알코올 전처치한 흰쥐에 Cyclohexane 투여로 인한 간 세포 내 소포체 미세구조의 변화
Ultrastructural changes of endoplasmic reticulum on hepatocytes by cyclohexane injection in alcohol-pretreated rats
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초록
주류의 섭취가 산업화학물질의 생체 내 독성유발에 어떠한 영향을 미치는지를 검토하기 위해 흰쥐를 이용하여 15% 에탄올을 6주간 섭취시킨 후, cyclohexane(CH)을 2일 간격으로 4회 복강 투여하고 24시간 후 다음과 같은 결과를 얻었다.CH의 투여로 인해 정상군과 비교하여 간 무게와 혈청 내 xanthine oxidase 활성은 증가되었고, 간 내 glucose-6-phosphatase 활성은 감소되었다. 그리고 CH 대사효소인 cytochrome P450 dependent aniline hydroxylase와 alcohol dehydrogenase의 활성은 CH 투여 후 유의적으로 증가되었으며, 알코올 전처치 후 CH 투여군이 가장 높은 활성을 보였다. 미세구조적으로도 알코올 섭취군과 CH 투여군 모두에서 조면소포체로부터 활면소포체로의 전환이 일어났으며, 전 실험군 중 알코올 전처치 후 CH 투여군이 가장 높은 전환율을 보였다.이러한 결과들로 볼 때, 병변을 초래하지 않는 수준의 알코올의 섭취는 CH 대사활성을 증대시키는 것으로 판단되었다.
Abstract
To evaluate the effects of ingestion of alcoholic drinks on the toxicities of industrial compounds, cyclohexane (CH) was intraperitoneally administrated to rats (1.56 g/kg body weght), which had been ingested 15% ethanol for up to to 6 weeks, 4 times by once a day and every other day. Following the last treatment of ethanol or CH, blood and liver tissues were collected during 24 hours prior to sacrifice of animals.By the injection of CH, liver weight (% of body weight) and xanthine oxidase activity in serum were increased, and glucose-6-phasphatase (G6P) activity in liver was decreased compared to them of control group. The activities of CH metabolizing enzymes, such as cytochrome P450 dependent aniline hydroxylase (CYPdAH) and alcohol dehydrogenase (ADH), were significantly increased by injection of CH, and those activities were the highest in CH-injected group after pretreated with alcohol. Ultrastructurally, both of alcohol treatment and CH injection induced transforming into the smooth-endoplasmic reticulum from rough- endoplasmic reticulum, the those rate was the highest in case of CH-injection after pretreated with alcohol.From these results, it is suggested that alcohol intake on a level without alcoholic degeneration of hepatocytes could enhance the CH metabolism of liver.
참고문헌 (25)
- "Methods of enzymatic analysis" (1974), Academic Press
- "Evaluation of the subacute nephrotoxicity of cyclohexane and other industrial solvents in the female Sprague-Dawley rat", 45, pp.271~280 (1989)
- "p-nitroanisole reversal of aceton enhanced aniline hydroxylation", pp.311~317 (1982)
- "Increased carbon tetrachloride hepatotoxicity and its metabolism after chronic ethanol consumption", pp.415~66 422 (1974)
- "Effect of chronic ethanol administration in bromobenzene liver toxicity in the rat", pp.66~77 (1983)
- "Effect of cyclohexane treatment on the liver cytochrome P-450 dependent aniline hydroxylase activity in alcohol-pretreated rats", 29, pp.23~28 (2003)
- "Ethanol metabolism, cirrhosis and alcoholism", 257(1), pp.59~84 (1997)
- "metabolic and toxic effects of ethanol", pp.573~592 (1991)
- "Effects of ethanol on the kinetics of methyl ethyl ketone in man", pp.325~330 (1990)
- "Absorption and alveolar excretion of cyclohexane in workers in a shoe factory", pp.220~223 (1981)
- "Cytochrome P450-related differences between rats and mice in the metabolism of benzene toluene and trichloroethylene in liver microsomes", pp.1079~1085 (1993), Elovaara E
- "Lung injury after hepatoenteric ischemia-reperfusion role of xanthine oxidase", 154, pp.1364~1369 (1996)
- "Implication of free radical mechanisms in ethanol-induced cellular injury", 12, pp.219~240 (1992)
- "Lung uptake and metabolism of cyclohexane in shoe factory workers", 45, pp.261~269 (1980)
- "Diminished rates of glucuronidation and sulation in perfused rat liver after chronic ethanol administration", 35, pp.439~447 (1986)
- "A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase", 28, pp.58~63 (1957)
- "Influence of ethanol or albumin metabolism edited by M", pp.79~100 (1977), Plenum Press
- "Dose related effects of single dose of ethanol on the metabolism in rat liver of some aromatic and chlorinated hydrocarbons", 60, pp.8~15 (1981)
- "Injury by drugs and toxins Electron Microscopy in Human Disease edited by J", pp.89~116 (1979), McGraw-Hill
- "Exposure to organic solvents-I A survey on the use of solvents The Annuals of Occupational Hygiene 34", pp.371~378 (1990)
- "Ethanol enhancement of cocaine-induced hepatotoxicity", 30, pp.453~458 (1980)
- "The pharmacokinetics and macromolecular interactions of trichloroethylene in mice and rats", 62, pp.137~152 (1982)
- "A kinetic photometric method for serum gamma-glutamyl transpeptidase", 15, pp.124~136 (1969)
- "Exposure monitoring and health effect studies of workers occupationally exposed to cyclohexane vapor", 65, pp.343~350 (1994)
- "A modified colormetric assay for xanthine oxidase in rat liver extracts", pp.295~308 (1984)