Expressed Sequence Tags Analysis of Black Rockfish (Sebastes schlegeli) Peripheral Leukocytes Stimulated with Con A/PMA or LPS
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We constructed a black rockfish (Sebastes schlegeli) leukocyte cDNA library and a total of 386 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 386 EST clones, 199 different ESTs showed significant homology to previously described genes while 97 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 38 different sequences were identified as putative bio-defense genes or genes associated with immune response.
Abstract
We constructed a black rockfish (Sebastes schlegeli) leukocyte cDNA library and a total of 386 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 386 EST clones, 199 different ESTs showed significant homology to previously described genes while 97 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 38 different sequences were identified as putative bio-defense genes or genes associated with immune response.
참고문헌 (15)
- Azam, A., "Identification of novel genes from Entamoeba histolytica by expressed sequence tag analysis", Gene, 181, pp.113~116 (1996)
- Clem, L.W., "Temperature-mediated processes in teleost immunity: Differential effects of in vitro and in vivo temperatures on mitogenic responses of channel catfish lymphocytes", Dev. Comp. Immunol, 8, pp.313~322 (1984)
- Franco, G.R., "Identification of new Schistosoma mansonigenes by the EST strategy using a directional cDNA library", Gene, pp.141~147 (1995)
- Gish, W., "Identification of protein coding regions by database similarity search", Nature Genetics, 3, pp.266~272 (1993)
- Graham, S., "The production of a macrophage-activating factor from rainbow trout Salmo gairdneri leucocytes", Immunol, 65, pp.293~297 (1988)
- Johnson, M.D., "Molecular cloning of gene sequences regulated by tumor promoters and mitogens through protein kinase C.", Mol. Cell. Biol, 7, pp.2821~2829 (1987)
- Lee, C.K., "Geneexpression profile of the aging skin in mice", Nat. Genet, 25, pp.294~297 (2000)
- Manning, M.J., "Fishes. In 'Immunology: A comparative approach", pp.69~100 (1994), Chichester: John Wiley and Sons
- Miyamoto, S., "Signal transduction pathways that contribute to increased protein synthesis during T-cell activation", Biochimica. Biophysica. Acta, 1494, pp.28~42 (2000)
- Murphy, J.J., "Phorbol ester induction of early response gene expression in lymphocytic leukemia and normal human B cells", Leukoc. Res, 17, pp.657~662 (1993)
- Nam, B.H., "A survey of expressed genes in Japanese flounder, Paralichthys olivaceus, infected with Hirame rhabdovirus", Dev. Comp. Immunol, 24, pp.13~24 (2000)
- Siwicki, A.K., "In vitro effects of bhydroxy- b-methylbutyrate (HMB) on cellmediated immunity in fish", Vet. Immunol. Immunopathol, 76, pp.191~197 (2000)
- Takeshita, T., "Phorbol esters can persistently replace interleukin- 2 (IL-2) for the growth of a human IL-2-dependent T-cell line", J. Cell. Physiol, 136, pp.319~325 (1988)
- Ton, C., "Identification, characterization, and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library", Genome Res, 10, pp.1915~1927 (2000)
- Yin, Z., "Identification of deferentially expressed genes in Con A-activated Carp (Cyprinus carpio L.) leucocytes", Comp. Biochem. Physiol. Part B, 124, pp.41~50 (1999)