한 남자에서 수면 박탈 시 cDNA Microarray 분석에 의한 유전자 발현
Effects of Sleep Deprivation on Gene Expression by cDNA Microarray Analysis in One Male Subject
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목 적： 본 연구에서는 약 80개 이상의 사람의 주요 유전자를 검색할 수 있는 TwinChip Human 8K cDNA miceoaray를 이용하여, 수면 박탈이 이러한 주요 유전자의 발현 양상에 어떠한 영향을 미치는 지를 대량으로 확인하고 분석하고자 하였다. 방 법： Mendelson이 시행한 수면 박탈 방법을 변형하여 4일간의 일정으로 수면 박탈을 시행하였으며 첫째날(ba -seline) 수면 박탈 시행전과 4일째 수면 박탈 실험 완료후에 각각 혈액채취를 하였다. 수면 박탈전과 수면 박탈후에 채취한 혈액을 대상으로 단핵세포를 분리하여 RNA를 추출후 TwinChip Human 8K cDNA micro-aray 반응을 시킨 후 결과를 분석하였다.
Abstract
bjective：The purpose of this study was to evaluate the effect of sleep deprivation on gene expression by cDNA microarray analysis. Methods：Subject of this study is one 27 years old male, physically and psychologically healthy without sleep/wake complaints. Baseline blood sample was done at 09：00 of the day 1. Subject slept 4 hours on the day 1, 2 hours on the day 2, and total sleep deprivation on the day 3. Blood samples was done in the morning of the day 4. Using cDNA microarray technique, gene expression profile was assayed. Results：There were 8 genes up-regulated and 9 genes down-regulated after sleep deprivation. Up-regulated genes were NTS(neurotensin), RHAG(Rhesus blood group-associated glycoprotein), CLIPR-59(CLIP-170related protein), FLJ0849(hypothetical protein FLJ10849), Homo sapiens TTF-I interacting peptide 20mRNA, GRM3 (glutamate receptor, metabotropic 3), ELK3, ETS-domain protein(SRF accessory protein 2), RBMS1(RNA binding motif, single stranded interacting protein 1), and down-regulated genes were ESTs, IL-2(interleukin-2), ATP6V0A1 (ATPase, H+transporting, lysosomal V0 subunit a isoform), Homo sapiens cDNS FLJ31377 fis, clone NESOP100008, CYP11A(cytochrome P450, subfamily XIA cholesterol side chain)), RECQL4(RecQ protein-like 4), IGSF3(immunoglobulin superfamily, member 3), CGI-94(CGI-94 protein), CALR(calreticulin). Conclusion：The results of the present study cannot fully explain the effects of sleep deprivation with respect to the expression of certain genes, but they provide fundamental data for the involvement of sleep deprivation on gene expression profiles. Further in-depth studies are required to confirm the differential expression of these genes by RT-PCR or Western blot.