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Hepatic differentiation of human eyelid adipose-derived stem cells

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A variety of stem cells has been emerging as therapeutic cells that can replace organ transplantation in human liver diseases. The present study focused on whether human eyelid adipose-derived stem cells (HAD) might differentiate into functional hepatocyte-like cells in vitro. HAD were isolated from human eyelid adipose tissue. Effect of dimethyl sulfoxide (DMSO), fibroblast growth factor (FGF)-2 and FGF-4 on the hepatic differentiation of HAD have been examined in vitro. Immunocytochemical analysis and PAS staining showed that HAD cultured in both DMSO and FGF-4 exhibited the most intense staining than HAD of the other experimental groups. These HAD expressed numerous hepatocyte-related genes. Immunoblotting analyses showed that HAD cultured in the presence of DMSO and FGF-4 secreted higher amount of human albumin than HAD cultured in other conditions. Urea analysis also demonstrated that these HAD produced higher amount of urea than any other groups of HAD. In conclusion, combined treatment of DMSO and FGF-4 could effectively induce the functional differentiation of HAD into hepatocyte-like cells.

Abstract

A variety of stem cells has been emerging as therapeutic cells that can replace organ transplantation in human liver diseases. The present study focused on whether human eyelid adipose-derived stem cells (HAD) might differentiate into functional hepatocyte-like cells in vitro. HAD were isolated from human eyelid adipose tissue. Effect of dimethyl sulfoxide (DMSO), fibroblast growth factor (FGF)-2 and FGF-4 on the hepatic differentiation of HAD have been examined in vitro. Immunocytochemical analysis and PAS staining showed that HAD cultured in both DMSO and FGF-4 exhibited the most intense staining than HAD of the other experimental groups. These HAD expressed numerous hepatocyte-related genes. Immunoblotting analyses showed that HAD cultured in the presence of DMSO and FGF-4 secreted higher amount of human albumin than HAD cultured in other conditions. Urea analysis also demonstrated that these HAD produced higher amount of urea than any other groups of HAD. In conclusion, combined treatment of DMSO and FGF-4 could effectively induce the functional differentiation of HAD into hepatocyte-like cells.

참고문헌 (36)

  1. Assmus B, "Transplantation of progenitor cells and regeneration enhancement in acutemyocardial infarction", Circulation, 106, pp.3009~3017 (2002)
  2. Banas A, "Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes", Hepatology, 46, pp.219~228 (2007)
  3. Chan TS, "The biosynthesis of ascorbate protects isolated rat hepatocytes from cumene hydroperoxide-mediated oxidative stress", Free Rad Bio Med, 38, pp.867~873 (2005)
  4. Columbano, A, "Effects of cell proliferation and cell death (apoptosis and necrosis) on the early stages of rat hepatocarcinogenesis", Carcinogenesis, 17, pp.395~400 (1996)
  5. Dicker A, "Functional studies of mesenchymal stem cells derived from adult human adipose tissue", Exp Cell Res, 308, pp.283~290 (2005)
  6. Gupta S, "Hepatocytic transplantation: emerging insights into mechanisms of liver repopulation and their relevance to potential therapies", J Hepatol, 30, pp.162~170 (1999)
  7. Hamazaki T, "Hepatic maturation in differentiating embryonic stem cells in vitro", FEBS Lett, 497, pp.15~19 (2001)
  8. Im GI, "Do adipose tissue–derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells?", Osteoarthr Cartilage, 13, pp.845~853 (2005)
  9. Jones EA, "Hepatic differentiation of murine embryonic stem cells", Exp Cell Res, 272, pp.15~22 (2002)
  10. Jung J, "Initiation of mammalian liver development from endoderm by fibroblast growth factors", Science, 284, pp.1998~2003 (1999)
  11. Kamiya A, "Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer", EMBO J, 18, pp.2127~2136 (1999)
  12. Kamiya A, "Oncostatin M and hepatocyte growth factor induce hepatic maturation via distinct signaling pathways", FEBS Lett, 492, pp.490~494 (2001)
  13. Kamiya A, "Maturation of fetal hepatocytes in vitro by extracellular matrices and oncostatin M: induction of tryptophan oxygenase", Hepatology, 35, pp.1351~1359 (2002)
  14. Kaufmann PM, "Influence of pancreatic islets on growth and differentiation of hepatocytes in co-culture", Tissue Eng, 5, pp.583~596 (1999)
  15. Kinoshita T, "Cytokine regulation of liver development", Biochim Biophys Acta, 1592, pp.303~312 (2002)
  16. Malhi H, "Isolation of human progenitor liver epithelial cells with extensive replication capacity and differentiation into mature hepatocytes", J Cell Sci, 115, pp.2679~2688 (2002)
  17. Michalopoulos GK, "HGF-, EGF-, and dexamethasone-induced gene expression patterns during formation of tissue in hepatic organoid cultures", Gene Expr, 11, pp.55~75 (2003)
  18. Miro JM, "Management of end stage liver disease (ESLD): What is the current role of orthotopic liver transplantation (OLT)?", J Hepatol, 44, pp.S140~S145 (2006)
  19. Miyazaki M, "Improved conditions to induce hepatocytes from rat BM cells in culture", Biochem Biophys Res Commun, 298, pp.24~30 (2002)
  20. Newsome PN, "Human cord blood-derived cells can differentiate into hepatocytes in the mouse liver with no evidence of cellular fusion", Gastroenterology, 124, pp.1891~1900 (2003)
  21. Nussler A, "Present status and perspectives of cell-based therapies for liver diseases", J Hepatol, 45, pp.144~159 (2006)
  22. Oh SH, "Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro", Biochem Biophys Res Commun, 279, pp.500~504 (2000)
  23. Pittenger MF, "Multilineage potential of adult human mesenchymal stem cells", Science, 284, pp.143~147 (1999)
  24. Reubinoff BE, "Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro", Nat Biotechnol, 18, pp.399~404 (2000)
  25. Sakai Y, "Enhanced in vitro maturation of fetal mouse liver cells with oncostatin M, nicotinamide and dimethyl sulfoxide", Cell Transplant, 11, pp.435~441 (2002)
  26. Sanchez A, "Fibronectin regulates morphology, cell organization and gene expression of rat fetal hepatocytes in primary culture", J Hepatol, 32, pp.242~250 (2000)
  27. Schwartz RE, "Verfaillie CM (2002) Multipotent adult progenitor cells from BM differentiate into functional hepatocyte-like cells", J Clin Invest, 109, pp.1291~1302 (2002)
  28. Seo MJ, "Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo", Biochem Biophys Res Commun, 328, pp.258~264 (2005)
  29. Shi XL, "In vitro differentiation of mouse BM mononuclear cells into hepatocyte- like cells", Hepatology Res, 31, pp.223~231 (2005)
  30. Stenderup K, "Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells", Bone, 33, pp.919~926 (2003)
  31. Strem BM, "Multipotential differentiation of adipose tissue-derived stem cells", Keio J Med, 54, pp.132~141 (2005)
  32. Talens-Visconti R, "Gomez-Lechon MJ (2006) Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells", World J Gastroenterol, 12, pp.5834~5845 (2006)
  33. Tong JZ, "Long-term culture of rat liver cell spheroids in hormonally defined media", Exp Cell Res, 189, pp.87~92 (1990)
  34. Wells JM, "Early mouse endoderm is patterned by soluble factors from adjacent germ layers", Development, 127, pp.1563~1572 (2000)
  35. Wollert KC, "Intracoronary autologous bone-marrow cell transfer after myocardial infarction: the BOOST randomised controlled clinical trial", Lancet, 364, pp.141~148 (2004)
  36. Zaret KS, "Regulatory phases of early liver development: paradigms of organogenesis", Nat Rev Genet, 3, pp.499~512 (2002)

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