Tunicamycin 이 돼지정자의 생존성, 미토콘드리아 활성, 정자의 운동성에 미치는 영향
Effect of Tunicamycin on Sperm Viability, Mitochondrial Activity and Motility in Boar Semen
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본 연구는 돼지 정액에 있어 tunicamycin(TM)이 정자의 생존성, 미토콘드리아 활성 및 운동성에 미치는 영향을 검증하였다. 채취된 돼지 정액은 0, 1, 2, 및 5μM의 TM이 함유된 정액 희석액 내에서 3, 6 및 9시간동안 배양되었다. 정자의 생존성은 SYBR14/PI 이중 염색을 이용하여 분석되었으며, 미토콘드리아 활성은 Rhodamine123/PI 염색을 이용하여 확인되었다. 정자 생존성, 미토콘드리아 활성 및 운동성은 배양시간동안 3시간마다 측정되었다. 그 결과 돼지 정자 생존성, 미토콘드리아 활성 및 운동성은 모든 배양시간동안 대조구에 비해 2 및 5μM TM 처리구들에서 유의적으로 감소하였다(p<0.05). 또한 미토콘드리아 활성 및 운동성은 배양 후 9시간 때 대조구에 비해 1, 2, 및 5μM TM 처리구에서 유의적으로 감소하였다(p<0.05). 이러한 결과는 돼지 정액에서 TM이 정자 생존성, 미토콘드리아 활성 및 운동성을 억제할 수 있으며, 정자의 수정능력에 영향을 미칠 것으로 판단된다.
Abstract
This study was to investigate effect of tunicamycin (TM) on sperm viability, mitochondrial activity and motility in boar semen. Collected sperm were incubated with semen extender containing 0, 1, 2, and 5 μM TM for 3, 6 and 9 h. Sperm viability was analyzed using SYBR14/PI doubling staining, and mitochondrial activity was detected using Rhodamine123/PI staining methods. Sperm viability, mitochondrial activity and motility were measured every 3 h during incubation. In results, boar sperm viability, mitochondrial activity and motility were significantly decreased in 2 and 5 μM TM groups compare to control group at all incubation time (p<0.05). In addition, mitochondrial activity and motility were significantly decreased in 1, 2, and 5 μM TM groups compare to control group at 9 h after incubation (p<0.05). These results suggest that TM can inhibit sperm viability, mitochondrial activity and motility in boar semen, and it may influence on the fertility of sperm.
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