Marine birnavirus (MABV)에 대한 단클론 항체 생산
Production of monoclonal antibodies against marine birnavirus
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We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.
Abstract
We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.
참고문헌 (23)
- Banerjee, R., "Recent advances in nanoparticle-based lateral flow immunoassay as a pointof-care diagnostic tool for infectious agents and diseases", Analyst, 143, pp.1970~1996 (2018)
- Huang, X., "Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection : a review", Biosens. Bioelectron, 75, pp.166~180 (2016)
- Isshiki, T., "Distribution of marine birnavirus in cultured marine fish species from Kagawa Prefecture, Japan", J. Fish Dis, 27, pp.89~98 (2004)
- Jang, M.S., "The status of viral hemorrhagic septicemia (VHS) occurrence in olive flounder (Paralichthys olivaceus) farms of Korea", Ph.D., pp.2019
- Jeong, H. N., "Production of monoclonal antibodies against viral hemorrhagic septicemia virus(VHSV, genotype IVa)from olive flounder", J. Fish Pathol, 30, pp.149~154 (2017)
- 정성주, "Distribution of Marine Birnavirus in Cultured Olive Flounder Paralichthys olivaceus in Korea", The Journal of Microbiology, 46(3), pp.265~273 (2008), 한국미생물학회
- 김위식, "신경괴사증바이러스(nervous necrosis virus, RGNNV genotype)에 대한단클론 항체 생산", 한국수산과학회지, 51(3), pp.328~331 (2018), 한국수산과학회[DOI]
- 공경희, "VHS (viral hemorrhagic septicemia)의 원인병원체인 VHSV (genotype IVa)에 대한 단클론 항체 개발", 한국어병학회지, 32(2), pp.059~067 (2019), 한국어병학회
- Kusuda, R., "Characteristics of two virus strains isolated from young Japanese flounder", Suisanzoushoku, 37, pp.115~120 (1989)
- Laemmli, U. K, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4", Nature, 227, pp.680~685 (1970)
- Liddell, J.E., "A practical guide to monoclonal antibodies", pp.67~88 (1991), John Wiley and Sons, Inc.
- Munro, E.S., "Fish diseases and disorders, Volume 3: viral, bacterial and fungal infections", pp.1~65 (2006), CABI
- Nakajima, K., "Production of monoclonal antibodies against yellowtail ascites virus", J. Fish Dis, 19, pp.161~166 (1996)
- Oh, M.J., "Biological and serological characteristics of birnavirus isolated from cultured Japanese flounder in 1999", J. Fish Pathol., 12, pp.56~62 (1999)
- Oh, M. J., "Detection of birnavirus from cultured marine fish using polymerase chain reaction (PCR)", J. Fish Pathol., 12, pp.49~55 (1999)
- Oh, M. J., "The screening of marine birnavirus(MABV)infected in brood stocks of flounder, Paralichthys olivaceus", J. Fish Pathol, 13, pp.53~59 (2000)
- Oh, M. J., "Change of pathogenicity in olive flounder Paralichthys olivaceus by co-infection of Vivrio harveyi, Edwardsiella tarde and marine biravirus", Aquaculture, 257, pp.156~160 (2006)
- Park, S.C., "Production and characterization of monoclonal antibody against marine birnavirus", pp.2004
- Sohn, S. G., "Birnavirus isolated from cultured flounder in Korea", Fish Pathol, 30, pp.279~280 (1995)
- Sorimachi, M., "Characteristics and pathogenicity of a virus isolated from yellowtail fingerlings showing ascites", Fish Pathol, 19, pp.231~238 (1985)
- Towbin, H., "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; procedure and some applications", Proc. Natl. Acad. Sci, 76, pp.4350~4354 (1979)
- Wong, R, "Lateral flow immunoassay" (2008), Humana Press, Springer
- 윤현미, "남해안 자연산 어류에서 Marine birnavirus (MABV)의 검출", 한국양식학회지, 21(1), pp.13~18 (2008), 한국양식학회