수입 냉동새우에서 검출된 WSSV의 유전학적 근연관계 조사
Genetic relatedness of white spot syndrome virus (WSSV) from imported frozen shrimp
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초록
본 연구에서는 주요 새우 생산 국가에서 2017년 7월부터 2020년 11월 사이 생산되어 국내로 수입된 냉동 새우(29개 그룹)를 대상으로 흰반점바이러스(WSSV), covert mortality nodavirus (CMNV) 및 decapod iridescent virus 1 (DIV-1)의 검출여부를 조사하였다. 각 바이러스에 대한 nested PCR 결과, WSSV는 9개 그룹(9/29)에서 검출되었으며 CMNV와 DIV-1은 검출되지 않았다. Nested PCR에서 WSSV 양성으로 확인된 시료를 대상으로 WSSV genome variable loci로 알려진 VR 14/15 region에 대해 참조 서열들과 삽입/결손(insertion and deletion) 서열 비교 및 근연관계를 분석하였다. WSSV 양성 시료 중 1개 시료(20-CH-1 isolate, 2020년 10월 중국 생산)에서만 VR 14/15에 대한 PCR amplicon이 생성되었으며 염기서열 분석 결과, 20-CH-1 isolate는 2005년 인도에서 보고된 WSSV-IN-05-01과 99.84%의 상동성을 보였다. 이는 과거 알려진 바와 같이 새우의 교역을 통한 국가 간 WSSV가 확산되었음을 뒷받침해주는 결과이다.
Abstract
In this study, of the imported shrimps between 2017 and 2020, we investigated white spot syndrome virus (WSSV), covert mortality nodavirus (CMNV) and decapod iridescent virus 1 (DIV-1). Of the imported shrimps (a total of 29 groups), WSSV was detected as 31% (9/29) by nested PCR assay. And CMNV and DIV-1 were not identified in this study. To investigate the genetic relatedness of WSSV identified from imported shrimp, VR 14/15 region showed WSSV genomic variable loci was compared with reference isolates. Among the nine WSSV-positive samples, VR 14/15 region was amplified in only a sample (20-CH-1 isolate, imported from China in 2020). And the 20-CH-1 isolate showed 99.8% identity with WSSV-IN-05-01 which was reported in India in 2005, suggesting that those of WSSV have been spread from India to China. Furthermore, although the pathogenicity of WSSV identified from frozen shrimp was not evaluated, the international trade of diseased frozen shrimps could be led to the potential risk of virus transmission.
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