해수 양식 어류에서 분리된 연쇄상구균의 종류와 병원성
Pathogenicity and classification of streptococci isolated from cultured marine fishes
2
조회
0
다운로드
18
인용
0
참고문헌
초록
우리 나라의 해수 양식 어류에서 연쇄구균병의 증상을 나타내는 병어로부터 분리한 원인균의 종 분포를 조사하고 그 특성을 연구하고자 Streptococcus parauberis, Lactococcus garvieae 및 Streptococcus iniae에 대한 PCR법 종 동정 결과를 토대로 각 종의 미생물학적 특성을 비교 분석하였다. 혈청학적 특성에서는 S. parauberis가 강한 종 특이성을 나타내었다. L. garvieae는 capsule 염색과 TEM을 통한 형태학적 연구를 통해서 KG＋와 KG－ phenotype cell의 구별이 가능하였으며, S. iniae 대표 균주들은 capsule-like material의 존재를 확인할 수 없었다. 본 실험에 사용된 시험 균주 중 S. parauberis와 L. garvieae는 모두 non-hemolytic 이었지만, S. iniae는 모두 β-hemolytic한 특징을 나타내었다. 시험균의 넙치와 조피볼락에 대한 병원성 시험 결과, S. iniae가 넙치와 조피볼락에 대하여 급성 또는 만성적으로 병원성을 나타내었다. L. garvieae의 두 phenotype이 모두 넙치와 조피볼락에 대해 2주째까지 지느러미 발적 등의 증상을 나타내었으나 폐사 개체는 관찰되지 않았다.
Abstract
Thirty-five streptococcci were isolated from the diseased streptoccoccicosis in oliver flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli) and yellowtail (Seriola quinqueradiata). The isolates were identified into 3 kinds of streptococccal species, Streptococcus parauberis, Lactococcus garvieae and Streptococcus iniae by PCR assay with specific primers, pSP-1, pSP-2, pLG-1, pLG-2, pSI-1, pSI-2. Several characteristics were investigated on the morphological, biochemical, physiological and serological tests. The isolates were constituted as 5 strains of S. parauberis, 11 strains of L. garvieae and 19 strains of S. iniae. L. garvieae and S. parauberis strains appeared non-hemolytic whereas St. iniae showed β-hemolysis . In the serological investigation, S. parauberis antigen of formalin killed cells (FKCs) exhibited specificity against all the tested antisera. The antisera of L. garvieae (KG－ phenotype) and the S. iniae showed cross-reaction against to the FKCs of L. garvieae (KG＋ phenotype). Five out of 11 strains of L. garvieae were identified as KG－ phenotype with serological test and by TEM observation, although S. iniae could not be observed the capsule structure. In the pathogenicity test with L. garvieae and S. iniae to the oliver flounder and the black rockfish, S. iniae induced acute or chronic mortality in both fishes, whereas L. garvieae caused hemorrhage at the base of fins of tested fishes without any mortality.
참고문헌 (24)
- "Comparative pathogenicity study on antigenically variant strains of Enterococcus seriolicida", 19, pp.39~46 (1996)
- "Trout mortalities as a result of Streptococcus infection", 46, pp.71~78 (1979)
- "Histopathology of experimental Streptococcus sp. infection in tilapia, Oreochromis niloticus(L.), and channel catfish, Ictalurus punctatus(Rafinesque).", 19, pp.235~241 (1996)
- "Manual for the identification of medical bacteria" (1993), Cambridge University Press
- "Biodiversity of Lactococcus garvieae strains isolated from fish in Europe", 65, pp.1005~8 (1999)
- "A Streptococcus pathogenic to fish", 16, pp.201~206 (1958)
- "Epidemiologic study on streptococcicosis of cultured yellowtail Distribution of Streptococcus sp in sea water and muds around yellowtail farms", pp.567~572 (1979), Seriola quinqueradiata
- "Epizootic caused by β-hemolytic Streptococcus species in cultuerd freshwater fish", pp.301~307 (1981)
- "Strain variation associated with pathogenesis of Streptococcus sp the causative bacteria of streptococcicosis in cultured yellowtail", Seriola quinqueradiata Tokai University
- "A new pathogenic bacterium belonging to the genus Streptococcus isolated from an epizootic of cultured yellowtail", pp.1345~1352 (1976)
- "A comparative study of fish pathologenic Streptococcus isolated from saltwater and freshwater fisheries", pp.1073~1078 (1978)
- "Biochemical test for identification of medical bacteria" (2000), Lippincott Willams and Wilkins, Third edition,
- "pathogenic to cultured yellowtail isolated from fisheries for diets", pp.15~19 (1979)
- "A streptococcal disease of cultured yellowtail", pp.281~285 (1983)
- "Experimental Streptococcus iniae infection in Japanese flounder(Paralichthys olivaceus).", 36, pp.40~41 (2001)
- "The protective immune response of yellowtail Seriola quinqueradiata to the bacterial fish pathogen Lactococcus garvieae", 37, pp.121~126 (1999)
- "a β-hemolytic Streptococcus isolated from an Amazon freshwater dolphin International Journal of Systematic Bacteriology", (v), pp.543~553 (1976)
- "Health maintenance of cultured fishes", pp.231~235 (1994), Principal microbial diseases CRC Press
- "Pathogenicity of a non-hemolytic group B Streptococcus sp", pp.97~37 105 (1984)
- "Role of the cell envelope in bacterial adaptation to growth in vivo in infections", 70, pp.987~1011 (1998)
- "Phenotypic variation associated with an anti-phagocytic factor in the bacterial fish pathogen Enterococus seriolicida", 25, pp.81~86 (1996)
- "Associated of the cell capsule with anti-opsonophagocytosis in β-hemolytic Streptococcus spp. isolated from rainbow trout.", 8, pp.223~228 (1996)
- "A cell capsule with possible involvement in resistance to opsonophagocytosis in Enterococcus seriolicida from yellowtail , Seriola quinqueradiata.", 29, pp.233~235 (1997)
- "Possible transmission of Streptococcus iniae from wild fish to cultured marine fish", 64, pp.4065~4067 (1998)