Evidence for the Drp1-dependent Mitochondrial Fission in the Axon of the Rat Cerebral Cortex Neurons
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Neurons utilize a large quantity of energy for their survival and function, and thereby require active mitochondrial function. Mitochondrial morphology shows dynamic changes, depending on the cellular condition, and mitochondrial dynamics are required for neuronal development and function. In this study, we found that the length of mitochondria in the distal axon is significantly shorter than that of mitochondria in dendrites or proximal axons of cerebral cortical neurons, and the reason for this difference is the local fission within the axon. We also found that suppression of Drp1, a key regulator of mitochondrial fission, resulted in significant elongation of mitochondria in axons. Collectively, these results suggest that local mitochondrial fission within the axon contributes to region-dependent mitochondrial length differences in the axons of cortical neurons.
Abstract
Neurons utilize a large quantity of energy for their survival and function, and thereby require active mitochondrial function. Mitochondrial morphology shows dynamic changes, depending on the cellular condition, and mitochondrial dynamics are required for neuronal development and function. In this study, we found that the length of mitochondria in the distal axon is significantly shorter than that of mitochondria in dendrites or proximal axons of cerebral cortical neurons, and the reason for this difference is the local fission within the axon. We also found that suppression of Drp1, a key regulator of mitochondrial fission, resulted in significant elongation of mitochondria in axons. Collectively, these results suggest that local mitochondrial fission within the axon contributes to region-dependent mitochondrial length differences in the axons of cortical neurons.
참고문헌 (19)
- Amiri M, "Mitochondrial biogenesis in the axons of vertebrate peripheral neurons", Dev Neurobiol, 68, pp.1348~1361 (2008)
- Bereiter-Hahn J, "Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria", Microsc Res Tech, 27, pp.198~219 (1994)
- Chan DC, "Mitochondrial fusion and fission in mammals", Annu Rev Cell Dev Biol, 22, pp.79~99 (2006)
- Collins TJ, "Mitochondria are morphologically and functionally heterogeneous within cells", EMBO J, 21, pp.1616~1627 (2002)
- Davies VJ, "Opa1 deficiency in a mouse model of autosomal dominant optic atrophy impairs mitochondrial morphology, optic nerve structure and visual function", Hum Mol Genet, 16, pp.1307~1318 (2007)
- Delettre C, "Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy", Nat Genet, 26, pp.207~210 (2000)
- Eun B, "Induction of neuronal apoptosis by expression of Hes6 via p53-dependent pathway", Brain Res, 1313, pp.1~8 (2010)
- Gomes LC, "During autophagy mitochondria elongate, are spared from degradation and sustain cell viability", Nat Cell Biol, 13, pp.589~598 (2011)
- Ishihara N, "Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice", Nat Cell Biol, 11, pp.958~966 (2009)
- Knott AB, "Mitochondrial fragmentation in neurodegeneration", Nat Rev Neurosci, 9, pp.505~518 (2008)
- Li Z, "The importance of dendritic mitochondria in the morphogenesis and plasticity of spines and synapses", Cell, 119, pp.873~887 (2004)
- Mattson MP, "Mitochondria in neuroplasticity and neurological disorders", Neuron, 60, pp.748~766 (2008)
- Smirnova E, "Dynaminrelated protein Drp1 is required for mitochondrial division in mammalian cells", Mol Biol Cell, 12, pp.2245~2256 (2001)
- Smirnova E, "A human dynamin-related protein controls the distribution of mitochondria", J Cell Biol, 143, pp.351~358 (1998)
- Taguchi N, "Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission", J Biol Chem, 282, pp.11521~11529 (2007)
- Tait SW, "Mitochondria and cell death: outer membrane permeabilization and beyond", Nat Rev Mol Cell Biol, 11, pp.621~632 (2010)
- Tondera D, "SLP-2 is required for stress-induced mitochondrial hyperfusion", EMBO J, 28, pp.1589~1600 (2009)
- Wakabayashi J, "The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice", J Cell Biol, 186, pp.805~816 (2009)
- Yu T, "Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology", Proc Natl Acad Sci U S A, 103, pp.2653~2658 (2006)