랫드 척수신경 결찰에 따른 척수신경절세포의 퇴행성변화
Degenerative Changes of the Rat Dorsal Root Ganglion (DRG) Cells Following a Tight Spinal Nerve Ligation
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본 연구는 잘 알려진 통증모델을 대상으로 척수신경절세포의 변성과정을 경시적으로 관찰하고자 하였다. 10주령된 Sprague- Dawley 계통의 랫드를 실험동물로 사용하였고, pentobarbital (50 mg/kg)로 마취상태에서 다섯째 허리신경(L5)의 앞가지를 결찰 한 후 1일, 3일, 7일 실험군으로 구분하였다. 실험 1일과 3일군에서 작은 신경절세포에서 퇴행성변화가 먼 저 관찰되었고, 중간 및 큰 신경절세포에서는 그 변화가 미약했 다. 7일 실험군에서는 작은 신경절세포의 수가 크게 감소하였던 반면, 중간 및 큰 신경절세포에서는 큰 변화가 없었다. 전자현미 경 소견으로는 작은 신경절세포의 경우 초기 1일 및 3일군에서 미토콘드리아의 능선의 변형과 부종이 관찰되었으며, 세포질은 검게 변성되었다. 반면 큰 신경절세포의 경우 모든 실험군에서 변형된 세포가 관찰되지 않았으며, 소기관들도 잘 보존되었다. 이상의 결과를 요약하면, 척수신경 결찰에 의한 통증자극으로 랫드 척수신경절 내 작은 신경절세포는 다른 신경절세포에 비해 퇴행성변화가 빠르게 나타났으며, 미토콘드리아 등 세포질소기 관의 부종을 동반한 암적화변성(dark degeneration)을 통해 사멸 되었다.
Abstract
This study aim to disclose a possible mechanism for the neuronal cell death induced by peripheral nerve injury following a spinal nerve ligation (SNL) as a neuropathic pain model. Male Sprague-Dawley rats (270~290 g) were used for this study. Pain threshold was evaluated for their response to mechanical (von Frey hairs) stimuli 1, 3, and 7 days after a tight ligation of L5 ventral ramus. In control group, the small ganglion cells were strongly stained with routine toluidine blue (TB), whereas the large ganglion cells showed a little bit weak stainity. Each large ganglion cell is surrounded by perineuronal satellite cells. In experimental groups, small ganglion cells showing apparent degenerative changes increased on 1 day, and showed a peak in degenerative cell number at 3 days group, and decreased gradually at 7 days group. We also found a small number of large-sized ganglion cells showing mild degenerative changes. However their satellite cells ware relatively intact with no typical findings throughout this experiment. Under the electron microscope, small ganglion cells showed various stage and typical features of the dark degeneration including mitochondrial swelling.
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