Some living eukaryotes during and after scanning electron microscopy
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Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.
Abstract
Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.
참고문헌 (17)
- T. C. Boothby, "Tardigrades use intrinsically disordered proteins to survive desiccation", Mol. Cell, 65, pp.975~984 (2017)
- N. de Jonge, "Live cell electron microscopy is probably impossible", ACS Nano, 10, pp.9061~9063 (2016)
- K. He, "Imaging of soft materials using in situ liquid-cell transmission electron microscopy", J. Phys. : Condens. Matter, 31, pp.103001~ (2019)
- J. D. Hibshman, "Mechanisms of desiccation tolerance: themes and variations in brine shrimp, roundworms, and tardigrades", Front. Physiol, 11, pp.592016~ (2020)
- Y. Ishigaki, "Observation of live ticks (Haemaphysalis flava) by scanning electron microscopy under high vacuum pressure", PLoS One, 7, pp.e32676~ (2012)
- S. Kaminskyj, "Could be fungi be living on Mars? J", Astrobiol, 7, pp.6~10 (2021)
- Y. Kaneko, "A study of living plant specimens by low-temperature scanning electron microscopy", J. Elec. Microsc. Tech, 2, pp.1~6 (1985)
- E. Kennedy, "Gene expression in electronbeam- irradiated bacteria in reply to “Live cell electron microscopy is probably impossible”", ACS Nano, 11, pp.3~7 (2017)
- K. Koo, "Live cell electron microscopy using graphene veils", Nano Lett, 20, pp.4708~4713 (2020)
- H. Nishiyama, "Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film", J. Struct. Biol, 169, pp.438~449 (2010)
- R. F. Pease, "Electron microscopy of living insects", Science, 154, pp.1185~1186 (1966)
- D. Persson, "Extreme stress tolerance in tardigrades : surviving space conditions in low earth orbit", J. Zool. Syst. Evol. Res, 49(Suppl. 1), pp.90~97 (2011)
- W. P. Putnam, "Noninvasive electron microscopy with interction-free quantum measurements", Phys. Rev. A, 80, pp.040902~ (2009)
- N. D. Read, "Examination of living fungal spores by scanning electron microscopy", Expt. Mycol, 15, pp.132~139 (1991)
- D. Scharf, "Viewing and recording live hydrated specimens with a high-vacuum SEM", Micros. Today, 25, pp.12~17 (2017)
- Y. Takaku, "A thin polymer membrane, nano-suit, enhancing survival across the continuum between air and high vacuum", Proc. Natl. Acad. Sci. USA, 110, pp.7631~7635 (2013)
- H. Wu, "Liquidphase electron microscopy for soft matter science and biology", Adv. Mater., 32, pp.e2001582~ (2020)