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항바이러스 활성 유도 물질에 대한 잉어의 선청성 면역 반응

Innate immune responses of common carp, Cyprinus carpio L. against antiviral activity inducers

김진우|김수미|박수일|조미영|김은전|손상규
수산생명의학회 (Aquatic Life Medical Science)|2007.08|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제20권 제2호|pp. 189-200

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초록

잉어의 선천성 면역 인자가 관여하는 항바이러스 면역 반응을 조사하기 위해 UV-inactivated SVCV, Poly I:C 및 Con A를 주사한 후 3일째 라이소자임 활성, 혈청 내 보체의 살균능력 및 식세포의 활성산소량을 조사하였다. 그 결과, 모든 시험구에서 혈청 내 라이소자임의 활성은 유의적인 차이를 나타나지 않았으나, 두신 조직의 라이소자임 활성은 대조구에 비해 유의적으로 증가하였다. 또한, 혈청 내 보체의 살균 능력도 모든 시험구에서 대조구와 유의적인 차이가 없었다. 그러나 식세포의 활성은 UV-inactivated SVCV 시험구에서는 농도에 따라 증가한 것으로 나타났으며, Poly I:C 및 Con A 시험구에서는 저농도에서 활성이 증가한 것으로 나타났다. 바이러스에 대한 방어력을 조사하기 위해 주사 후 4일째 1×104 TCID50/fish 농도의 SVCV로 인위 감염한 결과 UV-inactivated SVCV 및 Poly I:C 시험구에서는 Con A 시험구에 비해 높은 방어력을 나타내었다. 또한, Poly I:C 시험구에서 라이소자임 및 식세포 활성이 다소 감소한 고농도에서도 높은 방어력이 유도된 것으로 나타나 이러한 결과는 Poly I:C에 의해 자극된 또 다른 비특이적 면역 인자가 SVCV에 대한 방어반응에 관여한 것으로 추정된다.

Abstract

To investigate the innate immune response involved in early stage of anti-viral defence, carps were injected with UV-inactivated spring viraemia of carp virus (SVCV), poly inosinic:cytidylic acid (Poly I:C) and concanavalin A (Con A), respectively and examined lysozyme activity, serum complement activity and chemiluminescent (CL) response of leucocytes isolated from head kidney at 3 days post-injection. There was no significant difference in plasma lysozyme activities among all experimental groups. However, lysozyme activities of head kidney in the groups injected with antiviral activity inducers were significantly higher than those of the control injected with physiological saline. Bactericidal activities of serum of the groups injected with antiviral activity inducers were not significantly different from control group. However, the CL responses were significantly higher at lower dose of Poly I:C and Con A, whilst dose-dependent increase was shown in UV-inactivated SVCV-injected group. In the challenge test with 1×104 TCID50/fish of SVCV at 4 days post-injection, UV-inactivated SVCV- and Poly I:C-injected groups showed higher relative percent survival (RPS) than Con A-injected group. Furthermore, strong protection was observed in the group injected higher dose of Poly I:C although showed lower activities in lysozyme and CL response. These results suggested that Poly I:C might stimulate other factors belonging to non-specific immune system have induced protective immunity against the SVCV challenged.

참고문헌 (45)

  1. "The influence of environmental temperature and infection route on the immune response of carp to spring viremia of carp virus", pp.383~386 (1986)
  2. "Noncellular nonspecific defence mechanisms of fish. Ann. Rev. Fish Dis.", pp.249~280 (1992)
  3. "Innate antiviral defenses in body fluids and tissues", 48, pp.71~89 (2000)
  4. "Host-virus relationship during experimental spring viremia in carp. C. R. Acad. Sci. Hebd. Seances Acad. Sci. D.", pp.1225~1228 (1978)
  5. "Complement dependent mechanisms of virus neutralization. In Immunology of the Complement System. Academic Press", pp.139~162 (1986)
  6. "Heterogeneity of mitogen-responsive lymphocytes in carp (Cyprinus carpio)", 8, pp.61~70 (2002)
  7. "Pathogenesis and immune response in Atlantic salmon (Salmo salar L.) parr experimentally infected with salmon pancreas disease virus (SPDV)", 12, pp.77~95 (2002)
  8. "Nonspecific defenses", pp.631~640 (1996), Galveston, Tx
  9. "The relationship between major histocompatibility receptors and innate immunity in teleost fish", 25, pp.683~699 (2001)
  10. "Anti-viral activity in four species of salmonids following exposure to poly inosinic"
  11. "Lysozyme assay. In Techniques in Fish Immunology", pp.101~103 (1990)
  12. "Vaccination of carp against spring viremia comparison of intraperitoneal and peroral application of live virus to fish kept in ponds. Bull. Off. Epiz.", pp.1055~1068 (1977)
  13. "The lysozyme of the plaice Pleuronecyes platessa L. Comp. Biochem. Physiol.", pp.207~210 (1976)
  14. "Enhancement of viral RNA-induced interferon production in L cells treated with insulin and Amphotericin B methyl ester. J. Interferon Res.", pp.581~586 (1981)
  15. "Studies on Poly I C toxicity in experimental animals. Toxicol. App. Phamacol.", pp.579~588 (1972)
  16. "IFN induction and associated changes in splenic leukocyte distribution. J. Immunol.", pp.3713~3727 (1993)
  17. "Effects of Poly I:C on the expressions and resistance against infection by infectious salmon anemia virus in Atlantic salmon", 13, pp.311~326 (2002)
  18. "What's new in lysozyme research? Always model system today as yesterday. Mol. Biochem.", pp.165~189 (1984)
  19. "Involvement of the IRF-1 transcription factor in antiviral responses to interferons. Science" (1921)
  20. "potent inducer of interferons in promates. J. Interferon Res.", pp.539~549 (1981)
  21. "Optimization and kinetics of in vitro stimulation of carp (Cyprinus carpio L.) leucocytes", 3, pp.325~343 (2002)
  22. "Complement does not mediated the febril responses of guinea pigs to muramyl depeptide and polyriboinosinic-polyribocytidylic acid", 25, pp.51~58 (2000)
  23. "Poly I:C-induced Mx responses in Atlantic salmon parr, post-smolts and growers", 17, pp.245~254 (2004)
  24. "Immunity to VHS virus in rainbow trout", 172, pp.41~61 (1999)
  25. "Polyinosinic acid. poly cytidylic acid promotes T helper typeⅠ-specific immune responses by stimulating macrophage production of interferon-alpha and interleukin-12", 25, pp.2656~2660 (1995)
  26. "Comparative antiviral effects of dsRNA on lower and higher vertebrates", 26, pp.536~538 (1995)
  27. "Vaccination of carp against spring viremia with inactivated vaccine. In Ichthyopathology in Aquaculture" (1988)
  28. "The estimate of the bacterial power of blood. J. Hygiene", pp.873~885 (1938)
  29. "Phase Ⅰ-Ⅱ trials of poly", pp.1~4 (1982)
  30. "In vivo effects of β-glucan and LPS on regulation of lysozyme activity and mRNA expression in Atlantic salmon (Salmo salar L.)", 12, pp.1~16 (2002)
  31. "Systemic toxicity of polyinosinic acid polycytidylic acid in rodents and dogs. Toxicol. App. Pharmacol. 18", pp.220~230 (1971)
  32. "A novel autoimmune pancreatitis model in MRL mice treated with polyinosinic:polycytidylic acid", 129, pp.27~34 (2002)
  33. "Differential regulation of gene expression during macrophage activation with a polyribonucleotide. The role of endogenously derived IFN. J. Immunol."
  34. "Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio", 21, pp.4677~4684 (2003)
  35. "Analysis of expressed tags (EST) obtained from common carp, Cyprinus carpio L., head kidney cells after stimulation by two mitogens, lipopolysaccharide and concanavalin-A", 131, pp.71~82 (2002)
  36. "Daily handling stress reduces resistance of carp to Trypanoplasma borreli: in vitro modulatory effects of cortisol on leukocyte function and apoptosis", 27, pp.233~245 (2003)
  37. "Complement and innate immunity", 49, pp.187~198 (2000)
  38. "Immunocytochemical localization of lysozyme in intestinal eosinophilic granular cells (EGCs) of Atlantic salmon, Salmo salar L", 19, pp.349~355 (1996)
  39. "Spring viremia of carp-development of a vaccine in Czechoslovakia. In Ichthyopathology in Aquaculture" (1988)
  40. "Identification of differentially expressed genes in ConA-activated carp (Cyprinus carpio L.) leucocytes", 124, pp.41~50 (1999)
  41. "이스라엘 잉어의 보체 활성에 미치는 스트레스의 영향", pp.11~24 (1995)
  42. "구기자 (Lycii fructus) 추출물이 나일틸라피아 (Oreochromis niloticus)의 면역반응에 미치는 효과", pp.29~ (1999)
  43. "Mitogen 투여에 대한 잉어 순환혈액 림프구의 반응", 9, pp.95~109 (1996)
  44. "잉어바이러스성신경괴사증바이러스 (VSNCV) 백신투여에 대한 잉어의 면역반응", 16, pp.175~181 (2003)
  45. "잉어 두신 백혈구에서 생성된 Interferon-like cytokine (ILC)의 항바이러스 활성", 17, pp.75~82 (2004)

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