산란계 사료 내 Beta-mannanase의 수준별 첨가가 산란성적, 영양소 소화율, 혈액성상, 분내 휘발성 지방산 및 암모니아태 질소 농도에 미치는 영향
Effects of Supplementation of β-Mannanase on Egg Production Performance, Nutrient Digestibility, Blood Metabolites, Excreta Volatile Fatty Acids and Ammonia Nitrogen in Laying Hens
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초록
본 연구는 산란계 사료에 β-mannanase의 적정 첨가수준을 알아보기 위하여 실시하였다. 80주령 산란계 320수(4처리, 8반복, 반복당 10수)를 공시하여 β-mannanase 4수준(0, 0.04, 0.08, 0.16%)으로 9주간 사양시험을 실시하였다. 전체 기간에 있어서 β-mannanase의 첨가는 그 수준이 증가함에 따라 산란율과 산란량이 유의적으로 개선되는 것으로 나타났다. 또한 β-mannanase 첨가 수준이 증가함에 따라 건물, 조단백질 및 칼슘 소화율이 유의적으로 개선되었으며, 특히 사료 내 mannan 소화율이 유의적으로 개선되는 것으로 나타났다. 산란계의 혈액성상, 분내 휘발성 지방산 및 NH4-N 함량은 β-mannanase 첨가에 따른 유의적인 차이는 나타나지 않았다. 결론적으로, 이 실험의 결과로 보아 산란계 사료에 대한 β-mannanase의 적정 첨가수준은 0.08%로 판단된다.
Abstract
The present study was conducted to determine the optimal supplementation level of beta-mannanase in the diet of laying hens. A total of 320 Hy-Line Brown layers (80 weeks of age) were assigned randomly into four groups on the basis of laying performance. Each treatment had eight replicates with 10 birds each (80 birds per treatment). Two hens were caged individually. Treatments were basal diet supplemented with 0 (control), 0.04, 0.08, and 0.16% beta-mannanase during the nine-week feeding period. Laying hens fed diets supplemented with increasing levels of beta-mannanase had increased (linear, p<0.05) overall egg production and egg mass. In addition, these hens had greater retention of dry matter, crude protein, gross energy, calcium, and mannan (linear, p<0.05). Dietary beta-mannanase treatments had no effect on blood metabolites such as total carbohydrate, triglycerides, glucose, total protein, and blood urea nitrogen, or excreted ammonia nitrogen and volatile fatty acids. The results obtained in present study indicate that dietary supplementation of beta-mannanase has the potential for improving the performance of laying hens. The optimal supplementation level is 0.04% beta-mannanase in the diet.
참고문헌 (23)
- AOAC., "Official Methods of Analysis" (2007), Association of Official Analytical Chemists International
- Centeno, M. S., "Galactanases and mannanases improve the nutritive value of maize and soybean meal based diets for broiler chicks", J. Poult. Sci, 43, pp.344~350 (2006)
- Chaney, A. L., "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~132 (1962)
- Cheraghi, A., "Effects of dietary levels of yeast extracted beta-glucans and alpha-mannans Alphamune (TM) on performance of broiler chicken raised in normal and thermal-stressed conditions", Eur. Poult. Sci, 78, pp.78~ (2014)
- "Soybean meal: An exceptional protein source" (2012)[URL]
- Dale, L., "Hemicell, Poultry and swine feed enzyme" (1997), ChemGen Crop
- Daskiran, M., "An evaluation of endo-β-D-mannanase (hemicell) effects on broiler performance and energy use in diets varying in β-mannan content", Poult. Sci, 4, pp.662~668 (2004)
- Dierick, N. A., "Biotechnology aids to improve feed and feed digestion: Enzyme and fermentation", Arch. Anim. Nutr. Berlin, 3, pp.241~261 (1989)
- Garry, B. P., "The effect of cereal type and enzyme addition on pig performance, intestinal microflora, and ammonia and odour emission", Animal, 1, pp.751~757 (2007)
- Hsiao, H. Y., "Levels of β-mannan in soybean meal", Poult. Sci, 85, pp.1430~1432 (2006)
- Jo, J. K., "Effects of exogenous enzyme supplementation to corn-soybean based or complex diets on growth performance, nutrient digestibility and blood metabolites in growing pigs", J. Anim. Sci, 89, pp.1795~1804 (2012)
- Kim, J. S., "Effects of energy levels of diet and β-mannanase supplementation on growth performance, apparent total tract digestibility and blood metabolites in growing pigs", Anim. Feed. Sci. Tech, 186, pp.64~70 (2013)
- 이준엽, "Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens", 한국축산학회지, 55(2), pp.115~122 (2013), 한국축산학회
- McCleary, B. V., "β-D-mannanase", Meth. Enzymol, 160, pp.596~610 (1988)
- McNaughton, J. L, "Corn", soy, Poultr. Sci, pp.1998, 77[DOI]
- NRC., "Nutrient Requirement of Poultry" (1994), National Academy Press
- Nunes, C. S., "Effects of guar gum and cellulose on glucose absorption, hormonal release and hepatic metabolism in pig", Br. J. Nutr, 68, pp.693~700 (1992)
- O’Connell, J. M., "The effect of cereal type and exogenous enzyme supplementation in pig diets on nutrient digestibility, intestinal microflora, volatile fatty acid concentration, and manure ammonia emissions from finisher pigs", Anim. Sci, 81, pp.357~364 (2005)
- SAS, "Statistical Analysis System. Users Guide:Statistics, Version 8.2", SAS Institute, ~ (2012)
- Wu, G., "Effects of beta-mannanase in corn-soy diets on commercial leghorns in second-cycle hens", Poult. Sci, 84, pp.894~897 (2005)
- Yoon, S. Y., "Effects of mannanase and distillers dried grain with solubles on growth performance, nutrient digestibility, and carcass characteristics of grower-finisher pigs", J. Anim. Sci, 88, pp.181~191 (2010)
- Zangiabadi, H. R., "The effect of a β-mannanase-based enzyme on growth performance and humoral immune response of broiler chickens fed diets containing graded levels of whole dates", Trop. Anim. Health. Pro, 42, pp.1209~1217 (2010)
- Zou, X. T., "Effect of β-mannanase (hemicell) on growth performance and immunity of broilers", Poult. Sci, 85, pp.2176~2179 (2006)