넙치 유래 VHSV (Viral haemorrhagic septicemia virus)의 살균방법별 저감효과 연구
Comparision study on disinfection systems for decrease in viral haemorrhagic septicemia virus (VHSV) of olive flounder (Paralichthys olivaceus)
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초록
Viral haemorrhagic septicemia virus (VHSV) is mainly transmitted horizontally by water-borne transmission. To prevent the spread of infectious VHSV in the aquatic environment, the water treatment is needed in aquaculture system. Although several studies have investigated to reduction of VHSV titer using the water treatment system, comparing of viral titer reduction in seawater by using different types of disinfection system has not been studied yet. Here, we determined the disinfectant effect of VHSV in seawater by using 4 types (UV radiation, plasma-AOP, electrolysis, ozonation) disinfection system. Moreover, cumulative mortality and detection rate of VHSV in olive flounder (Paralichthys olivaceus) after cohabitation challenges was also investigated viral reduction by disinfection system (UV radiation or plasma-AOP) or by untreated system. VHSV copy numbers were decreased from 105.54 copies/mL to 102.17±0.53 copies/mL, 102.31±0.22 copies/mL, 102.44±0.27 copies/mL, and 103.14±0.11 copies/mL by the UV radiation, plasma-AOP, electrolysis, ozonation, respectively. In cohabitation challenge, the cumulative mortality and VHSV detection rate of olive flounder by the plasma-AOP treatment is lower than that by the UV radiation treatment and positive control. These results suggest that the plasma-AOP treatment system can be useful for efficient inactivation of VHSV.
Abstract
Viral haemorrhagic septicemia virus (VHSV) is mainly transmitted horizontally by water-borne transmission. To prevent the spread of infectious VHSV in the aquatic environment, the water treatment is needed in aquaculture system. Although several studies have investigated to reduction of VHSV titer using the water treatment system, comparing of viral titer reduction in seawater by using different types of disinfection system has not been studied yet. Here, we determined the disinfectant effect of VHSV in seawater by using 4 types (UV radiation, plasma-AOP, electrolysis, ozonation) disinfection system. Moreover, cumulative mortality and detection rate of VHSV in olive flounder (Paralichthys olivaceus) after cohabitation challenges was also investigated viral reduction by disinfection system (UV radiation or plasma-AOP) or by untreated system. VHSV copy numbers were decreased from 105.54 copies/mL to 102.17±0.53 copies/mL, 102.31±0.22 copies/mL, 102.44±0.27 copies/mL, and 103.14±0.11 copies/mL by the UV radiation, plasma-AOP, electrolysis, ozonation, respectively. In cohabitation challenge, the cumulative mortality and VHSV detection rate of olive flounder by the plasma-AOP treatment is lower than that by the UV radiation treatment and positive control. These results suggest that the plasma-AOP treatment system can be useful for efficient inactivation of VHSV.
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