양식산 황복에서 tetrodotoxin 유사 독소의 미량 존재 가능성 제시
Possible existence of tetrodotoxin-like toxins in cultured river puffer fish, Takifugu obscurus
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양식산 복어에서는 tetrodotoxin(TTX)가 존재 하지 않는다고 일반적으로 생각되고 있다. 본 연 구에서는 실제로 양식산 황복(Takifugu obscurus) 에는 TTX가 존재하지 않는지 확인하기 위해 생 쥐를 이용한 bioassay로 여러 장기를 동시에 분 석하였다. 자연산에서는 동물이 치사하는 수준 의 독력을 함유한 시료가 있었으며 정소>간장> 장>근육>피부의 순으로 독력이 확인되었다.양 식산 시료에서는 TTX의 미량존재로 추정되는 졸림, 행동둔화, 후지마비 등의 증상이 나타났지 만 치사동물은 발견되지 않았다. 이 결과는 양식 산 황복에서 TTX가 매우 낮은 농도로 존재할 가능성을 제시하지만 기기분석법을 이용한 정 확한 정량이 필요하다고 판단된다.
Abstract
It was examined whether the common belief that "cultured puffer fishes do not contain tetrodotoxin (TTX)", the major lethal substance that accidently causes death in consumers of those fishes, is true in river puffer fish Takifugu obscurus. In mouse bioassay, lethal levels of toxins were detected in the ranks: gonad>liver>intestine>muscle>skin in wild puffer fish. In contrast, no mortality occurred in the mouse bioassay on cultured fish. However, there were sleepiness, sluggish behavior, and hind limb paralysis with the tissue extracts of cultured fish suggesting the presence of TTX or other similarly acting toxins. An attempt to confirm the presence of TTX in cultured fish with high performance liquid chromatography (HPLC) was not very successful. The results suggest possible existence of TTX toxins or similarly acting toxins.
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