Screening of Medicinal Plants Inhibitor on Osteoclast Activity
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초록
The bone is supported by osteoblast and osteoclast interaction which are able to bone formation and bone resorption. Osteoporosis is defined by a low bone mass and caused by a relative increase of ostoclastic bone resorption over ostoblastic bone formation. Bone mineral density (BMD) are affected by mechanical and nutritional factor, leading to an risk of fractures. Activity of osteoclast is stimulated by receptor activator of nuclear factor NF-κB ligand (RANKL). Thus, the compounds inhibiting osteoclasts activity can improve bone diseases such as osteoporosis. In this study, the methanol extracts of 34 medicinal plant were screened for the inhibitory activity against RANKL-induced osteoclast differentiation and proliferation in mouse bone marrow macrophages and against the formation of tartrate-resistant acid phosphatase (TRAP). Among the methanol extracts of 34 medicinal plant tested, Angelica gigas, Melia azedarach, Saururus chinensis had considerably inhibitory effects on RANKL-induced osteoclast differentiation, and on proliferation of osteoclasts. This finding suggests that methanol extracts from Angelica gigas, Melia azedarach, Saururus chinensis may have therapeutic potential for use in the treatment of bone-related disease like osteoporosis and rheumatoid arthritis.
Abstract
The bone is supported by osteoblast and osteoclast interaction which are able to bone formation and bone resorption. Osteoporosis is defined by a low bone mass and caused by a relative increase of ostoclastic bone resorption over ostoblastic bone formation. Bone mineral density (BMD) are affected by mechanical and nutritional factor, leading to an risk of fractures. Activity of osteoclast is stimulated by receptor activator of nuclear factor NF-κB ligand (RANKL). Thus, the compounds inhibiting osteoclasts activity can improve bone diseases such as osteoporosis. In this study, the methanol extracts of 34 medicinal plant were screened for the inhibitory activity against RANKL-induced osteoclast differentiation and proliferation in mouse bone marrow macrophages and against the formation of tartrate-resistant acid phosphatase (TRAP). Among the methanol extracts of 34 medicinal plant tested, Angelica gigas, Melia azedarach, Saururus chinensis had considerably inhibitory effects on RANKL-induced osteoclast differentiation, and on proliferation of osteoclasts. This finding suggests that methanol extracts from Angelica gigas, Melia azedarach, Saururus chinensis may have therapeutic potential for use in the treatment of bone-related disease like osteoporosis and rheumatoid arthritis.
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