A new distribution record of Chrysosplenium grayanum Maxim. (Saxifragaceae) in Korea: Evidence from morphological and molecular data
5
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Chrysosplenium grayanum Maxim. (Series Nepalensia), which had been known to be restricted to Japan, was newly discovered from Mt. Cheongtae in Yeonggwang-gun, Jeollanam-do, located in the southern part of the Korean Peninsula. Species identification was confirmed using morphological characteristics and DNA sequence data, while comparing with materials obtained from Japan and herbarium specimens. Chrysosplenium grayanum is clearly distinguished from the remaining taxa of the genus Chrysosplenium by having glabrous plant body, opposite leaves, cylindrical papillae with roundish head at the tip on the smooth seed surface, and four stamens. Molecular sequence data of the nuclear ribosomal ITS regions, chloroplast rbcL and matK genes strongly supported that this previously unknown Chrysosplenium species from Korea is C. grayanum. Taking the molecular and the morphological evidence into consideration, it is clear that newly discovered Chrysosplenium population in Korea is conspecific with the widely distributed C. grayanum in Japan. In this paper, we provide a description, illustration, and photo images of Chrysosplenium grayanum from Korea and also a key to the Chrysosplenium species in Korea.
Abstract
Chrysosplenium grayanum Maxim. (Series Nepalensia), which had been known to be restricted to Japan, was newly discovered from Mt. Cheongtae in Yeonggwang-gun, Jeollanam-do, located in the southern part of the Korean Peninsula. Species identification was confirmed using morphological characteristics and DNA sequence data, while comparing with materials obtained from Japan and herbarium specimens. Chrysosplenium grayanum is clearly distinguished from the remaining taxa of the genus Chrysosplenium by having glabrous plant body, opposite leaves, cylindrical papillae with roundish head at the tip on the smooth seed surface, and four stamens. Molecular sequence data of the nuclear ribosomal ITS regions, chloroplast rbcL and matK genes strongly supported that this previously unknown Chrysosplenium species from Korea is C. grayanum. Taking the molecular and the morphological evidence into consideration, it is clear that newly discovered Chrysosplenium population in Korea is conspecific with the widely distributed C. grayanum in Japan. In this paper, we provide a description, illustration, and photo images of Chrysosplenium grayanum from Korea and also a key to the Chrysosplenium species in Korea.
참고문헌 (30)
- Bensel, C. R., "Floral anatomy in the Saxifragaceae sensu lato. Ⅳ. Baueroideae and conclusions", Am. J. Bot, 62(7), pp.688~694 (1975)
- Chung, T.H., "Illustrated encyclopedia of fauna & flora of Korea. Vol. 5. Tracheophyta", pp.232~ (1965), Ministry of Education
- Chung, Y. H., "Monographic study of the endemic plants in Korea 10. Taxonomy and interspecific relationships of the genus Chrysosplenium", Korean J. Environ. Biol, 6(2), pp.33~63 (1988)
- Fay, M. F., "Familial relationships of Rhabdodendron(Rhadbdodendraceae) : plastid rbcL sequences indicate a caryophyllid placement", Kew Bull, 52(4), pp.923~932 (1997)
- Felsenstein, J., "Confidence limits on phylogenies : an approach using the bootstrap", Evolution, 39(4), pp.783~791 (1985)
- 한종원, "ITS 염기서열에 의한 한국산 괭이눈속(Chrysosplenium)의 계통학적 연구", 한국자원식물학회지, 24(4), pp.358~369 (2011), 한국자원식물학회
- Hara, H., "Synopsis of the genus Chrysosplenium L., (Saxifragaceae)", J. Fac. Sci. Univ. Tokyo, Bot, 7, pp.1~90 (1957)
- "The IUCN red list of threatened species, version 2019-2" (2019)[URL]
- Kim, H.S., "Flora Coreana 3", pp.149~174 (1997), The Science and Technology Publishing House
- Kim, Y.-D., "The genera of vascular plants of Korea", pp.521~530 (2007), Academy Publishing Co
- Yong-In Kim, "Molecular Systematic Study of Chrysosplenium Series Pilosa (Saxifragaceae) in Korea", Journal of Plant Biology(한국식물학회지), 54(6), pp.396~401 (2011), 한국식물학회
- Kim, Y. -I., "Chrysosplenium aureobracteatum(Saxifragaceae), a new species from South Korea", Novon, 23, pp.432~436 (2015)
- Kim, Y. -I., "Chrysosplenium ramosissimum Y.I. Kim & Y.D. Kim (Saxifragaceae), a new species from Korea", PhytoKeys, 111, pp.1~10 (2018)
- Kimura, M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol, 16(2), pp.111~120 (1980)
- Lee, T.B., "Illustrated flora of Korea", pp.412~414 (1980), Hyangmunsa
- Lee, W.T., "Lineamenta florae Koreae", pp.1688~ (1996), Academy Publishing Co
- Lee, Y.N., "New flora of Korea, Vol. I", pp.974~ (2006), Kyohak Publishing Co
- Lee, Y. N., "An investigation on flora of Chuwangsan National Park", The Report of the KACN, 23, pp.37~61 (1985)
- Maximowicz, C.J., "Diagnosis plantarum novarum asiaticarum scripsit", Bull. Acad. Imp. Sci. Saint-Pétersbourg, 23, pp.340~350 (1877)
- Report of plant research in Jejudo and Wando, "Nakai, T.", Japanese Government-General of Korea, 156~ (1914)
- Nakai, T., "A synoptical sketch of Korean flora", Bull. Natl. Sci. Mus. Tokyo, 31, pp.1~152 (1952)
- Nakazawa, M., "Molecular phylogenetic analysis of Chrysosplenium (Saxifragaceae) in Japan", J. Plant Res, 110, pp.265~274 (1997)
- Park, M.-K., "Keys to the herbaceous plants in Korea (Dicotyledoneae)", pp.593~ (1974), Chungeumsa
- Sponberg, G.L., "The genera of Saxifragaceae in the southeastern United States", J. Arnold Arb, 53(4), pp.409~494 (1972)
- Swofford, D.L., "PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0.b10", Sinauer Associates Inc, ~ (2002)
- Thompson, J. D., "CLUSTAL W : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res, 22(22), pp.4673~4680 (1994)
- Wakabayashi, M., "Flora of Japan. Vol. IIb", pp.58~70 (2001), Kodansha
- Wakabayashi, M., "A new species of Chrysosplenium (Saxifragaceae) from central Honshu, Japan", Acta Phytotax. Geobot, 50, pp.1~12 (1999)
- White, T. J., "PCR protocols: A guide to methods and applications", pp.315~322 (1990), Academic Press, Inc
- Ye, H., "A new species of Chrysosplenium from Guangxi", Acta Bot. Austro Sin, 9, pp.57~59 (1994)