넙치(Paralichthys olivaceus) 구강에 기생하는 Neoheterobothrium hirame의 형태 및 감염어의 병리조직
Morphology of Neoheterobothrium hirame parasitic in the oral cavity of olive flounder (Paralichthys olivaceus) and histopathology of diseased fish
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A disease that manifested severe anemia of the gills occurred in the olive flounder (Paralichthys olivaceus), which was cultured for 5-6 months with a recirculation water system in the laboratory. Microscopic observations showed immature parasite in the gills and mature adults in the oral cavity. The matured parasite was 5.60-9.32 (7.42) mm in total length, with 4 pairs of clamps on the pedunculated haptor, which was separated from the body proper by a long isthmus. From the morphological observations of the larvae, it was identified as Neoheterobothirum hirame belonging to the monogenea. The average hematocrite of infected fish was 10.3 ± 2.8%, significantly lower than that of normal flounder 31.4 ± 4.2%. Histopathologically, fish infected with N. hirame exhibited reduced numbers of erythrocytes in blood vessels of the gill filament and lamellae, reduced red pulp area of the spleen, and hepatocyte atrophy. From the above results, the cause of severe anemia in olive flounder was identified as N. hirame infection. This study shows an example where the growth of pathogens can be a problem in the recirculation system due to low water exchange rate.
Abstract
A disease that manifested severe anemia of the gills occurred in the olive flounder (Paralichthys olivaceus), which was cultured for 5-6 months with a recirculation water system in the laboratory. Microscopic observations showed immature parasite in the gills and mature adults in the oral cavity. The matured parasite was 5.60-9.32 (7.42) mm in total length, with 4 pairs of clamps on the pedunculated haptor, which was separated from the body proper by a long isthmus. From the morphological observations of the larvae, it was identified as Neoheterobothirum hirame belonging to the monogenea. The average hematocrite of infected fish was 10.3 ± 2.8%, significantly lower than that of normal flounder 31.4 ± 4.2%. Histopathologically, fish infected with N. hirame exhibited reduced numbers of erythrocytes in blood vessels of the gill filament and lamellae, reduced red pulp area of the spleen, and hepatocyte atrophy. From the above results, the cause of severe anemia in olive flounder was identified as N. hirame infection. This study shows an example where the growth of pathogens can be a problem in the recirculation system due to low water exchange rate.
참고문헌 (24)
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