A report on 14 unrecorded bacterial species isolated from the Nakdong River, South Korea
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As a part of the research project “Survey of freshwater organisms and specimen collection,” freshwater samples were collected from the Nakdong River. Among the bacterial isolates, we selected strains that showed higher than 98.7% 16S rRNA gene sequence similarity with confirmed bacterial species previously unreported in South Korea. The 14 new records to South Korea were phylogenetically diverse and belonged to four phyla, six classes, 11 orders, and 14 genera. At the genus level, these species were found to be affiliated with Reyranella, Ferrovibrio, Brevundimonas, and Aquidulcibacter of the class Alphaproteobacteria; Pseudomonas, Cellvibrio, and Photobacterium of the class Gammaproteobacteria; Paenibacillus and Bacillus of the phylum Firmicutes; Chryseobacterium, Flavobacterium, Pedobacter of the phylum Bacteroidetes; and Actinomadura and Leifsonia of the phylum Actinobacteria. These species were further characterized by examining their Gram reaction, colony and cell morphologies, biochemical properties, and phylogenetic positions. The detailed descriptions of these 14 previously unreported species are provided.
Abstract
As a part of the research project “Survey of freshwater organisms and specimen collection,” freshwater samples were collected from the Nakdong River. Among the bacterial isolates, we selected strains that showed higher than 98.7% 16S rRNA gene sequence similarity with confirmed bacterial species previously unreported in South Korea. The 14 new records to South Korea were phylogenetically diverse and belonged to four phyla, six classes, 11 orders, and 14 genera. At the genus level, these species were found to be affiliated with Reyranella, Ferrovibrio, Brevundimonas, and Aquidulcibacter of the class Alphaproteobacteria; Pseudomonas, Cellvibrio, and Photobacterium of the class Gammaproteobacteria; Paenibacillus and Bacillus of the phylum Firmicutes; Chryseobacterium, Flavobacterium, Pedobacter of the phylum Bacteroidetes; and Actinomadura and Leifsonia of the phylum Actinobacteria. These species were further characterized by examining their Gram reaction, colony and cell morphologies, biochemical properties, and phylogenetic positions. The detailed descriptions of these 14 previously unreported species are provided.
참고문헌 (16)
- Chun, S. J., "Characterization of Distinct CyanoHABs-Related Modules in Microbial Recurrent Association Network", Frontiers in Microbiology, 10, pp.1637~ (2019)
- Edwards, R. A., "Using pyrosequencing to shed light on deep mine microbial ecology", BMC Genomics, 7, pp.57~ (2006)
- Falkowski, P. G., "The microbial engines that drive earth’s biogeochemical cycles", Science, 320, pp.1034~1039 (2008)
- Felsenstein, J., "Confidence limits on phylogenies : an approach using the bootstrap", Evolution, 39, pp.783~791 (1985)
- Huse, S. M., "Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencing", PLoS Genetics, 4, pp.e1000255~ (2008)
- Jeon, Y. S., "EzEditor : a versatile sequence alignment editor for both rRNA-and protein-coding genes", International Journal of Systematic and Evolutionary Microbiology, 64, pp.689~691 (2014)
- Kimura, M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", Journal of Molecular Evolution, 16, pp.111~120 (1980)
- Kumar, S., "MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets", Molecular Biology and Evolution, 33, pp.1870~1874 (2016)
- Miettinen, I. T., "Phosphorus and bacterial growth in drinking water", Applied and Environmental Microbiology, 63, pp.324~3245 (1997)
- Newton, R. J., "A guide to the natural history of freshwater lake bacteria", Microbiology and Molecular Biology Reviews, 75, pp.14~49 (2011)
- Saitou, N., "The neighbor-joining method : a new method for reconstructing phylogenetic trees", Molecular Biology and Evolution, 4, pp.406~425 (1987)
- 신용식, "국내 연안 하구역의 식물플랑크톤 생체량(chlorophyll a)및 수질 동향", 생태와 환경, 38(2), pp.160~180 (2005), 한국수생태학회
- Weisburg, W. G., "16S ribosomal DNA amplification for phylogenetic study", J Bacteriol, 173, pp.697~703 (1991)
- Williamson, N., "Survival of cyanobacteria in rivers following their release in water from large headwater reservoirs", Harmful Algae, 75, pp.1~15 (2018)
- Yoon, S. H., "Introducing EzBioCloud : a taxonomically united database of 16S rRNA gene sequences and wholegenome assemblies", International Journal of Systematic and Evolutionary Microbiology, 67, pp.1613~1617 (2017)
- Zhou, S., "Nitrogen removal characteristics of enhanced in situ indigenous aerobic denitrification bacteria for micro-polluted reservoir source water", Bioresource Technology, 201, pp.195~207 (2016)