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자궁근종세포의 최적 초기배양 조건 확립: 정상 자궁근세포와 자궁근종세포의 스테로이드에 대한 반응

Development of a primary tissue culture method having greater reliability than isolated cell cultures: steroid responsiveness of uterine normal myometrial and myomatous (leiomyomatous) cells

조경현|최인호|이택후|현진희|이동목|송건호|이은주|전상식|프라티|김항진
한국발생생물학회 (The Korea Society Of Developmental Biology)|2007.12|발생과 생식 (DEVELOPMENT & REPRODUCTION)|제11권 제3호|pp. 205-217

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키워드

Estrogen자궁근종유전자 발현여성호르몬세포배양Cell culturemyoma (leiomyoma or fibroid)Uterine tumor
주제분류생물학

초록

본 연구는 자궁근종 성장에 관한 분자생물학적인 기전의 이해를 위해 자궁근종 및 정상 자궁근세포의 초기 배양 방법을 확립하기 위해 실시하였다. 이를 위해 최종적으로 두 가지 세포 배양 방법이 확립되었다. 가장 안정적으로 연구(특히, 여성호르몬에 대한 반응 연구)에 사용할 수 있는 가장 적합한 세포배양방법이 모색되었다. 두 가지 세포 배양 조건 중 두 번째 방법(method 2)이 안정적으로 세포의 반응을 연구하는데 더 나은 방법으로 결론 내려졌고, 여성호르몬에 대한 반응이 더 좋은 것으로 밝혀졌다. 이 방법을 통해 배양된 세포에 E2 를 처리했을 때 정상 자궁근세포에 비해 근종세포에서 PR, IGF-1 and IGF-1 receptor mRNA의 발현이 더 높은 것으로 나타났다. 더욱이 주변에 있는 세포들 보다는 조직에서 좀 더 큰 반응을 보였으며, 이는 E2 에 대한 세포의 반응에 세포외기질 (extracellular matrix)과 세포 사이의 상호작용이 필요하다는 것을 의미한다. 결론적으로 이러한 근종세포 및 조직의 초기 배양 방법은 in vitro 상에서 종양 발생에 대한 기초 연구를 위해 유용하게 사용될 수 있을 것으로 사료된다.

Abstract

Purpose: Development of an optimal primary culture condition to understand the molecular mechanism of myoma growth. Method: Myoma and myometrium were cultured by two different methods. Culture stability and E2 responsiveness in stable culture were studied. Result: The culture of digested tissue pieces (Method 2) was found to be a stable culture method for the myoma and myometrium showing a favorable response to estrogen. mRNA expression of PR, IGF-1 and IGF-1 receptor genes was enhanced by E2. The gene responses to E2 were higher in myoma compared with myometrium. Moreover, these responses were more expressive in tissues than in the surrounding cells in primary culture of normal myometrium and myoma implying a vital role of cell communication through the extracellular matrix in maintaining the estrogen-responsiveness. Conclusion: the development of an improved cell culture system for myoma provides an in vitro tool to further investigate the basis of the tumor formation.

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관련 논문AI추천

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