Endosymbionts and Phage WO Infections in Korean ant Species (Hymenoptera: Formicidae)
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Bacterial symbionts are common across insects, including ants (Hymenoptera: Formicidae). Reproduction-manipulating endosymbionts, such as Wolbachia, Spiroplasma, Rickettsia, and Cardinium, are closely associated with many aspects of host-insect life. In addition, phage WO plays an essential role in the phenotypic effects of Wolbachia. Although endosymbionts are possible biological control agents, there is a lack of knowledge of their rate of infection of ants in Korea. We tested a range of Korean ant species for the presence of Wolbachia, Spiroplasma, Rickettsia, Cardinium, and phage WO by extracting DNA from the ants and using specific primer sets to test the status of infections. In addition, the mitochondrial cytochrome c oxidase I (COI) gene of the host ants was amplified to confirm the molecular identification and phylogenetic relationship between the hosts. We found that infection with Wolbachia (29.6% of species) is elatively common when compared with that of other endosymbionts. Only one species was infected with Spiroplasma. Infection with Rickettsia and Cardinium was not detected in the examined ants. Most Wolbachia in ants were infected with phage WO. Although the phenotypic effects of endosymbionts in ants are still unknown, this first survey of endosymbionts in Korea is the first step toward the use of reproduction-manipulating endosymbionts.
Abstract
Bacterial symbionts are common across insects, including ants (Hymenoptera: Formicidae). Reproduction-manipulating endosymbionts, such as Wolbachia, Spiroplasma, Rickettsia, and Cardinium, are closely associated with many aspects of host-insect life. In addition, phage WO plays an essential role in the phenotypic effects of Wolbachia. Although endosymbionts are possible biological control agents, there is a lack of knowledge of their rate of infection of ants in Korea. We tested a range of Korean ant species for the presence of Wolbachia, Spiroplasma, Rickettsia, Cardinium, and phage WO by extracting DNA from the ants and using specific primer sets to test the status of infections. In addition, the mitochondrial cytochrome c oxidase I (COI) gene of the host ants was amplified to confirm the molecular identification and phylogenetic relationship between the hosts. We found that infection with Wolbachia (29.6% of species) is elatively common when compared with that of other endosymbionts. Only one species was infected with Spiroplasma. Infection with Rickettsia and Cardinium was not detected in the examined ants. Most Wolbachia in ants were infected with phage WO. Although the phenotypic effects of endosymbionts in ants are still unknown, this first survey of endosymbionts in Korea is the first step toward the use of reproduction-manipulating endosymbionts.
참고문헌 (29)
- Bordenstein, S. R., "Bacteriophage flux in endosymbionts (Wolbachia): infection frequency, lateral transfer, and recombination rates", Molecular Biology and Evolution, 21, pp.1981~1991 (2004)
- Duron, O., "The diversity of reproductive parasites among arthropods : Wolbachia do not walk alone", BMC Biology, 6, pp.27~ (2008)
- Feldhaar, H., "Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia", BMC Biology, 5, pp.48~ (2007)
- Frost, C. L., "Multiple gains and losses of Wolbachia symbionts across a tribe of fungus-growing ants", Molecular Ecology, 19, pp.4077~4085 (2010)
- Goodacre, S. L., "Wolbachia and other endosymbiont infections in spiders", Molecular Ecology, 15, pp.517~527 (2006)
- Hilgenboecker, K., "How many species are infected with Wolbachia?–A statistical analysis of current data", FEMS Microbiology Letters, 281, pp.215~220 (2008)
- Hölldobler, B., "The Ants" (1990), Belknap Press of Harvard University Press
- Jaenike, J., "Association between Wolbachia and Spiroplasma within Drosophila neotestacea: an emerging symbiotic mutualism?", Molecular Ecology, 19, pp.414~425 (2010)
- Kageyama, D., "Prevalence of a non-male-killing spiroplasma in natural populations of Drosophila hydei", Applied and Environmental Microbiology, 72, pp.6667~6673 (2006)
- Kautz, S., "Bacterial infections across the ants: frequency and prevalence of Wolbachia, Spiroplasma, and Asaia", Psyche: a Journal of Entomology, 2013, pp.936341~ (2013)
- Kumar, S., "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets", Molecular Biology and Evolution, 33, pp.1870~1874 (2016)
- LePage, D. P., "Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility", Nature, 543, pp.243~247 (2017)
- 류동표, "한국에서 Solenopsis invicta (벌목, 개미과)의 발견 보고", 한국응용곤충학회지, 56(4), pp.437~438 (2017), 한국응용곤충학회
- Martin, O. Y., "Widespread infections by the bacterial endosymbiont Cardinium in arachnids", The Journal of Arachnology, 37, pp.106~108 (2009)
- Martins, C., "Presence and distribution of the endosymbiont Wolbachia among Solenopsis spp. (Hymenoptera: Formicidae) from Brazil and its evolutionary history", Journal of Invertebrate Pathology, 109, pp.287~296 (2012)
- Masui, S., "Distribution and evolution of bacteriophage WO in Wolbachia, the endosymbiont causing sexual alterations in arthropods", Journal of Molecular Evolution, 51, pp.491~497 (2000)
- Morrison, L. W., "Potential global range expansion of the invasive fire ant, Solenopsis invicta", Biological Invasions, 6, pp.183~191 (2004)
- Nei, M., "Molecular Evolution and Phylogenetics" (2000), Oxford University Press
- Russell, J. A., "Bacterial gut symbionts are tightly linked with the evolution of herbivory in ants", Proceedings of the National Academy of Sciences of the United States of America, 106, pp.21236~21241 (2009)
- Russell, J. A., "The ants(Hymenoptera : Formicidae)are unique and enigmatic hosts of prevalent Wolbachia(Alphaproteobacteria)symbionts", Myrmecological News, 16, pp.7~23 (2012)
- Sirviö, A., "Multiple endosymbionts in populations of the ant Formica cinerea", BMC Evolutionary Biology, 10, pp.335~ (2010)
- Stouthamer, R., "Wolbachia pipientis : microbial manipulator of arthropod reproduction", Annual Review of Microbiology, 53, pp.71~102 (1999)
- 성선용, "Predicting the Potential Distribution of an Invasive Species, Solenopsis invicta Buren (Hymenoptera: Formicidae), under Climate Change using Species Distribution Models", Entomological Research, 48(6), pp.505~513 (2018), 한국곤충학회
- Stoll, S., "Bacterial microbiota associated with ants of the genus Tetraponera", Biological Journal of the Linnean Society, 90, pp.399~412 (2007)
- Treanor, D., "Limited female dispersal predicts the incidence of Wolbachia across ants (Hymenoptera: Formicidae)", Journal of Evolutionary Biology, 32, pp.1163~1170 (2019)
- Wang, N., "Multiple horizontal transfers of bacteriophage WO and host Wolbachia in fig wasps in a closed community", Frontiers in Microbiology, 7, pp.136~ (2016)
- Werren, J. H., "Wolbachia infection frequencies in insects: evidence of a global equilibrium?", Proceedings Biological Sciences, 267, pp.1277~1285 (2000)
- Werren, J. H., "Wolbachia : master manipulators of invertebrate biology", Nature Reviews Microbiology, 6, pp.741~751 (2008)
- Wojcik, D. P., "Red imported fire ants : impact on biodiversity", American Entomologist, 47, pp.16~23 (2001)