Multilocus sequence analysis of the genus Aliivibrio: Identification and phylogeny of Aliivibrio species isolated from cultured walleye pollock (Gadus chalcogrammus) in Korea
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
We performed MLSA (multilocus sequence analysis) and phenotypic characterization of Aliivibrio species isolated from walleye pollock (Gadus chalcogrammus) maintained in 3 different facilities of Gangwon Province, the east coast of Korea. Of 38 Aliivibrio species identified by 16S rDNA sequences, 12 strains were randomly selected and MLSA was conducted with 5 house-keeping genes (gapA, gyrB, pyrH, recA and rpoA) and 16S rDNA gene. Phylogenetic analysis and homology of the concatenated sequences (4,580 bp) with other Vibrionaceae genera revealed that 4 strains (GNGc16.1, YYGc16.1, YYGc16.2, GSGc18.1) were identified as Aliivibrio logei and one strain (GSGc16.1) as A. wodanis. One strain (GSGc17.1) was tentatively identified as A. logei, but needs further analysis because it did not belong to the same clade with A. logei type strain. 6 strains (GSGc17.2, GNGc16.2, GSGc16.2, GSGc17.3, GSGc18.2, GSGc17.4) need further investigation as potential novel species. Either phenotypic characterization or 16S rDNA sequence alone did not provide enough information for identification of Aliivibrio strains at the species level. A. logei and A. wodanis are generally known as non-pathogenic bacteria, but also known as opportunistic or secondary pathogens of cold water fishes. Cares should be taken to prevent potential outbreaks due to these bacteria, although there was no outbreaks during the sampling period.
Abstract
We performed MLSA (multilocus sequence analysis) and phenotypic characterization of Aliivibrio species isolated from walleye pollock (Gadus chalcogrammus) maintained in 3 different facilities of Gangwon Province, the east coast of Korea. Of 38 Aliivibrio species identified by 16S rDNA sequences, 12 strains were randomly selected and MLSA was conducted with 5 house-keeping genes (gapA, gyrB, pyrH, recA and rpoA) and 16S rDNA gene. Phylogenetic analysis and homology of the concatenated sequences (4,580 bp) with other Vibrionaceae genera revealed that 4 strains (GNGc16.1, YYGc16.1, YYGc16.2, GSGc18.1) were identified as Aliivibrio logei and one strain (GSGc16.1) as A. wodanis. One strain (GSGc17.1) was tentatively identified as A. logei, but needs further analysis because it did not belong to the same clade with A. logei type strain. 6 strains (GSGc17.2, GNGc16.2, GSGc16.2, GSGc17.3, GSGc18.2, GSGc17.4) need further investigation as potential novel species. Either phenotypic characterization or 16S rDNA sequence alone did not provide enough information for identification of Aliivibrio strains at the species level. A. logei and A. wodanis are generally known as non-pathogenic bacteria, but also known as opportunistic or secondary pathogens of cold water fishes. Cares should be taken to prevent potential outbreaks due to these bacteria, although there was no outbreaks during the sampling period.
참고문헌 (41)
- Abayneh, T., "Multi-locus sequence analysis (MLSA) of Edwardsiella tarda isolates from fish", Vet. Microbiol., 158, pp.367~375 (2012)
- Ast, J. C., "Photobacterium kishitanii sp. nov., a luminous ma-rine bacterium symbiotic with deep-sea fishes", Int. J. Syst. Evol. Microbiol, 57, pp.2073~2078 (2007)
- Ast, J. C., "Phylogenetic analysis of the lux operon distinguishes two evolutionarily distinct clades of Photobacterium leiognathi", Arch. Microbiol, 123(181), pp.352~336 (2004)
- Ast, J. C., "Multi-gene analysis reveals previously unrecognized phylogenetic diversity in Aliivibrio", Syst. Appl. Microbiol, 32, pp.379~386 (2009)
- Bang, S. S., "Phenotypic characterization of Photobacterium logei (sp. nov.), a species related to P. fischeri", Curr. Microbiol, 1, pp.285~288 (1978)
- Beaz-Hidalgo, R., "Aliivibrio finisterrensis sp. nov., isolated from Manila clam, Ruditapes philippinarum and emended description of the genus Aliivibrio", Int. J. Syst. Evol. Microbiol., 60, pp.223~228 (2010)
- Beijerinck, M. W., "Le Photobacterium luminosum, bacterie lumineuse de la Mer du Nord", Arch. Neerl. Sci. Exactes. Nat, 23, pp.401~405 (1889)
- Brunvold, L., "Characterisation of bacterial communities associated with early stages of intensively reared cod(Gadus morhua)using Denaturing Gradient Gel Electrophoresis(DGGE)", Aquaculture, 272, pp.319~327 (2007)
- Cano-Gomez, A., "Multilocus sequence analysis provides basis for fast and reliable identification of Vibrio harveyi-related species and reveals previous misidentification of important marine pathogens", Syst. Appl. Microbiol, 34, pp.561~565 (2011)
- Doi, H., "Vibrio japonicus sp. nov., a novel member of the Nereis clade in the genus Vibrio isolated from the coast of Japan", PLoS One, 12, pp.e0172164~ (2017)
- Dunlap, P. V., "Phylogenetic analysis of host-symbiont specificity and codivergence in bioluminescent symbioses", Cladistics, 23, pp.507~532 (2007)
- Egidius, E., "Vibrio salmonicida sp. nov., a new fish pathogen", Int. J. Syst. Bacteriol, 36, pp.518~520 (1986)
- Glaeser, S. P., "Multilocus sequence analysis(MLSA)in prokaryotic taxonomy", Syst. Appl. Microbiol, 38, pp.237~245 (2015)
- Hjerde, E., "Co-cultivation and transcriptome sequencing of two co-existing fish pathogens Moritella viscosa and Aliivibrio wodanis", BMC Genomics, 16, pp.447~ (2015)
- 전찬혁, "명태(Theragra chalcogramma) 종묘에서 분리된 Vibrio anguillarum의 생화학적, 분자생물학적 특성", 한국수산과학회지, 49(3), pp.359~366 (2016), 한국수산과학회
- Kang, S. K., "What caused the collapse of walleye pollock population in Korean waters?", KMI Int. J. Maritime Affairs and Fisheries, 7, pp.43~58 (2015)
- Kaeding, A. J., "Phylogenetic diversity & cosymbiosis in the bioluminescent symbioses of ‘‘Photobacterium mandapamensis’’", Appl. Environ. Microbiol, 73, pp.3173~3182 (2007)
- Karlsen, C., "Co-infection of Atlantic salmon(Salmo salar), by Moritella viscosa and Aliivibrio wodanis, development of disease and host colonization", Vet. Microbiol, 171, pp.112~121 (2014)
- Khider, M., "The alternative sigma factor RpoQ regulates colony morphology, biofilm formation and motility in the fish pathogen Aliivibrio salmonicida", BMC Microbiol, 18, pp.116~ (2018)
- Kimura, M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol, 16, pp.111~120 (1980)
- Klemetsen, T., "An Insight into the Aliivibrio genus. A comparative study on relationships and traits of species within the genus Aliivibrio", Master's, pp.2016
- Konopleva, M. N., "A combination of luxR1 and luxR2 genes activates Prpromoters of psychrophilic Aliivibrio logei lux-operon independently of chaperonin GroEL", ES and protease Lon at high concentrations of autoinducer, 2016, pp.473, 1158~1162
- Kumar, S., "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol. Biol. Evol., 33, pp.1870~1874 (2016)
- Labella, A. M., "Description of new and amended clades of the Genus Photobacterium", Microorganisms, 6, pp.24~ (2018)
- Lunder, T., "Phenotypic and genotypic characterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon (Salmo salar) with ‘‘winter ulcer’’", Int. J. Syst. Evol. Microbiol., 50, pp.427~450 (2000)
- Meyers, T. R., "Isolation of the North American strain of viral hemorrhagic septicemia virus(VHSV)associated with epizootic mortality in two new host species of Alaskan marine fish", Dis. Aquat. Org, 38, pp.81~86 (1999)
- Nam, U. H., "Monitoring of viruses in cultured walleye pollock Gadus chalcogrammmus", J. Fish Pathol, 30, pp.1~9 (2017)
- Reid, H. I., "Analysis of bacterial populations in the gut of developing cod larvae and identification of Vibrio logei, Vibrio anguillarum and Vibrio splendidus as pathogens of cod larvae", Aquaculture, 288, pp.36~43 (2009)
- Saitou, N., "The neighbor-Joining Method-a new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
- Sawabe, T., "Inferring the evolutionary history of vibrios by means of multilocus sequence analysis", J. Bacteriol, 189, pp.7932~7936 (2007)
- Seo, H. J., "Monitoring of viruses in wild walleye pollock (Gadus chalcogrammus)population in Korea", J. Fish Pathol, 31, pp.71~79 (2018)
- 서주영, "Naturally Collection and Development until Yolk Absorption of Domestic Walleye Pollock Theragra chalcogramma Fertilized Eggs and Larvae", 한국산학기술학회논문지, 18(1), pp.49~54 (2017), 한국산학기술학회[DOI]
- Soto, W., "Microbial experimental evolution as a novel research approach in the Vibrionaceae and squid-Vibrio symbiosis", Front. Microbiol, 5, pp.593~ (2014)
- Suh, S. S., "Seasonal dynamics of marine microbial community in the South Sea of Korea", PLoS One, 10, pp.e0131633~ (2015)
- Thompson, C. C., "Genomic taxonomy of Vibrios", BMC Evol. Biol, 9, pp.258~ (2009)
- Thompson, F. L., "Phylogeny and molecular identification of Vibrios on the basis of multilocus sequence analysis", Appl. Environ. Microbiol, 71, pp.5107~5115 (2005)
- Urbanczyk, H., "Reclassification of Vibrio fischeri, Vibrio logei, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Allivibrio logei comb. nov., Allivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov", Int. J. Syst. Evol. Microbiol, 57, pp.2823~2829 (2007)
- Wamala, S. P., "Multilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Uganda", J. Fish dis, 41, pp.1579~1600 (2018)
- Weisburg, W. G., "16S Ribosomal DNA amplification of phylogenetic study", J. Bacteriol, 173, pp.697~703 (1991)
- White, V. C., "Ichthyophonusinfected Walleye Pollock Theragra chalcogramma (Pallas) in the eastern Bering Sea: a potential reservoir of infections in the North Pacific", J. Fish Dis, 37, pp.641~655 (2014)
- Yoshizawa, S., "Aliivibrio sifiae sp. nov., luminous marine bacteria isolated from seawater", J. Gen. Appl. Microbiol., 56, pp.509~518 (2010)
