyoenteri, and S. iniae, respectively. In addition, its antimicrobial activity was stable under various pH conditions. According to these results, the bee venom showed the excellent antimicrobial activity against the tested pathogenic bacteria.

Abstract

In this study, we examined antimicrobial activity of the bee venom isolated from honeybee (Apis mellifera L.) against fish pathogenic bacteria, wardsiella tarda, Vibrio ichthyoenteri and Streptococcus iniae of cultured olive flounder, Paralichthys olvaceus. The bee venom exhibited significant antimicrobial activity against the both Gram-negative bacteria, E. tarda and V. ichthyoenteri and Gram-positive bacteria, S. iniae. Minimum inhibitory concentration (MIC) and minimun bactericidal concentration (MBC) of the bee venom were 17.6 ㎍/㎖, 34.6 ㎍/㎖ against E. tarda., and 1.76 ㎍/㎖, 6.8 ㎍/㎖ against V. ichthyoenteri, respectively. MIC and MBC of the bee venom were 3.49 ㎍/㎖, 11 ㎍/㎖, respectively against S. iniae The postantibiotic effect (PAE) of the bee venom was 5 hr, 6 hr, and 7 hr against E. tarda, V. ichthyoenteri, and S. iniae, respectively. In addition, its antimicrobial activity was stable under various pH conditions. According to these results, the bee venom showed the excellent antimicrobial activity against the tested pathogenic bacteria.

참고문헌 (30)

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