C57BL/6 마우스에서 나노밀 가공된 초미세분말(상황버섯)의 식이 안전성 연구
The Food Safety of Superfine Powder (Phellinus linteus) Processed by Nanomill in C57BL/6 Mice
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초록
상황버섯(Phellinus linteus)은 건강 증진 및 질병의 치료를 목 적으로 널리 사용되는 약용버섯으로서 항산화효과와 항암효과를 비롯한 다양한 생물학적 활성을 갖는다. 상황버섯은 주로 추출물 이나 분말의 형태로 복용되고 있으며, 최근 분쇄기법의 발달로 초미세분말 형태의 제품이 상용화되면서 식이 안전성에 대한 연 구의 필요성이 제기되었다. 본 연구는 상황버섯 초미세분말과 일 반 미세분말을 동물에게 먹인 후 입자의 크기에 따른 식이안전 성을 평가하기 위해 실시되었다. 부피평균에 의한 입도분석 결 과, 상황버섯 초미세분말(SPL)과 미세분말(FPL) 입자의 평균지 름은 각각 11.8 μm와 216.1 μm였고, d (0.5)는 각각 5.5 μm와 147.9 μm였다. 동물의 체중, 사료 섭취량 및 각 기관의 무게를 관 찰한 결과 SPL군은 정상군(N)이나 FPL군과 비교하여 통계학적 차이를 나타내지 않았다. 혈액학 및 생화학적 검사에서는 ALT (N vs. FPL, P<0.001)와 BUN(N vs. FPL, P<0.01; N vs. SPL, P <0.01)이 정상군과 비교하여 차이를 보였으나 FPL군과 SPL군 사이의 유의성은 발견되지 않았으며, 그 밖의 다른 검사항목들은 모두 정상범위 내에 포함되었다. 또한 조직학적 검사 결과에서도 비정상적인 소견은 관찰되지 않았다. 이 연구 결과는 초미세 가 공된 상황버섯을 경구투여할 경우 혈액학, 생화학, 조직학적인 측면에서 동물에게 유해하지 않다는 것을 말해준다. 그러나 다량 의 초미세입자가 소화기와 장기간 접촉할 경우 일어날 수 있는 조직학적 유해성에 대한 심도 깊은 연구는 필요할 것으로 판단 된다.
Abstract
A officinal mushroom, Phellinus linteus (PL) has been known to exhibit potent biological activities including antioxidative and anticancer effect. PL is consumed as a type of powder or extract for the purpose of health promotion and disease treatment. Recently superfine PL products was commercialized according to the development of pulverizing technology such as nanomill, so the evaluation of food safety is suggested. This study was conducted to evaluate the food safety of superfine PL (SPL) through hematological, biochemical and histological examination in mice as compared with fine PL (FPL). In the particle size distribution in volume after nanomill processing, the mean diameter of SPL and FPL particles was 11.78 μm and 216.1 μm, and d (0.5), the particle diameter measured at 50% of distribution was 5.5 μm and 147.9 μm, respectively. As the result of body weight, food intake and the weight of organs, SPL group didn’t show any statistical difference compared with FPL group and normal group (N). Hematological and biochemical values were also involved in the normal range, although ALT (N vs. FPL, P<0.001) and BUN (N vs. FPL, P<0.01; N vs. SPL, P<0.01) showed significance compared with N group but there are no significance between FPL and SPL group. In the result of histological examination with liver, kidney, spleen, and small and large intestine, abnormal findings such as inflammatory reaction and histological changes were not observed. Our results suggest that the oral intake of SPL diet is not harmful to the animal in the hematological, biochemical and histological aspects although particle size was reduced to the level of superfine. However, further study will be necessary to confirm the histological safety in relation to the gastrointestinal contact of superfine particles in the case of large amount and long-term intake.
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