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정신분열병 환자의 Apolipoprotein E 유전형과 임상변인

Apolipoprotein E Genotype and Clinical Variables in Korean Schizophrenic Patients

이상수|최진영|남범우|문석우|서정석
대한생물치료정신의학회 (Korean Society Of Biological Therapies In Psychiatry)|2008.06|생물치료정신의학 (Journal of the Korean Society of Biological Therapies in Psychiatry)|제14권 제1호|pp. 65-76

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참고문헌

키워드

CognitionSchizophrenia정신분열병Apo EPolymorphism
주제분류정신과학

초록

Objectives：In the central nervous system, apolipoprotein E(Apo E) is thought to play a role in neuronal growth and repair. Numerous studies have provided evidence for a genetic association of the Apo E ε4 allele and late on¬set familial and sporadic Alzheimer’s disease. However, previous studies on Apo E genotype distribution in schizo¬phrenia, which would suffer from severe cognitive impairment, have reported conflicting findings. So we have exam¬ined the genetic association between schizophrenia and Apo E genotype polymorphism to investigate the possible contribution to schizophrenia. Methods：Genomic DNAs from 100 schizophrenic patients and 60 healthy controls were isolated. The Apo E gen ¬otype was determined by the polymerase chain reaction(PCR) with sequence-specific oligonucleotide primers (SSOP) and reverse hybridization using the INNO LiPA(Line Probe Assay) Apo E typing test kit. The frequencies of allele and genotype were compared between patients and controls. And the allele frequencies were compared in terms of positive and negative subtypes according to the PANSS, family history and age of onset. Results：The frequencies of Apo E allele and genotype were not significantly different between the patients and controls. But in patients group, the frequencies of allele and genotype between subtypes by PANSS were significantly different. Some other clinical variables such as sex, age of onset and family history were not associated with Apo E genotype. Conclusion：Apo E gene has no causal relation to the development of schizophrenia in Korean population, but it may affect the phenotype of schizophrenia.

Abstract

Objectives：In the central nervous system, apolipoprotein E(Apo E) is thought to play a role in neuronal growth and repair. Numerous studies have provided evidence for a genetic association of the Apo E ε4 allele and late on¬set familial and sporadic Alzheimer’s disease. However, previous studies on Apo E genotype distribution in schizo¬phrenia, which would suffer from severe cognitive impairment, have reported conflicting findings. So we have exam¬ined the genetic association between schizophrenia and Apo E genotype polymorphism to investigate the possible contribution to schizophrenia. Methods：Genomic DNAs from 100 schizophrenic patients and 60 healthy controls were isolated. The Apo E gen ¬otype was determined by the polymerase chain reaction(PCR) with sequence-specific oligonucleotide primers (SSOP) and reverse hybridization using the INNO LiPA(Line Probe Assay) Apo E typing test kit. The frequencies of allele and genotype were compared between patients and controls. And the allele frequencies were compared in terms of positive and negative subtypes according to the PANSS, family history and age of onset. Results：The frequencies of Apo E allele and genotype were not significantly different between the patients and controls. But in patients group, the frequencies of allele and genotype between subtypes by PANSS were significantly different. Some other clinical variables such as sex, age of onset and family history were not associated with Apo E genotype. Conclusion：Apo E gene has no causal relation to the development of schizophrenia in Korean population, but it may affect the phenotype of schizophrenia.

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