Vibrio parahaemolyticus와 V. vulnificus 사람 분리균의 넙치, Paralichthys olivaceus에서의 생존율
Viability of Vibrio parahaemolyticus and V. vulnificus isolated from human in cultured olive flounder (Paralichthys olivaceus)
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Vibrio parahaemolyticus and V. vulnificus are known to be infected to human via fisheries products. Therefore, food safety of fisheries products is important for public health and fish industry. This paper was conducted to know how well these human isolates can survive in olive flounder (Paralichthys olivaceus). The growth of V. parahaemolyticus and V. vulnificus showed about 50∼60% reduced rates at 25℃ than at 37℃ and did not show any differences according to NaCl concentration of media except the increasing in the growth of V. vulnificus in medium containing 3% NaCl. Artificial infection of 1×106 CFU/fish was carried out to confirm the sensitivity of olive flounder against V. parahaemolyticus and V. vulnificus. After 1 week from injection, no fish was dead. To evaluate nonspecific defense of olive flounder against V. parahaemolyticus and V. vulnificus, the antibacterial potency of serum and epidermal mucus were tested. The number of the vibrios exposed to serum obtained from olive flounder significantly decreased after 3 hours, and epidermal mucus showed decrease of the bacteria over than 90% until 12 hours from exposure. Phagocytosis of head kidney leucocytes of healthy olive flounder against V. parahaemolyticus and V. vulnificus showed in over 70% of leucocytes at the 2 hours. Therefore, cultured olive flounder only as vehicle for human pathogen in environmental water is well developed its antibacterial potency against human pathogens, so the viability of V. parahaemolyticus and V. vulnificus in cultured olive flounder was considered very low.
Abstract
Vibrio parahaemolyticus and V. vulnificus are known to be infected to human via fisheries products. Therefore, food safety of fisheries products is important for public health and fish industry. This paper was conducted to know how well these human isolates can survive in olive flounder (Paralichthys olivaceus). The growth of V. parahaemolyticus and V. vulnificus showed about 50∼60% reduced rates at 25℃ than at 37℃ and did not show any differences according to NaCl concentration of media except the increasing in the growth of V. vulnificus in medium containing 3% NaCl. Artificial infection of 1×106 CFU/fish was carried out to confirm the sensitivity of olive flounder against V. parahaemolyticus and V. vulnificus. After 1 week from injection, no fish was dead. To evaluate nonspecific defense of olive flounder against V. parahaemolyticus and V. vulnificus, the antibacterial potency of serum and epidermal mucus were tested. The number of the vibrios exposed to serum obtained from olive flounder significantly decreased after 3 hours, and epidermal mucus showed decrease of the bacteria over than 90% until 12 hours from exposure. Phagocytosis of head kidney leucocytes of healthy olive flounder against V. parahaemolyticus and V. vulnificus showed in over 70% of leucocytes at the 2 hours. Therefore, cultured olive flounder only as vehicle for human pathogen in environmental water is well developed its antibacterial potency against human pathogens, so the viability of V. parahaemolyticus and V. vulnificus in cultured olive flounder was considered very low.
참고문헌 (20)
- Aranishi, F., "Response of skin cathepsins to infection of Edwardsiella tarda in Japanese flounder", Fisheries Sci, 66, pp.169~170 (2000)
- Dash, S., "Epider-mal mucus, a major determinant in fish health : a review", IJVR, 19, pp.72~81 (2018)
- Deeb, R., "Impact of climate change on Vibrio vulnificus abundance and exposure risk", Estuaries and Coasts, 41, pp.2289~2303 (2018)
- Findlay, V. L., "The immunomodulatory effects of levamisole on the nonspecific immune system of Atlantic salmon, Salmon salar L.", J. Fish Dis, 234, pp.369~378 (2000)
- Foua, B., "Susceptibility of Nile tilapia(Oreochromis niloticus)to vi-briosis due to Vibrio vulnificus biotype 2(serovar E)", Aquaculture, 212, pp.21~30 (2002)
- Gu, H., "Macrophage col-ony stimulating factor(MCSF)of Japanese flounder(Paralichthys olivaceus) : Immunoregulatory property, anti-infectious function, and interaction with MCSF receptor", Develop., and Com. Immunol, 116, pp.103920~ (2021)
- 정영일, "2016년도 국내 해양환경내 병원성 비브리오균의 분포 및 해양환경인자간의 상관성 분석", 한국환경보건학회지, 44(2), pp.133~142 (2018), 한국환경보건학회
- "Epidemiological investigation of infectious disease in Republic of Korea annual report" (2019)[URL]
- "Distribution of pathogenic vibrios in the aquatic environment adjacent to coastal areas of South Korea" (2019)[URL]
- "Infectious disease surveillance yearbook" (2020)[URL]
- Linkous, D. A., "Pathogenesis of Vibrio vulnificus", FEMS Microbiol. Letter, 174, pp.207~214 (1999)
- Le Roux, F., "The emergence of Vibrio pathogens in Europe: ecology, evolution, and pathogenesis (Paris, 11-12th March 2015)", Front. Microbiol, 830(6) (2015)[DOI]
- Leung, K. Y., "Serum resistance as a good indicator for virulence in Aero-monas hydrophila strains isolated from diseased fish in South-East Asia", J. Fish Dis, 18, pp.511~518 (1995)
- Mathews, E. S., "Assays of immune function in fish macrophages. In Techni- ques in Fish Immunology", pp.155~163 (1990), SOS Publications
- 박성우, "어류 혈액 염색에 Diff-Quick kit의 적용 가능성 검토", 한국어병학회지, 17(2), pp.151~157 (2004), 한국어병학회
- Sakazaki, R., "Studies in the enteropathogenic, facultatively halophilic bacteria. Vibrio parahaemolyticus. Ⅰ. Morphological, cultural and biochemical properties and its taxonomical position", Jap. J. Med. Sci. Biol., 16, pp.161~188 (1963)
- Secombes, C.J., "The nonspecific immune system; cellular defenses. In The fish immune system; Organism, Pathogen and Environment", pp.63~103 (1996), Academic press
- Song, M. K., "Study on the distribution of Vibrio parahaemolyticus along Cheju coast", Korean J. Environ. Biol, 25, pp.34~41 (2007)
- Suhalim, R. R., "Interaction of Escherichia coli O157 : H7 E318 cells with the mu-cus of harvested channel catfish(Ictalurus punctatus)", LWT-Food Sci. Technol, 40, pp.1266~1270 (2007)
- Xu, F., "Genetic characterization of clinical and environmental Vibrio parahaemolyticus from the Northeast USA reveals emerging resident and non-in- digenous pathogen lineages", Front. Microbiol, 272(6) (2015)