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Differentiation of Three Scuticociliatosis Causing Species in Olive flounder (Paralichthys olivaceus)by Multiplex PCR

김기홍|이은혜|김성미
수산생명의학회 (Aquatic Life Medical Science)|2004.08|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제17권 제2호|pp. 145-149

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Abstract

The definitive identification of ciliate species by morphological characteristics relies on time-consuming and laborious staining techniques. Therefore, in this study, we discriminated 3 scuticociliatosis causing species - Pseudocohnilembus persalinus, Uronema marinum and Philasterides dicentrarchi - in cultured olive flounder by multiplex PCR. The multiplex PCR based on the species-specific amplification of small subunit ribosomal RNA (SS rRNA) gene sequence enabled us to distinguish the 3 scuticociliate species in a simple and rapid manner, even in the sample containing the three species simultaneously. These data suggest that the multiplex PCR strategy would make it possible to avoid the cumbersome and time-consuming procedures of morphological analysis for the definitive identification of scuticociliates.

참고문헌 (14)

  1. "Studies on the morphology of Uronema marinum Dujardin with a description of the histopathology of the infection in marine fishes", pp.295~303 (1980), Uronematidae
  2. "Surface immobilization antigens of Ichthyophthirius multifiliis: their role in protective immunity", 5, pp.113~131 (1995)
  3. "Philasterides dicentrarchi, n. sp. (Ciliophora, Scuticociliatida), a histophagous opportunistic parasite of Dicentrachus labrax (Linnaeus. 1758), a reared marine fish. Eur", 31, pp.327~340 (1995)
  4. "Philasterides dicentrarchi (Ciliophora, Scuticociliatida) as the causative agent of scuticociliatosis in farmed turbot Scophthalmus maximus in Galicia (NW Spain). Dis. Aquat. Org", 46, pp.47~55 (2001)
  5. "Antiprotozoals effective in vitro against the scuticociliate fish pathogen Philasterides dicentrarchi. Dis. Aquat. Org", 49, pp.191~197 (2002)
  6. "Philasterides dicentrarchi (Ciliophora: Scuticociliatida) expresses surface immobilization antigens that probably induce protective immune responses in turbot.", 126, pp.125~134 (2003)
  7. "Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus. Dis. Aquat. Org", 47, pp.49~55 (2001)
  8. "Pseudocohnilembus persalinus (Ciliophora: Scuticociitida) is an additional causing species of scuticociliatosis in olive flounder (Paralichthys olivaceus). Dis. Aquat. Org., (submitted" (2004)
  9. "Occurrence of scuticociliatosis in olive flounder (Paralichthys olivaceus) by Phiasterides dicentrarchi (Ciliophora: Scuticociliatida). Dis. Aquat. Org., (submitted" (2004)
  10. "Variation in primary sequence and tandem repeat copy number among i-antigens of Ichthyophthirius multifiliis", 120, pp.93~106 (2002)
  11. "Fatal encephalitis due to the scuticociliate Uronema nigricans in sea-caged, southern bluefin tuna Thunnus maccoyii. Dis. Aquat. Org.,", 30, pp.17~25 (1997)
  12. "Piperazine N-substituted naphthyridines, pyridothienopyrimidines and pyridothienotriazines: new antiprotozoals active against Philasterides dicentrarchi. Eur. J. Med. Chem.,", 38, pp.265~275 (2003)
  13. "a marine facultative parasite in the ciliate Order Hymenostomatida", pp.378~381 (1964)
  14. "Immunisation of channel catfish, Ictalurus punctatus, with Ichthyophthirius multifiliis immobilisation antigens elicits serotype-specific protection", 13, pp.337~350 (2002)

관련 논문AI추천

Neoparamoeba sp. infection on gills of olive flounder, Paralichthys olivaceus in Korea김기홍, 김형준, 조재범, 허민도, Mu-Kun Lee수산생명의학회 · 한국어병학회지2005 · 제18권 제2호제주지역 양식 넙치(Paralichthys olivaceus)를 대상으로 한 스쿠티카증(Scuticociliatosis) 모니터링고예진, 김예지, 오영은, 이영준, 이응준, 전려진, 정준범, 한지은수산생명의학회 · 한국어병학회지2023 · 제36권 제1호Proteases in Cell Lysate of Uronema marinum(Ciliata: Scuticociliatida), an Opportunistic Pathogen of Cultured Olive Flounder(Paralichthys olivaceus)ChunSooKim, HyungHoLee, JaeBumCho, JoonKiChung, KiHongKim, KyoungJinAhn, SeRyunKwon한국수산과학회 · Fisheries and Aquatic Sciences2002 · 제5권 제3호제주도 양식넙치, Paralichthys olivaceus의 스쿠티카충병 발생 동향송춘복, 오상필, 이제희, 이창훈, 정용욱, 진창남, 허문수수산생명의학회 · 한국어병학회지2003 · 제16권 제2호넙치 주요 세균성 병원체 4종의 동시 검출을 위한 Multiplex PCR 기법 개발강효영, 김도형, 김윤재, 박지연, 이윤항, 이주엽, 허영웅수산생명의학회 · 한국어병학회지2025 · 제38권 제2호Co-infection of two myxosporean parasites - Parvicapsula anisocaudata and an unidentified myxosporean - in the kidney of cultured olive flounder, Paralichthys olivaceus김기홍, 조재범, 허민도, Mu-Kun Lee수산생명의학회 · 한국어병학회지2005 · 제18권 제2호

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