영상 자료 획득시의 오류가 전자토모그래피 결과에 미치는 영향 고찰-모델 시뮬레이션을 중심으로
Model Simulation for Assessment of Image Acquisition Errors Affecting Electron Tomography
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참고문헌
키워드
초록
This simulation study examined the effect of data acquisition error including the data type of TEM image, and incident beam intensity of the tilt series on 3D tomograms. Simulation was performed with the 3D head phantom model of Kak and Slaney, and the slightly modified 3D head phantom model with enhanced difference in absorption coefficients. Reconstructed tomogram for the original head phantom model using 8-bit gray-scale image was distorted with extremely high level of noise, while an acceptable result was obtained for the modified model. The results for the original model using wrong formulation for the transmitted beam intensity was proved to be incorrect. The high level of noise along the z direction was found in case of the modified model. On the other hand, the wrong value of incident beam intensity in both models gave distorted results. In order to reconstruct an artifacts-free 3D structure from the projections with invisible features in electron tomography, the 16-bit projection images should be used with the correct incident beam intensity which is applied to Beer’s law.
Abstract
This simulation study examined the effect of data acquisition error including the data type of TEM image, and incident beam intensity of the tilt series on 3D tomograms. Simulation was performed with the 3D head phantom model of Kak and Slaney, and the slightly modified 3D head phantom model with enhanced difference in absorption coefficients. Reconstructed tomogram for the original head phantom model using 8-bit gray-scale image was distorted with extremely high level of noise, while an acceptable result was obtained for the modified model. The results for the original model using wrong formulation for the transmitted beam intensity was proved to be incorrect. The high level of noise along the z direction was found in case of the modified model. On the other hand, the wrong value of incident beam intensity in both models gave distorted results. In order to reconstruct an artifacts-free 3D structure from the projections with invisible features in electron tomography, the 16-bit projection images should be used with the correct incident beam intensity which is applied to Beer’s law.
참고문헌 (11)
- De Rosier DJ, "Reconstruction of three dimensional structures from electron micrographs", Nature, 217, pp.130~134 (1968)
- Kak AC, "Image reconstruction from projections. In handbook of pattern recognition and image processing" (1986), Academic Press
- Kak AC, "Principles of computerized tomographic imaging" (1988), IEEE Press
- Koay CG, "Three-dimensional analytical magnetic resonance imaging phantom in the fourier domai", Magn Reson Med, 58, pp.430~436 (2007)
- Menke W, "Geophysical data analysis: discrete inverse theory", pp.289~ (1989), Academic Press
- Nolet G, "Seismic tomography, with applications in global seismology and exploration geophysics", pp.386~ (1987), D. Reidel Publishing Company
- Pozsgai I, "Thickness determination by measuring in the TEM at 200 kV", Ultramicroscopy, 68, pp.69~75 (1997)
- Reynolds JM, "An introduction to applied and environmental geophysics", pp.796~ (1997), John Wiley & Sons
- Shepp LA, "Computerized tomography and nuclear magnetic resonance", J Comput Assist Tomogr, 4, pp.94~107 (1980)
- Shepp LA, "The Fourier reconstruction of a head section", IEEE Trans Nucl Sci, NS-21, pp.21~43 (1974)
- Steppe K, "Use of X-ray computed microtomography for non-invasive determination of wood anatomical characteristics", J Struct Biol, 148, pp.11~21 (2004)